YE Lianhua , XU Geng , HUANG Yunchao , et al .
  • 1.Department of Thoracic Surgery, Third Affiliated Hospital, Kunming Medical College, Kunming 650118, P. R. China;;
  • 2. Department of Thoracic and Cardiovascular Surgery, Heze Municipal Hospital, Heze 274031, Shandong, P.R. China;;
  • 3. Department of Clinical Laboratory Bacteria Room, Third Affiliated Hospital, Kunming Medical College, Kunming 650118, P.R.China;
Brominated furanone; Polyvinyl chloride; Bacterial biofilm; Staphylococcus epidermidis
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Objective To study the influence of brominated furanones on the biofilm (BF) formation of Staphylococcus epidermidis (SE) on polyvinyl chloride(PVC) materials, and provide new ideas for the research of surface modification of materials and clinical treatment of biomaterial centered infection. Methods We chose three kinds of brominated furanone with representative chemical structure for our research which were respectively 3,4dibromo-5-hydroxy2 (5H) -furanone (Mucobromic acid) in the first furanone group, 4-bromo-5(4-methoxyphenyl)3(methylamino)2(5H)furanone in the second furanone group, and 3,4dibromo-5,5-bis(4-methylphenyl)2(5H)-furanone in the third furanone group. The PVC material soaked with 75% ethanol for 5minutes was classified as the control group. The surface coating of the PVC materials in the four groups all underwent modification respectively and then they were cocultivated with staphylococcus epidermidis together. Confocal laser scanning microscope(CLSM) was adopted to detect the thickness of bacterium BF and bacterium community quantity unit area on PVC materials and scanning electron microscope(SEM) was used to observe surface structure of SE, BF formation at 6 h, 12 h, 18 h and 24 h respectively. Results The results of CLSM showed that, compared with the control group, SE bacterium community quantity unit area and the thickness of bacterium BF on the PVC material surface in the second furanone group were obviously smaller (P lt;0.05). SE bacterium community quantity unit area and thickness of bacterium BF on PVC material surface in the first and the third furanone groups had no significant difference (P gt;0.05). The result of SEM showed that, the quantity of SE bacterium community unit area on PVC material surface in the second furanone group were smaller than that of the control group at 6 hours. The biofilm structure on PVC material surface in the control group was formed at 18 hours, but there were no mature biofilm structure on PVC material surface in the second furanone group at 18 hours. Conclusion The impact of different brominated furanone on SE biofilm formation on the surface of PVC materials is different. The second kind of furanone can inhibit the quantity of SE bacterium community unit area and SE biofilm formation on the surface of PVC materials.

Citation： YE Lianhua,XU Geng,HUANG Yunchao,et al .. The Effect of Brominated Furanones on the Biofilm Formation of Staphylococcus Epidermidis on the Polyvinyl Chloride Material. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2010, 17(4): 312-316. doi: Copy