Effects of Smoking on SerumLeptin,Adiponectin, IL-6, and C-Reactive Protein in Rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

BAI Xueli , XU Jianying

Department of Respiratory Medicine, The First Affiliated Hospital of Shanxi Medical University.Taiyuan, Shanxi, 030001, ChinaCorresponding Author: XU Jian-ying, E-mail: xujyty@ tom. com;

Corresponding author：

Keywords：

Chronic obstructive pulmonary disease; Smoking; Leptin; Adiponectin; Systemic inflammatory response

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Objective To investigate the role of inflammatory factors like serumleptin, adiponectin,interleukin-6( IL-6) , and C-reactive protein ( CRP) in the systemic inflammatory response of smokinginduced COPD. Methods Thirty male Wistar rats were randomly divided into three groups, ie. a high-dose smoking group, a low-dose smoking group, and a control group. Serum leptin, adiponectin, IL-6, and CRP levels were measured by ABC-ELISA. Results The serum leptin and adiponectin levels in both smoking groups decreased significantly compared with the control group( P lt; 0. 05) , while the difference was not significant between the two smoking groups ( P gt; 0. 05) . The serum IL-6 and CRP levels in both smoking groups increased significantly compared with the control group( P lt; 0. 05) , which were higher in the highdose
smoking group than those in the low-dose smoking group( P lt;0. 05) . Conclusions Smoking increases the serum levels of IL-6 and CRP, but reduces the serum levels of leptin and adiponectin in rats. These results suggest that leptin, adiponectin, IL-6, and CRP may be involved in the systemic inflammatory response of smoking-induced COPD.

Citation： BAI Xueli,XU Jianying. Effects of Smoking on SerumLeptin,Adiponectin, IL-6, and C-Reactive Protein in Rats. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(3): 298-300. doi: Copy

1.	van Weel C, Schellevis FG. Comorbidity and guidelines: conflicting interests. Lancet, 2006 , 367: 550-551.
2.	Sin DD, Anthonisen NR, Soriano JB, et al. Mortality in COPD: Role of comorbidities. Eur Respir J, 2006, 28: 1245-1257.
3.	Schols AM, Creutzerg EC, Buurman WA, et al. Plasma leptin is related to proinflammatory status and dietary intake in patients with chronic obstructive pulmonary disease. Am J Respir Crit Care Med,1999, 160: 1220-1226.
4.	Kirdar S, Serter M, Ceylan E, et al. Adiponectin as a biomarker of systemic inflammatory response in smoker patients with stable and exacerbation phases of chronic obstructive pulmonary disease. Scand.J Clin Lab Invest, 2009, 69: 219 -224.
5.	许三林, 吴人亮, 陈春莲, 等. 上皮钙黏附素在吸烟小鼠呼吸道上皮损伤修复中表达的研究. 中华结核和呼吸杂志,1999, 22:417-419.
6.	Chen H, Hansen MJ, Jones JE, et al. Long-term cigarette smoke exposure increases uncoupling protein expression but reduces energy intake. Brain Res, 2008, 1228: 81-88.
7.	Hotta Y, Yatsuya H, Toyoshima H, et al. Low leptin but high insulin resistance of smokers in Japanese men. Diabetes Res Clin Pract,2008, 81: 358-364 .
8.	Sull JW, Kim HJ, Yun JE, et al. Serumadiponectin is associated with smoking status in healthy Korean men. Endocr J, 2009, 56: 73-78.
9.	Kirchgessner TG, Uysal KT, Wiesbrock SM, et al. Tumor necrosis factor-alpha contributes to obesity-related hyperleptinemia by regulating leptin release from adipocytes. J Clin Invest, 1997, 100 :2777-2782.
10.	Trujillo ME, Sullivan S, Harten I, et al. Interleukin-6 regulates human adipose tissue lipid metabolism and leptin production in vitro. J Clin Endocrinol Metab, 2004, 89 : 5577 -5582 .
11.	Fantuzzi G. Adipose tissue, adipokines, and inflammation. J Allergy Clin Immunol, 2005, 115: 911 -919.
12.	Gettys TW, Harkness PJ, Waston PM. The β3-aderenergic receptor inhibits insulin-stinulated leptin secretion from isolated rat adipocytes. Endocrinology, 1996, 137: 4054-4057.
13.	Bruun JM, Lihn AS, Verdich C, et al. Regulation of adiponectin by adipose tissue-derived cytokines: in vivo and in vitro investigations in humans. Am J Physiol Endocrinol Metab, 2003, 285 : E527-533.
14.	Iwashima Y, Katauya T, Ishikawa K, et al. Association of hypoadiponectinemia with smoking habit in men. Hypertension,2005, 45: 1094-1100 .
15.	Pepys MB, Hirschfield GM. C-reactive protein: a critical update. J Clin Invest, 2003 , 111: 1805-1812.

1. van Weel C, Schellevis FG. Comorbidity and guidelines: conflicting interests. Lancet, 2006 , 367: 550-551.
2. Sin DD, Anthonisen NR, Soriano JB, et al. Mortality in COPD: Role of comorbidities. Eur Respir J, 2006, 28: 1245-1257.
3. Schols AM, Creutzerg EC, Buurman WA, et al. Plasma leptin is related to proinflammatory status and dietary intake in patients with chronic obstructive pulmonary disease. Am J Respir Crit Care Med,1999, 160: 1220-1226.
4. Kirdar S, Serter M, Ceylan E, et al. Adiponectin as a biomarker of systemic inflammatory response in smoker patients with stable and exacerbation phases of chronic obstructive pulmonary disease. Scand.J Clin Lab Invest, 2009, 69: 219 -224.
5. 许三林, 吴人亮, 陈春莲, 等. 上皮钙黏附素在吸烟小鼠呼吸道上皮损伤修复中表达的研究. 中华结核和呼吸杂志,1999, 22:417-419.
6. Chen H, Hansen MJ, Jones JE, et al. Long-term cigarette smoke exposure increases uncoupling protein expression but reduces energy intake. Brain Res, 2008, 1228: 81-88.
7. Hotta Y, Yatsuya H, Toyoshima H, et al. Low leptin but high insulin resistance of smokers in Japanese men. Diabetes Res Clin Pract,2008, 81: 358-364 .
8. Sull JW, Kim HJ, Yun JE, et al. Serumadiponectin is associated with smoking status in healthy Korean men. Endocr J, 2009, 56: 73-78.
9. Kirchgessner TG, Uysal KT, Wiesbrock SM, et al. Tumor necrosis factor-alpha contributes to obesity-related hyperleptinemia by regulating leptin release from adipocytes. J Clin Invest, 1997, 100 :2777-2782.
10. Trujillo ME, Sullivan S, Harten I, et al. Interleukin-6 regulates human adipose tissue lipid metabolism and leptin production in vitro. J Clin Endocrinol Metab, 2004, 89 : 5577 -5582 .
11. Fantuzzi G. Adipose tissue, adipokines, and inflammation. J Allergy Clin Immunol, 2005, 115: 911 -919.
12. Gettys TW, Harkness PJ, Waston PM. The β3-aderenergic receptor inhibits insulin-stinulated leptin secretion from isolated rat adipocytes. Endocrinology, 1996, 137: 4054-4057.
13. Bruun JM, Lihn AS, Verdich C, et al. Regulation of adiponectin by adipose tissue-derived cytokines: in vivo and in vitro investigations in humans. Am J Physiol Endocrinol Metab, 2003, 285 : E527-533.
14. Iwashima Y, Katauya T, Ishikawa K, et al. Association of hypoadiponectinemia with smoking habit in men. Hypertension,2005, 45: 1094-1100 .
15. Pepys MB, Hirschfield GM. C-reactive protein: a critical update. J Clin Invest, 2003 , 111: 1805-1812.

Chinese Journal of Respiratory and Critical Care Medicine

Effects of Smoking on SerumLeptin,Adiponectin, IL-6, and C-Reactive Protein in Rats

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