Objective To investigate whether ADAM33 ( A disintegrin and metalloproteinase 33) gene polymorphismhas effect on the airway inflammation of COPD.
Methods A total of 312 COPD patients were recruited for this study. Four polymorphic loci ( T2, T1, S2, and Q-1) of ADAM33 were selected for genotyping by using the polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) method. Total and differential cell counts, contents of TNF-α, IL-6, IL-8, and VEGF in induced sputumwere detected. The relationship between genotypes and inflammatory reaction was analyzed.
Results On locus T2, the cell counts and content of TNF-αin induced sputum increased significantly in the carriers with GG genotype than those with AA and AG genotypes ( P lt;0.01 and P lt;0.05) . On locus T1, the lymphocyte counts in induced sputumincreased significantly in the carriers with GG genotype than those with AA and AG genotypes ( P lt;0.05) ; but the content of IL-8 in induced sputumwas higher in AA and AG genotypes ( P lt;0.05) . On locus Q-1, the contents of VEGF and IL-8 in induced sputum increased significantly in the carriers with GG genotype than those with AA and AG genotypes (P lt;0.05) . On locus S2, the total cell counts in induced sputumincreased significantly in the carriers with GG genotype than those with CC and CG genotypes ( P lt;0.05) , and the content of IL-8 in induced sputum increased significantly in GG genotype ( P lt;0.01 ) .
Conclusion These results suggest that ADAM33 polymorphism may participate the pathogenesis of COPD by promoting airway inflammation.
Citation： ZHANG Weibing,WANG Xinyan,TIANXiaoyan,ZHANG Hui,WANGZhipeng,SHENYan,GAO Yuyan. ADAM33 Gene Polymorphism Is Correlated with Airway Inflammation of COPD. Chinese Journal of Respiratory and Critical Care Medicine, 2012, 11(1): 15-18. doi: Copy