The Ubiquitin-Proteasome Pathway Is Activated in the Diaphragm of COPD Rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

HAN Fengfeng , XU Weiguo , LUO Yong , YANG Tianyun , CHEN Yingchun , ZHANG Yue.

Departmant of Respiratory Medicine, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University. Shanghai, 200092, ChinaCorresponding Author: XU Wei-guo, E-mail: xwg_xinhua@ yahoo. com;

Corresponding author：

Keywords：

Chronic obstructive pulmonary disease; Diaphragm; Ubiquitin-proteasome pathway

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Objective To study the role of ubiquitin-proteasome pathway in diaphragm of COPD rats. Mathods Thirty rats were divided into a normal control group and a COPD group. COPD model was established by exposure to cigarette smoke for three months. The protein levels of E2-14k and proteasome subunit C8 in diaphragms were measured by Western blot. The mRNA levels of ubiquitin and proteasome subunit C2 in diaphragms were measured bymeans of realtime polymerase chain reaction( RT-PCR) . Results Compared with the control group, the protein expression of E2-14k increased significantly in the COPD group ( 0. 81 ±0. 28 vs 0. 50 ±0. 25, P lt;0. 05) , but C8 protein level was not significantly different between the two groups( P gt;0. 05) . The mRNA expression of ubiquitin increased significantly in the COPD group( 0. 89 ±0. 20 vs 0. 50 ±0. 15, P lt;0. 05) , but C2 mRNA level was not significantly different between the two groups ( P gt; 0. 05 ) . Conclusions The mRNA and protein expressions of ubiquitin-proteasome pathway in diaphragmincreased significantly in COPD rats, suggesting that the activity of ubiquitin-proteasome pathway
increased, which lead to an increase of protein degradation.

Citation： HAN Fengfeng,XU Weiguo,LUO Yong,YANG Tianyun,CHEN Yingchun,ZHANG Yue.. The Ubiquitin-Proteasome Pathway Is Activated in the Diaphragm of COPD Rats. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(2): 141-144. doi: Copy

1.	王增礼. 更新对慢性阻塞性肺疾病认识的观念. 中国呼吸与危重监护杂志, 2007 , 6: 401-402 .
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12.	Levine S, Nguyen T, Kaiser LR, et al. Human diaphragm remodeling associated with chronic obstructive pulmonary disease: clinical implications. Am J Respir Crit Care Med, 2003, 168: 706 -713.
13.	孙丽华, 欧阳晓平, 谷伟, 等. 胰岛素样生长因子对COPD 大鼠膈肌凋亡的保护作用及对肺功能的影响. 中国呼吸与危重监护杂志, 2009 , 8: 441-445 .
14.	杨天芸, 徐卫国, 罗勇, 等. 慢性阻塞性肺病大鼠骨骼肌蛋白降解的变化. 上海交通大学学报( 医学版) , 2008, 28 : 525-527.
15.	韩锋锋, 罗勇, 徐卫国. 慢性阻塞性肺疾病大鼠骨骼肌组织泛素系统基因及蛋白表达变化的研究. 上海医学, 2008; 31: 571 -574.
16.	Taillandier D, Combaret L, Pouch MN, et al. The role of ubiquitinproteasome-dependent proteolysis in the remodelling of skeletal muscle. Proc Nutr Soc, 2004, 63 : 357-361.
17.	McKinnell IW, Rudnicki MA. Molecular mechanisms of muscle atrophy. Cell, 2004, 119: 907-910.
18.	Kornitzer D, Ciechanover A. Modes of regulation of ubiquitinmediated protein degradation. J Cell Physiol, 2000 , 182: 1-11.

1. 王增礼. 更新对慢性阻塞性肺疾病认识的观念. 中国呼吸与危重监护杂志, 2007 , 6: 401-402 .
2. Wray CJ, Sun X, Gang GJ, et al. Dantrolene downregulates the gene expression and activity of the ubiquitin-proteasome proteolytic pathway in septic skeletal muscle. J Surg Res, 2002 , 104: 82-87 .
3. 李红梅, 崔德健, 佟欣, 等. 熏香烟加气管注内毒素和单纯熏香烟法建立大鼠COPD 模型. 中国病理生理杂志, 2002, 18: 808-812.
4. Ausubel MF, Brent R, Konston ER, et al. 马学军, 舒跃龙, 译校. 精编分子生物学实验指南. 第4 版. 北京: 科学出版社,2005,378-416.
5. Ezzell L, Jensen GI. Malnutrition in chronic obstructive pulmonary disease. Am J Clin Nutr, 2000, 72: 1415-1416.
6. Laaban JP. Nutrition and chronic respiratory failure. Ann Inter Med,2000, 151: 542-548.
7. Jagoe RT, Engelen MP. Muscle wasting and changes in muscle protein metabolism in chronic obstructive pulmonary disease. Eur Respir J, 2003, 46 : 52s-63 s.
8. Rutten EP, Franssen FM, Engelen MP, et al. Greater whole-body myofibrillar protein breakdown incachectic patients with chronic obstructive pulmonary disease. AmJ Clin Nutr,2006,83( 4) :829-834.
9. Laghi F, Tobin MJ. Disorders of the respiratory muscles. Am J Respir Crit Care Med, 2003, 168: 10 -48.
10. Ottenheijm CAC, Heunks LMA, Hafmans T, et al. Titin and diaphragmdysfunction in chronic obstructive pulmonary disease. Am J Respir Crit Care Med, 2006 , 173: 527-534 .
11. Barreiro E, de la Puente B, Minguella J, et al. Oxidative stress and respiratory muscle dysfunction in severe chronic obstructive pulmonary disease. Am J Respir Crit Care Med,2005, 171:1116-1124.
12. Levine S, Nguyen T, Kaiser LR, et al. Human diaphragm remodeling associated with chronic obstructive pulmonary disease: clinical implications. Am J Respir Crit Care Med, 2003, 168: 706 -713.
13. 孙丽华, 欧阳晓平, 谷伟, 等. 胰岛素样生长因子对COPD 大鼠膈肌凋亡的保护作用及对肺功能的影响. 中国呼吸与危重监护杂志, 2009 , 8: 441-445 .
14. 杨天芸, 徐卫国, 罗勇, 等. 慢性阻塞性肺病大鼠骨骼肌蛋白降解的变化. 上海交通大学学报( 医学版) , 2008, 28 : 525-527.
15. 韩锋锋, 罗勇, 徐卫国. 慢性阻塞性肺疾病大鼠骨骼肌组织泛素系统基因及蛋白表达变化的研究. 上海医学, 2008; 31: 571 -574.
16. Taillandier D, Combaret L, Pouch MN, et al. The role of ubiquitinproteasome-dependent proteolysis in the remodelling of skeletal muscle. Proc Nutr Soc, 2004, 63 : 357-361.
17. McKinnell IW, Rudnicki MA. Molecular mechanisms of muscle atrophy. Cell, 2004, 119: 907-910.
18. Kornitzer D, Ciechanover A. Modes of regulation of ubiquitinmediated protein degradation. J Cell Physiol, 2000 , 182: 1-11.

Chinese Journal of Respiratory and Critical Care Medicine

The Ubiquitin-Proteasome Pathway Is Activated in the Diaphragm of COPD Rats

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