The Comparison of Vasoactive Effects of Norepinephrine and Dopamine on Isolated Rabbit Pulmonary and Systemic Arteries by LPS Pre-incubation_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

TONG Dongyi ¹ , ZHANG Jin ¹ , YU Hongbo ² , LI Na ³ , WEI Huixia. ¹

Department of Anesthesiology, Shengjing Hospital, China Medical University. Shenyang,Liaoning,110004, ChinaCorresponding Author: ZHANG Jin, E-mail: zhangj@ sj-hospital. org;

Corresponding author：

Keywords：

Endotoxin; Shock; Sepsis; Pulmonary artery; Systemic artery; Norepinephrine; Dopamine

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Objective To compare the vasoactive effects of norepinephrine( NE) and dopamine of different doses on isolated rabbit pulmonary and systemic arteries in septic shock. Methods Six paired pulmonary and systemic arterial rings were prepared fromsix rabbits, and matched randomly assigned into a normal group and a LPS group. The assigned groups were intervened by different doses of NE. Another six paired pulmonary and systemic arterial rings were prepared from another six rabbits. They were assigned to different groups as above and intervened by different doses of dopamine. The LPS groups were pre-incubated in RPMI mediumsupplemented with4 μg/mL LPS to simulate septic shock. The tension of arterial rings was measured and its response to NE and dopamine were studied. Results ( 1) In the normal groups, the contraction of the systemic arteries was ber than the pulmonary arteries in response to low,middle dose of NE, and high dose of dopamine ( all P lt; 0. 05) , and which was weaker in response to middle dose of dopamine and similar in response to high dose of NE( P gt;0. 05) . Both the pulmonary and systemic arteries
relaxed in response to low dose of dopamine. ( 2) After LPS pre-incubation, the contraction of the systemic arteries was weaker than the pulmonary arteries in response to low dose of dopamine ( P lt;0. 05) , and which was similar in response to low,middle and high dose of NE, and middle, high dose of dopamine. ( 3) Comparing the LPS groups with the normal groups, the contraction in response to middle dose of dopamine increased in the systemic arteries and dreased in the pulmonary arteries ( P lt;0. 05) . Conclusions In septic shock, the vasoactive effect of different doses of NE is not different between pulmonary and systemic arteries. But middle dose of dopamine can increase the contraction of systemic arteries and decrease the contraction of pulmonary arteries.

Citation： TONG Dongyi,ZHANG Jin,YU Hongbo,LI Na,WEI Huixia.. The Comparison of Vasoactive Effects of Norepinephrine and Dopamine on Isolated Rabbit Pulmonary and Systemic Arteries by LPS Pre-incubation. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(2): 188-191. doi: Copy

1.	Varpula M, Tallgren M, Saukkonen K, et al. Hemodynamic variables related to outcome in septic shock. Intensive Care Med, 2005, 31 :1066-1071.
2.	Stevens T, Morris K, McMurtry IF, et al. Pulmonary and systemic vascular responsiveness to TNF-alpha in conscious rats. J Appl Physiol, 1993 , 74: 1905-1910.
3.	Martin C, Viviand X, Leone M, et al. Effect of norepinephrine on the outcome of septic shock. Crit Care Med, 2000, 28: 2758-2765.
4.	Dellinger RP, Levy MM, Carlet JM, et al. Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock: 2008 . Intensive Care Med, 2008, 34: 17-60.
5.	谷振勇, 凌亦凌, 孟爱宏, 等. 八肽胆囊收缩素对脂多糖诱导离体兔肺动脉反应性变化的影响. 中国病理生理杂志, 1999, 15 :484 -486.
6.	Huang XL, Ling YL, Zhu TN, et al. The role of N-acetylcysteine against the injury of pulmonary artery induced by LPS. Chin J Appl Physiol, 2002 , 18: 370 -372.
7.	邢邯英, 凌亦凌, 孟爱宏, 等. 褪黑素改善内毒素血症大鼠血管反应性. 生理学校, 2005, 57: 367-372.
8.	Lee TS, Hou X. Vasoactive effects of ketamine on isolated rabbit pulmonary arteries. Chest, 1995 , 107: 1152-1155.
9.	徐叔云, 主编. 药理实验方法学, 第3 版. 北京: 人民卫生出版社, 2002, 1535.
10.	朱绿绮. 感染性休克血管活性药物的应用. 小儿急救医学,2004, 11: 77-78 .
11.	索良源, 张锦. 去甲肾上腺素治疗感染性休克时对肺静脉血管阻力的影响. 中国呼吸与危重监护杂志, 2009 , 8: 303-306 .
12.	赵悦, 王雪莲, 刘沛, 等. 内毒素休克大鼠胸主动脉平滑肌细胞超微结构改变. 中国医科大学校报, 2006, 35: 3-4.
13.	刘良明, 胡德耀, 陈惠孙, 等. 循环休克肾上腺素能受体失敏研究进展. 中国病理生理杂志, 1998, 14 : 100-103.
14.	Kirkeb en KA, Strand OA. The role of nitric oxide in sepsis--an overview. Acta Anaesthesiol Scand, 1999, 43 : 275-288.
15.	Sheridan BC, McIntyre RC Jr, Moore EE, et al. Neutrophils mediate pulmonary vasomotor dysfunction in endotoxin-induced acute lung injury. J Trauma, 1997, 42 : 391-396 .
16.	Frostermann U, Pollock JS, Nakane M, et al. Noitric oxide synthases in the cardiovascular system. Trends Cardiovasc Med, 1993, 3 : 104 -110 .
17.	段国辰, 凌亦凌, 谷振勇, 等. 八肽胆囊收缩素对家兔内毒素休克时主动脉及肺动脉反应性改变的影响. 中国急救医学, 2002 ,22: 693-697.
18.	Atkinson J, Trescases N, Benedek C, et al. Alpha-1 and alpha-2 adrenoceptor agonists induce vasoconstriction of the normotensive ratcaudal artery in vitro by stimulation of a heterogeneous population of alpha-1 adrenoceptors. Naunyn Schmiedebergs Arch Pharmacol,1988, 338 : 529-535.
19.	Luke J. Janssen, Hwa Lu-chao, et al. Netherton. Excitationcontraction coupling in pulmonary vascular smooth muscle involves tyrosine kinase and Rho kinase. Am J Physiol Lung Cell Mol Physiol, 2001 , 280: 666-674.
20.	陈玮, 明佳, 徐竞, 等. 内毒素休克后家兔血管反应性的变化规律及器官差异. 创伤外科杂志, 2008, 10: 456-459 .
21.	Hasan A, McDonough KH. The effects of Escherichia coli sepsis and short-term ischemia on coronary vascular reactivity and myocardial function. Shock, 1997, 8: 305 -310.
22.	Emmanuele DI, Villa Bianca R, Lippolis L, et al. Dexamethasone improves vascular hyporeactivity induced by lps in vivo by modulating ATP-sensitive potassium channels activity. Br J Pharmacol, 2003 , 140: 91-96 .
23.	Boffa JJ, Arendshorst WJ. Maintenance of renal vascular reactivity contributes to acute renal failure during endotoxemic shock. J Am Soc Nephrol, 2005, 16: 117-124.
24.	Takakura K, Xiaohong W, Takeuchi K, et al. Deactivation of norepinephrine by peroxynitrite as a new pathogenesis in the hypotension of septic shock. Anesthesiology, 2003 , 98: 928-934.
25.	Polak MJ, Drummond WH. Systemic and pulmonary vascular effects of selective dopamine receptor blockade and stimulation in lambs.Pediatr Res, 1993 , 33: 181 -184.
26.	Velasco M, Luchsinger A. Dopamine: pharmacologic and therapeutic aspects. Am J Ther, 1998, 5: 37-43.
27.	朱琳, 赵荣瑞, 张玮芳, 等. 多巴胺受体激动剂对兔动脉cAMP产生系统的影响. 生理学报, 2000 , 52 : 247-251.
28.	Riui A, Collier WI, Amentu F, et al. Endothelial Dopamine DA1 recep torsites in the rabbit pulmonary artery: autoradiographic demonstraction. Journal of harmacalogy & ecperimental Therapeutics, 2003, 261: 830-834.
29.	朱琳, 刘爱华, 赵荣瑞, 等. DA1 受体激动剂对大鼠离体肺动脉和肠动脉cAMP 生成量的影响. 河南医科大学学报, 2001, 36 :249-251.
30.	支建明, 郭薇, 张炜芳, 等. 肺动脉高压大鼠外周血管多巴胺-1型受体反应性的变化. 中国病理生理杂志, 1998, 14: 692-695 .
31.	曹惠芳, 徐凤珍, 刘超, 等. 血浆内皮衍生性舒缩因子对急性肺损伤患者肺循环动力学及呼吸力学的影响. 中国呼吸与危重监护杂志, 2003, 2: 87-90.
32.	PENG HL, JIANG MH, YANG ZC, et al. Determination of betaadrenoceptor subtypes in human pulmonary artery andthoracic aorta by radiolig and binding. Acta Pharmacotoglca Sinica, 1993, 14: 298 -301.
33.	王节发, 薛全福, 刘敬忠, 等. PCR 技术研究缺氧时大鼠肺肾上腺素能β2 受体及其mRNA 的变化. 中国病理生理杂志, 1995 ,11: 6-10.

1. Varpula M, Tallgren M, Saukkonen K, et al. Hemodynamic variables related to outcome in septic shock. Intensive Care Med, 2005, 31 :1066-1071.
2. Stevens T, Morris K, McMurtry IF, et al. Pulmonary and systemic vascular responsiveness to TNF-alpha in conscious rats. J Appl Physiol, 1993 , 74: 1905-1910.
3. Martin C, Viviand X, Leone M, et al. Effect of norepinephrine on the outcome of septic shock. Crit Care Med, 2000, 28: 2758-2765.
4. Dellinger RP, Levy MM, Carlet JM, et al. Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock: 2008 . Intensive Care Med, 2008, 34: 17-60.
5. 谷振勇, 凌亦凌, 孟爱宏, 等. 八肽胆囊收缩素对脂多糖诱导离体兔肺动脉反应性变化的影响. 中国病理生理杂志, 1999, 15 :484 -486.
6. Huang XL, Ling YL, Zhu TN, et al. The role of N-acetylcysteine against the injury of pulmonary artery induced by LPS. Chin J Appl Physiol, 2002 , 18: 370 -372.
7. 邢邯英, 凌亦凌, 孟爱宏, 等. 褪黑素改善内毒素血症大鼠血管反应性. 生理学校, 2005, 57: 367-372.
8. Lee TS, Hou X. Vasoactive effects of ketamine on isolated rabbit pulmonary arteries. Chest, 1995 , 107: 1152-1155.
9. 徐叔云, 主编. 药理实验方法学, 第3 版. 北京: 人民卫生出版社, 2002, 1535.
10. 朱绿绮. 感染性休克血管活性药物的应用. 小儿急救医学,2004, 11: 77-78 .
11. 索良源, 张锦. 去甲肾上腺素治疗感染性休克时对肺静脉血管阻力的影响. 中国呼吸与危重监护杂志, 2009 , 8: 303-306 .
12. 赵悦, 王雪莲, 刘沛, 等. 内毒素休克大鼠胸主动脉平滑肌细胞超微结构改变. 中国医科大学校报, 2006, 35: 3-4.
13. 刘良明, 胡德耀, 陈惠孙, 等. 循环休克肾上腺素能受体失敏研究进展. 中国病理生理杂志, 1998, 14 : 100-103.
14. Kirkeb en KA, Strand OA. The role of nitric oxide in sepsis--an overview. Acta Anaesthesiol Scand, 1999, 43 : 275-288.
15. Sheridan BC, McIntyre RC Jr, Moore EE, et al. Neutrophils mediate pulmonary vasomotor dysfunction in endotoxin-induced acute lung injury. J Trauma, 1997, 42 : 391-396 .
16. Frostermann U, Pollock JS, Nakane M, et al. Noitric oxide synthases in the cardiovascular system. Trends Cardiovasc Med, 1993, 3 : 104 -110 .
17. 段国辰, 凌亦凌, 谷振勇, 等. 八肽胆囊收缩素对家兔内毒素休克时主动脉及肺动脉反应性改变的影响. 中国急救医学, 2002 ,22: 693-697.
18. Atkinson J, Trescases N, Benedek C, et al. Alpha-1 and alpha-2 adrenoceptor agonists induce vasoconstriction of the normotensive ratcaudal artery in vitro by stimulation of a heterogeneous population of alpha-1 adrenoceptors. Naunyn Schmiedebergs Arch Pharmacol,1988, 338 : 529-535.
19. Luke J. Janssen, Hwa Lu-chao, et al. Netherton. Excitationcontraction coupling in pulmonary vascular smooth muscle involves tyrosine kinase and Rho kinase. Am J Physiol Lung Cell Mol Physiol, 2001 , 280: 666-674.
20. 陈玮, 明佳, 徐竞, 等. 内毒素休克后家兔血管反应性的变化规律及器官差异. 创伤外科杂志, 2008, 10: 456-459 .
21. Hasan A, McDonough KH. The effects of Escherichia coli sepsis and short-term ischemia on coronary vascular reactivity and myocardial function. Shock, 1997, 8: 305 -310.
22. Emmanuele DI, Villa Bianca R, Lippolis L, et al. Dexamethasone improves vascular hyporeactivity induced by lps in vivo by modulating ATP-sensitive potassium channels activity. Br J Pharmacol, 2003 , 140: 91-96 .
23. Boffa JJ, Arendshorst WJ. Maintenance of renal vascular reactivity contributes to acute renal failure during endotoxemic shock. J Am Soc Nephrol, 2005, 16: 117-124.
24. Takakura K, Xiaohong W, Takeuchi K, et al. Deactivation of norepinephrine by peroxynitrite as a new pathogenesis in the hypotension of septic shock. Anesthesiology, 2003 , 98: 928-934.
25. Polak MJ, Drummond WH. Systemic and pulmonary vascular effects of selective dopamine receptor blockade and stimulation in lambs.Pediatr Res, 1993 , 33: 181 -184.
26. Velasco M, Luchsinger A. Dopamine: pharmacologic and therapeutic aspects. Am J Ther, 1998, 5: 37-43.
27. 朱琳, 赵荣瑞, 张玮芳, 等. 多巴胺受体激动剂对兔动脉cAMP产生系统的影响. 生理学报, 2000 , 52 : 247-251.
28. Riui A, Collier WI, Amentu F, et al. Endothelial Dopamine DA1 recep torsites in the rabbit pulmonary artery: autoradiographic demonstraction. Journal of harmacalogy & ecperimental Therapeutics, 2003, 261: 830-834.
29. 朱琳, 刘爱华, 赵荣瑞, 等. DA1 受体激动剂对大鼠离体肺动脉和肠动脉cAMP 生成量的影响. 河南医科大学学报, 2001, 36 :249-251.
30. 支建明, 郭薇, 张炜芳, 等. 肺动脉高压大鼠外周血管多巴胺-1型受体反应性的变化. 中国病理生理杂志, 1998, 14: 692-695 .
31. 曹惠芳, 徐凤珍, 刘超, 等. 血浆内皮衍生性舒缩因子对急性肺损伤患者肺循环动力学及呼吸力学的影响. 中国呼吸与危重监护杂志, 2003, 2: 87-90.
32. PENG HL, JIANG MH, YANG ZC, et al. Determination of betaadrenoceptor subtypes in human pulmonary artery andthoracic aorta by radiolig and binding. Acta Pharmacotoglca Sinica, 1993, 14: 298 -301.
33. 王节发, 薛全福, 刘敬忠, 等. PCR 技术研究缺氧时大鼠肺肾上腺素能β2 受体及其mRNA 的变化. 中国病理生理杂志, 1995 ,11: 6-10.

Chinese Journal of Respiratory and Critical Care Medicine

The Comparison of Vasoactive Effects of Norepinephrine and Dopamine on Isolated Rabbit Pulmonary and Systemic Arteries by LPS Pre-incubation

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