• 1. Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, P. R. China;;
  • 2. Department of Cardiac Surgery, Union Itospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, P. R. China;
Rat; Lung transplantation; Cuff anastomosis method
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Objective To establish a simple, valid rat orthotopic left lung transplantation model with the improved operation technique. Methods One hundred and thirty-six male SD rats were randomly divided into donor （n = 68） and recipient （n = 68）, transplantation were performed by using the improved cuff anastomosis technique. Results Time of donor lung perfusion-picking, donor lung vessel cuff anastomosis and recipient vessel anastomosis was 13±2 min, 9±1 min, 10±1 min respectively, the operative time was 60±3 min. In 68 rats of operations, successful rate was 88%（60/68）, anastomotic stoma leak in one rat, lung congestion 3 rats, lung atelectasis 4 rats. The shortest survival time was 1 day, there were 53 rats whose survival time was longer than 12 days. The chest computed tomography showed no atelectasis and blood gas analysis manifested good respiratory function. Conclusion The improved three cuff anastomosis technique offers a simple, valid, cheap and useful method,it can establish rat orthotopic left lung transplantation model successfully.

Citation： JIANG Ke,WANG Jianjun,CHEN Jun,et al .. The Improvements in Establishment of Rat Orthotopic Left Lung Transplantation Model. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2006, 13(5): 335-337. doi: Copy