RSV-Stimulated Rat Airway Epithelial Cells Activate Myeloid Dendritic Cells_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

QIAO Jianou ¹ , JIN Xianqiao ²

Department of Respiratory Medicine, Shanghai Jiaotong University, Affiliated Ninth People’s Hospital. Shanghai, 200010, ChinaCorresponding Author: JIN Xian-qiao, E-mail: jinxianqiao@ hotmail. com;

Corresponding author：

Keywords：

Respiratory syncytial virus; Airway epithelial cells; Dendritic cells; ; Asthma; T helper type 2

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Objective Respiratory syncytial virus ( RSV) is a primary cause of lower respiratory tract infections in children, and is also the cause for the development of asthma primarily in infants. However,the immunological mechanisms by which RSV enhances allergic sensitization and asthma remain unclear. The aimof this study was to examine the influence of RSV-infected airway epithelial cells on the activation and functions of rat myeloid dendritic cells ( mDCs) . Methods Rat airway epithelial cells ( RAECs) were infected by RSV. Then RSV-infected RAECs were co-cultured with rat mDCs, and the expression of cytokine and maturation markers on mDCs were examined by real time PCR and flow cytometry. To confirm this functional mDC maturation, allergenic mixed lymphocyte reaction ( MLR) were performed. Results We
found that functional maturation of mDCs was induced by RSV-treated RAECs, as shown by their enhanced levels of OX40L and thymus- and activation-regulated chemokine ( TARC) mRNAs, which increased the expressions of major histocompatibility complex II ( MHCII) and CD86 costimulatorymolecules and promoted
T-cell proliferation in mixed lymphocyte reactions. Conclusion Our results suggest that RSV-infected epithelial cells promote the maturation of mDCs that might support Th2 cell polarization and contribute to the pathogenesis of asthma.

Citation： QIAO Jianou,JIN Xianqiao. RSV-Stimulated Rat Airway Epithelial Cells Activate Myeloid Dendritic Cells. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(6): 602-605. doi: Copy

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8.	Keane-Myers AM, Gause WC, Finkelman FD, et al. Development of murine allergic asthma is dependent upon B7-2 costimulation. J Immunol, 1998, 160: 1036-1043 .
9.	Lordan JL, Davies DE, Wilson SJ, et al. The role of CD28-B7 costimulation in allergen-induced cytokine release by bronchial mucosa from patients with moderately severe asthma. J Allergy Clin Immunol, 2001, 108: 976-981 .
10.	Tsuyuki S, Tsuyuki J, Einsle K, et al. Costimulation through B7-2( CD86) is required for the induction of a lung mucosal T helper cell 2 ( TH2) immune response and altered airway responsiveness. J Exp Med, 1997, 185 : 1671 -1679 .
11.	Imai T, Yoshida T, Baba M, et al. Molecular cloning of a novel T celldirected CC chemokine expressed in thymus by signal sequence trap using Epstein-Barr virus vector. J Biol Chem,1996,271:21514-21521.
12.	Bonecchi R, Bianchi G, Bordignon PP, et al. Differential express, on of chemokine receptors and chamotactic responsiveness of type1 Thelper cells( Thls) and Th2s. J Exp Med, 1998 , 187: 129-134 .
13.	Leung TF, Wong CK. Chan IH, et al. Plasma cocentration of thymus and activatlon-regulated chemokine is elevated in childhood asthma.Jallergy Clin lmmunol, 2002, 110: 404 -409.

1. 李明才, 何韶衡. 病毒感染在诱发和加重哮喘中的作用. 中国呼吸与危重监护杂志, 2003, 2: 189-192.
2. Becker S, Reed W, Henderson FW, et al. RSV infection of human airway epithelial cells causes production of the beta-chemokine RANTES. Am J Physiol, 1997 , 272: L512-L520 .
3. Zhang Y, Luxon BA, Casola A, et al. Expression of respiratory syncytial virus-induced chemokine gene networks in lower airway epithelial cells revealed by cDNA microarrays. J Virol,2001, 75:9044-9058.
4. 毛光宇, 杨炯, 陈宏斌, 等. 过敏性哮喘患者树突状细胞对原始T细胞活化的影响. 中国呼吸与危重监护杂志, 2005,4: 115-118.
5. Mohapatra SS, Boyapalle S. Epidemiologic, experimental, and clinical links between respiratory syncytial virus infection and asthma. Clin Microbiol Rev, 2008, 21: 495-504.
6. Lambrecht BN, De Veerman M, Coyle AJ, et al. Myeloid dendritic cells induce Th2 responsesto inhaled antigen, leading to eosinophilic airway inflammation. J Clin Invest, 2000 , 106: 551-559.
7. McAdam AJ, Schweitzer AN, Sharpe AH. The role of B7 costimulation in activation and differentiation of CD41 and CD81 T cells. Immunol Rev, 1998 , 165: 231-247 .
8. Keane-Myers AM, Gause WC, Finkelman FD, et al. Development of murine allergic asthma is dependent upon B7-2 costimulation. J Immunol, 1998, 160: 1036-1043 .
9. Lordan JL, Davies DE, Wilson SJ, et al. The role of CD28-B7 costimulation in allergen-induced cytokine release by bronchial mucosa from patients with moderately severe asthma. J Allergy Clin Immunol, 2001, 108: 976-981 .
10. Tsuyuki S, Tsuyuki J, Einsle K, et al. Costimulation through B7-2( CD86) is required for the induction of a lung mucosal T helper cell 2 ( TH2) immune response and altered airway responsiveness. J Exp Med, 1997, 185 : 1671 -1679 .
11. Imai T, Yoshida T, Baba M, et al. Molecular cloning of a novel T celldirected CC chemokine expressed in thymus by signal sequence trap using Epstein-Barr virus vector. J Biol Chem,1996,271:21514-21521.
12. Bonecchi R, Bianchi G, Bordignon PP, et al. Differential express, on of chemokine receptors and chamotactic responsiveness of type1 Thelper cells( Thls) and Th2s. J Exp Med, 1998 , 187: 129-134 .
13. Leung TF, Wong CK. Chan IH, et al. Plasma cocentration of thymus and activatlon-regulated chemokine is elevated in childhood asthma.Jallergy Clin lmmunol, 2002, 110: 404 -409.

Chinese Journal of Respiratory and Critical Care Medicine

RSV-Stimulated Rat Airway Epithelial Cells Activate Myeloid Dendritic Cells

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