The Effect of Cigarette Smoking on Expression of Matrix Metalloproteinase-9 in Airway Epithelium of Rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

JING Xia ,  XU Jianying.

Department of Respiratory Medicine, First Affiliated Hospital of Shanxi Medical University. Taiyuan, Shanxi, 030001, China;

Corresponding author：

XU Jianying., Email: xujyty@tom.com

Keywords：

Chronic obstructive pulmonary disease; Matrix metalloproteinase-9; Smoking; Smoking cessation; Airway remodeling

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Objective To investigate the effects of smoking intensity, duration and cessation on mRNA and protein expressions of matrix metalloproteinase-9 ( MMP-9) in tracheal epitheliumof rats, and the relationship between smoking or smoking cessation and airway remodeling in chronic obstructive pulmonary disease ( COPD) . Methods Forty Wistar rats were randomly divided into 5 groups, ie. a normal control group, a long termheavy smoking group, a short termheavy smoking group, a long termlight smoking group,and a smoking cessation group which was exposed to room air for 10 weeks after long term heavy smoking.The expressions of MMP-9 mRNA and protein in tracheal epithelium of rats were detected by in situ hybridization and munohistochemistry respectively. Results ( 1) The pathological changes of emphysema were observed in the lung tissue of every smoking rat, and were most sever in the long term heavy smoking group. ( 2) Compared with the normal control group [ ( 0. 88 ±0. 88) PU, ( 2. 80 ±1. 66) PU] , the expressions of MMP-9 mRNA and proteins in tracheal epithelium were remarkable elevated in the long term heavy smoking group [ ( 22. 01 ±2. 86) PU, ( 20. 81 ±2. 46) PU] , the short term heavy smoking group [ ( 14. 94 ±3. 46) PU, ( 13. 68 ±2. 00) PU] , the long term light smoking group [ ( 6. 92 ±2. 71) PU,( 8. 84 ±1. 80) PU] and the smoking cessation group [ ( 19. 00 ±3. 36) PU, ( 14. 82 ±1. 74) PU] ( P lt;0. 01) . Compared with the long term heavy smoking group, the expressions of MMP-9 in tracheal epithelium were decreased in other three smoking groups ( P lt; 0. 05) . Conclusions Smoking could increase the expression of MMP-9 in tracheal epithelium and cause trachea damage and remodeling with intensity and duration in rats. Smoking cessation could decrease the MMP-9 expression and alleviate trachea remodeling,suggesting its role in the prevention of COPD.

Citation： JING Xia,XU Jianying.. The Effect of Cigarette Smoking on Expression of Matrix Metalloproteinase-9 in Airway Epithelium of Rats. Chinese Journal of Respiratory and Critical Care Medicine, 2009, 09(3): 220-223. doi: Copy

1.	Ward SA, Casaburi R.
2.	陈京霞, 李淑兰. 基质金属蛋白酶-9 与呼吸道重塑. 医学综述,2006, 12: 1353-1355.
3.	许三林, 吴人亮, 陈春莲, 等. 上皮钙粘素在吸烟小鼠呼吸道上皮损伤修复中表达的研究. 中华结核和呼吸杂志, 1999, 22: 417-419.
4.	姚婉贞, 陈亚红.
5.	Kang MJ, Oh YM, Lee JC, et al. Lung matrix metalloproteinase-9 correlates with cigarette smoking and obstruction of airflow. J Korean Med Sci, 2003, 18: 821-827.
6.	刘先胜, 徐永健. 慢性阻塞性肺疾病发病机制的研究进展. 中国呼吸与危重监护杂志, 2004, 3: 70-72.
7.	周玉民, 冉丕鑫. 慢性阻塞性肺疾病的流行病学. 中国呼吸与危重监护杂志, 2004, 3: 68-70.
8.	Vignola AM, Riccobono L, Mirabella A, et al. Sputum metalloproteinase-9 / tissue inhibitor of metalloproteinase-1 ratio correlates with airflow obstruction in asthma and chronic bronchitis. Am J Respir Crit Care Med, 1998, 158: 1945-1950.
9.	张培芳, 辛建保, 潘磊. MMP-9 和TIMP-1 在大鼠肺气肿模型细胞外基质重塑中的作用. 中国医师杂志, 2003, 5: 306-308.
10.	Doherty DE. Early detection and management of COPD. What you can do to reduce the impact of this disabling disease. Postgrad Med,2002, 111: 41-53.
11.	st century perspective on chronic obstructive pulmonary disease. Respiration, 2001, 68: 557-561.
12.	年欧洲呼吸学会年会慢性阻塞性肺疾病研究进展. 中国呼吸与危重监护杂志, 2004, 3: 413-415.

1. Ward SA, Casaburi R.
2. 陈京霞, 李淑兰. 基质金属蛋白酶-9 与呼吸道重塑. 医学综述,2006, 12: 1353-1355.
3. 许三林, 吴人亮, 陈春莲, 等. 上皮钙粘素在吸烟小鼠呼吸道上皮损伤修复中表达的研究. 中华结核和呼吸杂志, 1999, 22: 417-419.
4. 姚婉贞, 陈亚红.
5. Kang MJ, Oh YM, Lee JC, et al. Lung matrix metalloproteinase-9 correlates with cigarette smoking and obstruction of airflow. J Korean Med Sci, 2003, 18: 821-827.
6. 刘先胜, 徐永健. 慢性阻塞性肺疾病发病机制的研究进展. 中国呼吸与危重监护杂志, 2004, 3: 70-72.
7. 周玉民, 冉丕鑫. 慢性阻塞性肺疾病的流行病学. 中国呼吸与危重监护杂志, 2004, 3: 68-70.
8. Vignola AM, Riccobono L, Mirabella A, et al. Sputum metalloproteinase-9 / tissue inhibitor of metalloproteinase-1 ratio correlates with airflow obstruction in asthma and chronic bronchitis. Am J Respir Crit Care Med, 1998, 158: 1945-1950.
9. 张培芳, 辛建保, 潘磊. MMP-9 和TIMP-1 在大鼠肺气肿模型细胞外基质重塑中的作用. 中国医师杂志, 2003, 5: 306-308.
10. Doherty DE. Early detection and management of COPD. What you can do to reduce the impact of this disabling disease. Postgrad Med,2002, 111: 41-53.
11. st century perspective on chronic obstructive pulmonary disease. Respiration, 2001, 68: 557-561.
12. 年欧洲呼吸学会年会慢性阻塞性肺疾病研究进展. 中国呼吸与危重监护杂志, 2004, 3: 413-415.

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