ZHOU Qi , ZHOU Jianye , HU Shengshou , et al .
  • 1.Key Laboratory of Health Ministry on Cardiovascular Regenerative Medicine, Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Bejing 100037, P.R. China;;
  • 2.Laboratory of Macromolecular, Department of Chemistry, Qinghua University, Beijing 100084, P.R. China;
Mesenchymal stem cell; Great omentum; Myocardial infarction; Tissue engineering; Remodeling
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Objective Engineer heart tissue (EHT) was constructed with mesenchymal stem cells (MSCs) and poly lacticacidCOglycolic acid (PLGA), and grafted onto the surface of myocardial infarction rats. We hypothesized that great omentum wrapping would increase EHT blood supply and ameliorate EHT microenvironment which is in favor of cardiac collagen remodling and heart function. We hope that omentun wrapped EHT could provide a valuable strategy for surgically myocardial infarction therapy. Methods MSCs were isolated from SD rats.Eight weeks after SD rats were subjected to left anterior descending (LAD) ligation, 18 rats were enrolled and divided into three groups, group A(n=6): great omentum wrapped MSCsPLGA EHT implantation; group B (n=6):MSCsPLGA EHT implantation; control group (n=6): the myocardial infarction; the sham group (n=6): only opened and closed chest, underwent LAD ligation, but no EHT implantation. Four weeks after transplantation, the following variables were evaluated: specimen stained with picrosirius red, left ventricle function evaluated by echocardiography, infarction ventricular wall motion by color kinesis (CK). Results Hearts of group A showed significantly less fibrosis than group B and control group (P lt;0.05). Infarction ventricular wall motion assessed by CK indicated significantly improvement in group A compared with group B and control group (P lt;0.05). Four weeks after transplantation, cardiac echocardiography showed left ventricle ejection fraction was lower in control group and group B compared with group A (P lt;0.05). Conclusion Transplantation of MSCsPLGA EHT with great omentum wrapping ameliorated infarction ventricular collagen remodeling, ameliorated infarction ventricular wall motion and preserved left ventricular function.

Citation： ZHOU Qi,ZHOU Jianye,HU Shengshou,et al .. Great Omentum Wrapped Engineer Heart Tissue Transplantation Preserves Cardiac Function in Myocardial Infarction Rats. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2008, 15(6): 432-437. doi: Copy