Effect of Glucose on Expression of Aquaporin-1 in Pleural Mesothelial Cells in Rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

ZHANG Wei ¹ , LIU Daoming ¹ , MAO Qiuyun ¹ ,  XIE Canmao. ²

Department of Respiratory Medicine, The Central Hospital of Taian. Taian, Shandong, 271000, China;

Corresponding author：

XIE Canmao., Email: xiecanmao@163.com

Keywords：

Glucose; Pleural mesothelial cells; Aquaporin-1; Pleural effusion

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Objective To investigate the expression of aquaporin-1( AQP-1) in pleural mesothelial cells ( PMCs) and the influence of glucose thereupon. Methods Rat PMCs were isolated, cultured, and divided into two groups, ie. a glucose group, cultured with glucose of different concentrations for 24 hours,and a control group, cultured in D-MEM/ F-12 medium. The 100 mmol / L glucose group was administered at the time points of 6, 12, 18, and 24 hours respectively. RT-PCR and Western blotting were used to analyze the mRNA and protein expression of AQP-1. Results The absorbance values of AQP-1 protein expression were 54. 02 ±4. 61, 127. 84 ±9. 41, and 231. 62 ±22. 63, respectively in the PMCs treated with glucose of the concentrations of 50, 100, and 200 mmol / L, all significantly higher than those in the control group( 22. 45 ±2. 16, all P lt; 0. 01) . The absorbance values of AQP-1 protein expression were 24. 68 ±2. 56, 58. 68 ±3. 67, 89. 61 ±6. 62, and 113. 41 ±7. 65 in the PMCs treated with glucose of the concentration of 100 mmol / L after 6, 12, 18, and 24 hours, all significantly higher than those in the control group ( 11. 81 ±1. 45, P lt;0. 01) .Conclusions Glucose induces the expression of AQP-1 mRNA and protein. AQP-1 participates in the pleural fluid formation.

Citation： ZHANG Wei,LIU Daoming,MAO Qiuyun,XIE Canmao.. Effect of Glucose on Expression of Aquaporin-1 in Pleural Mesothelial Cells in Rats. Chinese Journal of Respiratory and Critical Care Medicine, 2009, 09(3): 251-255. doi: Copy

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11.	Umenishi F, Schrier RW. Identification and characterization of a novel hypertonicity-responsive element in the human aquaporin-1 gene. Biochem Biophys Res Commun, 2002, 292: 771-775.
12.	Asai M, Higuchi S, Kubota M, et al. Regulators for blood glucose level affect gene expression of aquaporin 3. Biol Pharm Bull, 2006 ,29: 991-996.

1. Light RW, Lee YCG. Textbook of Pleural Diseases. First ed. Oxford University Press Inc , London, 2003. xiii.
2. Agre P, Kozono D. Aquaporin water channels: molecular mechanisms for human diseases. FEBS Lett, 2003, 555: 72-78.
3. Song Y, Yang B, Matthay MA, et al. Role of aquaporin water channels in pleural fluid dynamics. Am J Physiol Cell Physiol,2000, 279: 1744-1750.
4. 张伟, 谢灿茂. 角叉菜胶致炎症性胸腔积液大鼠胸膜上水通道蛋白1 的表达. 中国呼吸与危重监护杂志, 2008, 7: 190-194.
5. Agre P. The aquaporin water channels. Proc Am Thorac Soc, 2006 ,3: 5-13.
6. Bai C, Fukuda N, Song Y, et al. Lung fluid transport in aquaporin-1 and aquaporin-4 knockout mice. J Clin Invest, 1999, 103: 555-561.
7. King LS, Nielsen S, Agre P. Respiratory aquaporins in lung inflammation: the night is young. Am J Respir Cell Mol Biol, 2000 ,22: 8-10.
8. Lai KN, Li FK, Lan HY, et al. Expression of aquaporin-1 in human peritoneal mesothelial cells and its upregulation by glucose in vitro.J Am Soc Nephrol, 2001, 12: 1036-1045.
9. Lai KN, Leung JC, Chan LY, et al. Expression of aquaporin-3 in human peritoneal mesothelial cells and its up-regulation by glucose in vitro. Kidney Int, 2002, 62: 1431-1439.
10. Leitch V, Agre P, King LS. Altered ubiquitination and stability of aquaporin-1 in hypertonic stress. Proc Natl Acad Sci U S A, 2001 ,98: 2894-2898.
11. Umenishi F, Schrier RW. Identification and characterization of a novel hypertonicity-responsive element in the human aquaporin-1 gene. Biochem Biophys Res Commun, 2002, 292: 771-775.
12. Asai M, Higuchi S, Kubota M, et al. Regulators for blood glucose level affect gene expression of aquaporin 3. Biol Pharm Bull, 2006 ,29: 991-996.

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Effect of Glucose on Expression of Aquaporin-1 in Pleural Mesothelial Cells in Rats

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