• 1. Department of Cardiovascular Surgery, the 1st Affiliated Hospital, Anhui Medical University ;;
  • 2. Department of Biochemistry, Anhui Medical University;;
  • 3. Department of Pathogenic Organism, Anhui Medical University, Hefei 230022, P. R. China;
Vascular endothelial growth factor; Reverse transcriptase-polymerase chain reaction; Gene transfection; Gene expression
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Objective To investigate the transfection and expression of recombinant plasmid human vascular endothelial growth factor 165/pcDNA3. 1 （hVEGF165/pcDNA3. 1） in myocardial cells, and to build foundation for gene therapy and cell therapy of coronary artery disease （CAD）. Methods Myocardial cells were cultured in vitro and transfected by hVEGF165/pcDNA3.1 with liposome; then transient expressed protein was detected by reverse transcriptase-polymerase chain reaction （RT-PCR）, immunochemistry and Western blotting. Results A strap as hVEGF165 was obtained by RT-PCR, the protein of hVEGF165 was found in myocardial cells by immunochemistry and in supernatant by Western blotting. Conclusion The recombinant plasmid hVEGFI65/pcDNA3. 1 can be expressed in myocardial cells, and may be used in studying CAD by gene therapy and cell transplantation.

Citation： ZHOU Zhengchun,GE Jianjun,CHEN Bing,et al .. hVEGF165/pcDNA3. 1 Expression in Neonate Mice Myocardial Cells. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2006, 13(3): 180-183. doi: Copy