Expression of Aquaporin-1 in the Pleura in Rats and the Role of Aquaporin-1 in the Pleural Fluid Turnover_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

LIAO Huai ,  XIE Canmao

Department of Respiratory Disease, The First Affiliated Hospital of Sun Yat-sen University. Guangzhou, Guangdong, 510080, China;

Corresponding author：

XIE Canmao, Email: xiecanmao@163.com

Keywords：

Aquaporin-1; Pleura; Pleural effusion; Dexamethasone; Lipopolysaccharide; Erythromycin; Hypertonic saline

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Objective To investigate the expression of aquaporin-1( AQP1 ) in visceral and parietal pleura in SD rats and to examine the effect of AQP1 on pleural fluid turnover. Methods Five groups( n = 24 ) of SD rats were randomly assigned to received intrapleural injection of dexamethasone,lipopolysaccharide, erythromycin, hypertonic saline and normal saline, respectively. The AQP1 protein in pleural was detected with immunohistochemistry. The mRNA expression of AQP1 under stimulations at different time points was measured by real time RT-PCR. Results AQP1 was immunolocalized predominantly to the microvessels and mesothelial cells of visceral and parietal pleura. The extent of AQP1expression in parietal pleura was less than that in visceral pleura[ ( 4. 14 ±1. 12) ×104 copy /μg vs ( 7. 43 ±2. 02) ×104 copy / μg, P lt;0. 05] . AQP1 expression increased at all phases in the dexamethasone group andthe hypertonic saline group, whereas decreased in the erythromycin group and the lipopolysaccharide group.Conclusion The stimulations of dexamethasone, lipopolysaccharide, erythromycin and hypertonic saline can significantly change the AQP1 expression in pleura, which indicate that AQP1 may contribute to the accumulation and clearance of pleuritic fluids.

Citation： LIAO Huai,XIE Canmao. Expression of Aquaporin-1 in the Pleura in Rats and the Role of Aquaporin-1 in the Pleural Fluid Turnover. Chinese Journal of Respiratory and Critical Care Medicine, 2009, 09(2): 151-155. doi: Copy

1.	Preston GM, Agre P. Isolation of the cDNA for erythrocytes integral membrane protein of 28 kD:Member of an ancient channel family.Proc Natl Acad Sci USA, 1991, 88: 11110-11114.
2.	Verkman AS. Physiological importance of aquaporin water channels.Ann Med, 2002, 34: 192-200.
3.	Agre P. The aquaporin water channels. Proc Am Soc, 2006, 3: 5-13.
4.	King LS, Nielsen S, Agre P. Respiratory Aquaporins in Lung Inflammation. Am J Respir Cell Mol Biol, 2000 , 22: 8 -10 .
5.	Towne EE, Harrod KS. Decreased Expression of Aquaporin( AQP) 1 and AQP5 in Mouse Lung after Acute Viral Infection. Am J Respir Cell Mol Biol, 2000, 22: 34-44.
6.	张伟, 谢灿茂, 黄建强, 等. 高渗液对大鼠胸膜间皮细胞水通道蛋白1 表达的影响. 中华医学杂志, 2007, 87: 2508-2511.
7.	张伟, 谢灿茂. 角叉菜胶致炎症性胸腔积液大鼠胸膜上水通道蛋白1 的表达. 中国呼吸与危重监护杂志, 2008, 7: 190-194.
8.	Yang B, Folkesson HG, Yang J, et al. Reduced osmotic water permeability of the peritoneal barrier in aquaporin-1 knockout mice.Am J Physiol Cell Physiol, 1999, 276: c76-c81.
9.	Moon C, King LS, Agre P. AQP1 expression in erythroleukemia cells: genetic regulation of glucocorticoid and chemical induction.Am J Physiol Cell Physiol, 1997, 273: c1562-c1570.
10.	Jiao G, Li E, Yu R. Decreased expression of AQP1 and AQP5 in acute injured lungs in rats. Chin Med J, 2002, 115: 963-967.
11.	Song Y, Fukuda N, Bai C, et al. Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice. J Physiol, 2000, 525: 771-779.
12.	黄建强, 谢灿茂. 红霉素对人胸膜间皮细胞生长及肿瘤坏死因子-α、转化生长因子-β1 分泌和缝隙连接蛋白43 表达的影响.中华结核和呼吸杂志, 2003, 26: 206-209.
13.	Miserocchi G. Physiology and pathophysiology of pleural fluid turnover. Eur Respir J, 1997, 10: 219-225.
14.	Miserocchi G, Venturoli D, Negrini D, et al.Model of pleural fluid turnover. J Appl Physiol, 1993, 75: 1798-1806.
15.	Wiener-Kronish JP, Albertine KH, Lick V, et al. Protein egress and entry rates in pleural fluid and plasma in sheep. J Appl Physiol, 1984, 56: 459-463.
16.	Lee CH, Wang WJ, Lan RS, et al. Corticosteroids in the treatment of tuberculous pleurisy. A double-blind, placebo-controlled,randomized study. Chest, 1988, 94: 256-1259.
17.	Xie CM, Teixeira LR, McGovern JP, et al. Systemic corticosteroids decrease the effectiveness of talc pleurodesis. AmJ Respir Crit Care Med, 1998, 157: 1441-1444.
18.	Gopi A, Madhavan SM, Sharma SK, et al. Diagnosis and treatment of tuberculous pleural effusion in 2006. Chest, 2007, 131: 880-889.
19.	Sartori C, Matthay MA. Alveolar epithelial fluid transport in acute lung injury: new insights. Eur Respir J, 2002, 20: 1299-1313.
20.	Leitch V, Agre P, King LS. Altered ubiquitination and stability of aquaporin-1 in hypertonic stress. PNAS, 2001, 98: 2894-2898.
21.	Hoffert JD, Agre P, King LS. Hypertonic Induction of Aquaporin-5 Expression through an ERK-dependent Pathway. J Biol Chem,2000, 275: 9070-9077.
22.	Song Y, Matthay MA, Verkman AS. Role of Aquaporin water channels in Pleural fluid dynamics. Am J Physiol Cell Physiol,2000, 279: 1744-1750.
23.	Fang W, Qian J, Yu Z, et al. Expression of aquaporin-1 in the human peritoneum and the effect of peritoneal dialysis on its expression. Chin Med J, 2003, 116: 1370-1373.
24.	谢艳萍, 陈才平, 王建春, 等. 脂多糖、肿瘤坏死因子-α、白细胞介素-1β鼠肺微血管内皮细胞水通道蛋白-1 表达的影响. 中国呼吸与危重监护杂志, 2005, 4: 142-145.

1. Preston GM, Agre P. Isolation of the cDNA for erythrocytes integral membrane protein of 28 kD:Member of an ancient channel family.Proc Natl Acad Sci USA, 1991, 88: 11110-11114.
2. Verkman AS. Physiological importance of aquaporin water channels.Ann Med, 2002, 34: 192-200.
3. Agre P. The aquaporin water channels. Proc Am Soc, 2006, 3: 5-13.
4. King LS, Nielsen S, Agre P. Respiratory Aquaporins in Lung Inflammation. Am J Respir Cell Mol Biol, 2000 , 22: 8 -10 .
5. Towne EE, Harrod KS. Decreased Expression of Aquaporin( AQP) 1 and AQP5 in Mouse Lung after Acute Viral Infection. Am J Respir Cell Mol Biol, 2000, 22: 34-44.
6. 张伟, 谢灿茂, 黄建强, 等. 高渗液对大鼠胸膜间皮细胞水通道蛋白1 表达的影响. 中华医学杂志, 2007, 87: 2508-2511.
7. 张伟, 谢灿茂. 角叉菜胶致炎症性胸腔积液大鼠胸膜上水通道蛋白1 的表达. 中国呼吸与危重监护杂志, 2008, 7: 190-194.
8. Yang B, Folkesson HG, Yang J, et al. Reduced osmotic water permeability of the peritoneal barrier in aquaporin-1 knockout mice.Am J Physiol Cell Physiol, 1999, 276: c76-c81.
9. Moon C, King LS, Agre P. AQP1 expression in erythroleukemia cells: genetic regulation of glucocorticoid and chemical induction.Am J Physiol Cell Physiol, 1997, 273: c1562-c1570.
10. Jiao G, Li E, Yu R. Decreased expression of AQP1 and AQP5 in acute injured lungs in rats. Chin Med J, 2002, 115: 963-967.
11. Song Y, Fukuda N, Bai C, et al. Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice. J Physiol, 2000, 525: 771-779.
12. 黄建强, 谢灿茂. 红霉素对人胸膜间皮细胞生长及肿瘤坏死因子-α、转化生长因子-β1 分泌和缝隙连接蛋白43 表达的影响.中华结核和呼吸杂志, 2003, 26: 206-209.
13. Miserocchi G. Physiology and pathophysiology of pleural fluid turnover. Eur Respir J, 1997, 10: 219-225.
14. Miserocchi G, Venturoli D, Negrini D, et al.Model of pleural fluid turnover. J Appl Physiol, 1993, 75: 1798-1806.
15. Wiener-Kronish JP, Albertine KH, Lick V, et al. Protein egress and entry rates in pleural fluid and plasma in sheep. J Appl Physiol, 1984, 56: 459-463.
16. Lee CH, Wang WJ, Lan RS, et al. Corticosteroids in the treatment of tuberculous pleurisy. A double-blind, placebo-controlled,randomized study. Chest, 1988, 94: 256-1259.
17. Xie CM, Teixeira LR, McGovern JP, et al. Systemic corticosteroids decrease the effectiveness of talc pleurodesis. AmJ Respir Crit Care Med, 1998, 157: 1441-1444.
18. Gopi A, Madhavan SM, Sharma SK, et al. Diagnosis and treatment of tuberculous pleural effusion in 2006. Chest, 2007, 131: 880-889.
19. Sartori C, Matthay MA. Alveolar epithelial fluid transport in acute lung injury: new insights. Eur Respir J, 2002, 20: 1299-1313.
20. Leitch V, Agre P, King LS. Altered ubiquitination and stability of aquaporin-1 in hypertonic stress. PNAS, 2001, 98: 2894-2898.
21. Hoffert JD, Agre P, King LS. Hypertonic Induction of Aquaporin-5 Expression through an ERK-dependent Pathway. J Biol Chem,2000, 275: 9070-9077.
22. Song Y, Matthay MA, Verkman AS. Role of Aquaporin water channels in Pleural fluid dynamics. Am J Physiol Cell Physiol,2000, 279: 1744-1750.
23. Fang W, Qian J, Yu Z, et al. Expression of aquaporin-1 in the human peritoneum and the effect of peritoneal dialysis on its expression. Chin Med J, 2003, 116: 1370-1373.
24. 谢艳萍, 陈才平, 王建春, 等. 脂多糖、肿瘤坏死因子-α、白细胞介素-1β鼠肺微血管内皮细胞水通道蛋白-1 表达的影响. 中国呼吸与危重监护杂志, 2005, 4: 142-145.

Chinese Journal of Respiratory and Critical Care Medicine

Expression of Aquaporin-1 in the Pleura in Rats and the Role of Aquaporin-1 in the Pleural Fluid Turnover

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