SHEN Yuying , XU Xiaoyong , ZHANG Feng , ZHANG Pengpeng , SHI Yi
  • Department of Respiratory Medicine, Second Military Medical University, Nanjing General Hospital of Nanjing Military Command, PLA. Nanjing, Jiangsu,210002, ChinaCorresponding Author: SHI Yi, E-mail: shiyi56@ 126. com;
Aspergillus fumigatus; Hypha; Endothelial cell; von Willebrand factor
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Objective  To observe the levels of von Willebrand factor ( vWF) expressed by human umbilical vein endothelial cells ( HUVECs) infected by aspergillus fumigatus ( AF) alone or treatment with cytochalasin D, N-cadherin monoclonal antibody, dexamethasone, respectively, so as to explore the mechanism of angioinvasion in invasive aspergillosis. Methods An in vitro model of HUVECs infected by AF hypha was established. The experiment included six groups, ie. a sham control group, a TNF-αgroup, an AF hypha group, a cytochalasin D group, a N-cadherin antibody group, and a dexamethasone group. Cell supernatants were collected to detect the levels of vWF at 2 h, 6 h, 12 h, and 18 h by enzyme linked immunosorbent assay ( ELISA) . Results  Compared with that of vWF at 2 h, the level was higher at 18 h in the sham control
group and the TNF-αgroup, and higher at 6 h, 12 h, and 18 h in the other groups( P  lt; 0. 05) . Compared with the sham control group, the level of vWF in each experiment group increased at 2 h, 6 h, 12 h, and 18 h except that in the N-cadherin antibody group at 2 h ( P  lt; 0. 05) . The level of vWF in TNF-α group was higher than that in the AF hypha group at 2 h, but lower at 18 h. ( P  lt; 0. 05) . The level of vWF was not significantly different between the cytochalasin D group and the AF hypha group at each time point. The level of vWF was lower in the N-cadherin antibody group than that in the AF hypha group at 2 h and 6 h ( P  lt;0. 05) . The level of vWF was not significantly different between the dexamethasone group and the AF hypha group at each time point. Conclusion  HUVECs infected by AF hypha overexpress vWF. N-cadherin
monoclonal antibody can reduce the expression of vWF, but cytochalasin D or dexamethasone has no significant effect on it.

Citation： SHEN Yuying,XU Xiaoyong,ZHANG Feng,ZHANG Pengpeng,SHI Yi. Expression of von Willebrand Factor in Human Umbilical Vein Endothelial Cells Infected by Aspergillus Fumigatus Hypha. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(5): 498-500. doi: Copy