SUN Zhongdong , GAO Shangzhi , MAO Zhifu , et al .
  • 1.Department of Cardiovascular Surgery, Qingdao Municipal Hospital,Medical College of Qingdao University,Qingdao 266071, Shandong, P.R.China;;
  • 2.Department of Thoracic and Cardiovascular Surgery, Renmin Hospital, Wuhan University, Wuhan 430060,P.R.China;
Mitochondrial adenosine triphosphatesensitive potassium channel; Rabbit; Immature myocardium; Myocardial preservation
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Objective To investigate the role of mitochondrial adenosine triphosphatesensitive potassium channel(mitoKATP) in immature myocardial ischemic preconditioning, and to provide evidence for immature myocardial protection. Methods Langendorff isolated heart infused model was used in the experiment. Twentyfour rabbits (aged from 14 to 21 days) were randomly divided into 4 groups：ischemiareperfusion group（I/R group）, myocardial ischemic preconditioning group(E1 group), 5hydroxydecanoate(5-HD) group (E2 group) and Diazoxide (Diaz) group(E3 group). Hemodynamics recovery rate, myocardial water content(MWC), the leakage rates of serum creatine kinase and lactate dehydrogenase, adenosine triphosphate content, superoxide dismutase activity, malondialdehyde content, myocardial cell Ca2+ content and myocardial mitochondrial Ca2+ content, myocardial mitochondrial Ca2+-ATPase activity, the adenosine triphosphate(ATP) synthesizing ability of myocardial mitochondria were tested, and myocardial ultrastructure was observed via electron microscopy. Results The hemodynamics recovery rate, myocardial water content(P＜0.05), adenosine triphosphate content, superoxide dismutase activity, myocardial mitochondrial Ca2+-adenosine triphosphyatase(ATPase) activity and the ATP synthesizing ability of myocardial mitochondria of the rabbits in E1 and E3 group were significantly better than that in I/R group and E2 group(P＜0.05). Malondialdehyde content, the leakage rates of serum creatine kinase and lactate dehydrogenase, myocardial cell Ca2+ content and myocardial mitochondrial Ca2+ content of the rabbits in E1 group and E3 group were significantly lower than that in I/R group and E2 group (P＜0.05). The myocardial ultrastructure injury in E1 and E3 group were significantly reduced compared with that in I/R and E2 group. Conclusion Myocardial ischemic preconditioning has significant protective effects on immature myocardium. Its mechanism may be related to the activation of mitoKATP.

Citation： SUN Zhongdong,GAO Shangzhi,MAO Zhifu,et al .. The Role of Mitochondrial Adenosine Triphosphatesensitive Potassium Channel in Immature Myocardial Ischemic Preconditioning. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2009, 16(3): 210-213. doi: Copy