Left Ventricular Reconstruction in a Rat Model with Post-infarction Ventricular Aneurysm_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

KANG Kai , QU Hui , TANG Jiquan

1. Department of Cardiovascular Surgery, 2nd Affiliated Hospital of Harbin Medical University, Key Laboratory of Myocardial Ischemia Mechanism and Treatment, Ministry of Education, Harbin 150086, P. R. China;;
2. Department of Pediatrics, 2nd Affiliated Hospital of Harbin Medical University, Harbin 150086, P. R. China;;
3. Department of Cardio-thoracic Surgery, Jixi People’s Hospital, Jixi Heilongjiang 158100, P. R. China;;

Corresponding author：

Keywords：

Ventricular aneurysm; Rat; Left ventricular reconstruction

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Objective To investigate the experience of left ventricular reconstruction（LVR）in a rat model with post-infarction ventricular aneurysm. Methods A total of 35 male Sprague-Dawley （SD）rats underwent left anterior descending artery （LAD） ligation to create a left ventricular aneurysm （LVA) model following myocardial infarction. Four weeks later, 16 rats with LVA that met the inclusion criteria underwent LVR as the experiment group（LVR group）. Another 10 rats with LVA underwent thoracotomy as the control group. Three days, 2 weeks, and 4 weeks after the second operation, all the rats were examined by echocardiography to evaluate the cardiac function. At the end of the study, photography and Masson’s Trichrome staining were used to evaluate the completeness of LVA resection. Results The surgical mortality of LVA and LVR generation was 11.4%（4/35）and 18.8%（3/16）respectively， with the success rate 74.3% （26/35）for LVA model and 81.3%（13/16）for LVR model. Photography and Masson’s Trichrome staining identified complete replacement of ventricular scar by patch. Three days after the second operation, echocardiography illustrated that the left ventricular end-systolic diameter （LVESD）and fractional shortening （FS） of the LVR group were significantly improved compared with the control group （LVESD 5.00±0.87 mm versus 5.90±0.92 mm, P＜0.05，FS 34.20%± 6.80% versus 26.60%±6.12%, P＜ 0.01）. The cardiac structure and function of LVR group were also significantly improved 2 weeks and 4 weeks after the second operation compared with the control group（2 weeks：left ventricular end-diastolic diameter （LVEDD）7.60±0.56 mm versus 8.50±1.08 mm，P＜ 0.01；LVESD 5.10±0.65 mm versus 6.69±0.89 mm，P＜0.001；FS 31.90%±6.90% versus 21.10%±6.17%，P＜0.001；4 weeks：LVEDD7.70±0.50 mm versus 9.10±0.89 mm，P＜0.001；LVESD5.20±0.39 mm versus 7.20±0.95 mm，P＜0.001；FS 31.80%±2.40% versus 20.20%±4.17%，P＜0.001）. Conclusions LVR rat can be used as a stable, reliable and economic screeningmodel in engineered heart tissue（EHT）research.

Citation： KANG Kai ,QU Hui,TANG Jiquan,et al.. Left Ventricular Reconstruction in a Rat Model with Post-infarction Ventricular Aneurysm. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2012, 19(3): 293-297. doi: Copy

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3.	Zhang C, Hou J, Zheng S, et a1. Vascularized atrial tissue patch cardiomyoplasty with omentopexy improves cardiac performance after myocardial infarction. Ann Thorac Surg, 2011, 92 （4）：1435-1442.
4.	Yuan Ye K, Sullivan KE, Black LD. Encapsulation of cardiomyocytes in a fibrin hydrogel for cardiac tissue engineering. J Vis Exp, 2011, （55）：3251.
5.	Wang B, Borazjani A, Tahai M, et a1. Fabrication of cardiac patch with decellularized porcine myocardial scaffold and bone marrow mononuclear cells. J Biomed Mater Res A, 2010, 94 （4）：1100-1110.
6.	Chiu LY, Radisic M. Scaffolds with covalently immobilized VEGF and Angiopoietin-1 for vascularization of engineered tissues. Biomaterials, 2010, 31 （2）：226-241.
7.	Cui J, Li J, Mathison M, et a1. A clinically relevant large-animal model for evaluation of tissue-engineered cardiac surgical patch materials. Cardiovasc Revasc Med, 2005, 6 （3）：113-120.
8.	Ratcliffe MB, Wallace AW, Salahieh A, et a1. Ventricular volume, chamber stiffness, and function after anteroapical aneurysm plication in the sheep. J Thorac Cardiovasc Surg, 2000, 119 （1）：115-124.
9.	Pozzobon M, Bollini S, Iop L, et a1. Human bone marrow-derived CD133 （＋） cells delivered to a collagen patch on cryoinjured rat heart promote angiogenesis and arteriogenesis. Cell Transplant, 2010, 19 （10）：1247-1260.
10.	Gaebel R, Ma N, Liu J, et a1. Patterning human stem cells and endothelial cells with laser printing for cardiac regeneration. Biomaterials, 2011, 32 （35）：9218-9230.
11.	Yoshikawa E, Marui A, Tsukashita M, et a1. Carvedilol may alleviate late cardiac remodelling following surgical ventricular restoration. Eur J Cardiothorac Surg, 2010, 37 （2）：362-367.
12.	Hsu CP, Huang CY, Wang JS, et a1. Extracellular matrix remodeling attenuated after experimental postinfarct left ventricular aneurysm repair. Ann Thorac Surg, 2008, 86 （4）：1243-1249.
13.	Matsubayashi K, Fedak PW, Mickle DA, et a1. Improved left ventricular aneurysm repair with bioengineered vascular smooth muscle grafts. Circulation, 2003, 108 （Suppl 1）：II219-II225.
14.	Di Donato M, Castelvecchio S, Menicanti L. Surgical treatment of ischemic heart failure：the Dor procedure. Circ J, 2009, 73 （Suppl A）：A1-A5.
15.	Sakamoto Y, Mizuno A, Buckberg GD, et a1. Restoring the remodeled enlarged left ventricle：experimental benefits of in vivo porcine cardioreduction in the beating open heart. J Card Surg, 1998, 13 （6）：429-439.

1. 　Di Donato M, Sabatier M, Dor V, et a1. Effects of the Dor procedure on left ventricular dimension and shape and geometric correlates of mitral regurgitation one year after surgery. J Thorac Cardiovasc Surg, 2001, 121 （1）：91-96.
2. 　Horii T, Tambara K, Nishimura K, et a1. Residual fibrosis affects a long-term result of left ventricular volume reduction surgery for dilated cardiomyopathy in a rat experimental study. Eur J Cardiothorac Surg, 2004, 26 （6）：1174-1179.
3. 　Zhang C, Hou J, Zheng S, et a1. Vascularized atrial tissue patch cardiomyoplasty with omentopexy improves cardiac performance after myocardial infarction. Ann Thorac Surg, 2011, 92 （4）：1435-1442.
4. 　Yuan Ye K, Sullivan KE, Black LD. Encapsulation of cardiomyocytes in a fibrin hydrogel for cardiac tissue engineering. J Vis Exp, 2011, （55）：3251.
5. 　Wang B, Borazjani A, Tahai M, et a1. Fabrication of cardiac patch with decellularized porcine myocardial scaffold and bone marrow mononuclear cells. J Biomed Mater Res A, 2010, 94 （4）：1100-1110.
6. 　Chiu LY, Radisic M. Scaffolds with covalently immobilized VEGF and Angiopoietin-1 for vascularization of engineered tissues. Biomaterials, 2010, 31 （2）：226-241.
7. 　Cui J, Li J, Mathison M, et a1. A clinically relevant large-animal model for evaluation of tissue-engineered cardiac surgical patch materials. Cardiovasc Revasc Med, 2005, 6 （3）：113-120.
8. 　Ratcliffe MB, Wallace AW, Salahieh A, et a1. Ventricular volume, chamber stiffness, and function after anteroapical aneurysm plication in the sheep. J Thorac Cardiovasc Surg, 2000, 119 （1）：115-124.
9. 　Pozzobon M, Bollini S, Iop L, et a1. Human bone marrow-derived CD133 （＋） cells delivered to a collagen patch on cryoinjured rat heart promote angiogenesis and arteriogenesis. Cell Transplant, 2010, 19 （10）：1247-1260.
10. 　Gaebel R, Ma N, Liu J, et a1. Patterning human stem cells and endothelial cells with laser printing for cardiac regeneration. Biomaterials, 2011, 32 （35）：9218-9230.
11. 　Yoshikawa E, Marui A, Tsukashita M, et a1. Carvedilol may alleviate late cardiac remodelling following surgical ventricular restoration. Eur J Cardiothorac Surg, 2010, 37 （2）：362-367.
12. 　Hsu CP, Huang CY, Wang JS, et a1. Extracellular matrix remodeling attenuated after experimental postinfarct left ventricular aneurysm repair. Ann Thorac Surg, 2008, 86 （4）：1243-1249.
13. 　Matsubayashi K, Fedak PW, Mickle DA, et a1. Improved left ventricular aneurysm repair with bioengineered vascular smooth muscle grafts. Circulation, 2003, 108 （Suppl 1）：II219-II225.
14. 　Di Donato M, Castelvecchio S, Menicanti L. Surgical treatment of ischemic heart failure：the Dor procedure. Circ J, 2009, 73 （Suppl A）：A1-A5.
15. 　Sakamoto Y, Mizuno A, Buckberg GD, et a1. Restoring the remodeled enlarged left ventricle：experimental benefits of in vivo porcine cardioreduction in the beating open heart. J Card Surg, 1998, 13 （6）：429-439.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Left Ventricular Reconstruction in a Rat Model with Post-infarction Ventricular Aneurysm

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