GE Jianjun , ZHOU Zhengchun , CHEN Bing , et al .
  • 1. Department of cardiovascular surgery , the First Affiliated Hospital of Anhui Medical University , Hefei 230022,P.R.China;;
  • 2. Department of Biochemistry and Molecularbiology , Anhui Medical University , Hefei 230022 , P.R. Chia ;;
  • 3. Department of Pathophysiology , Anhui Medical University , Hefei 230022, P.R.China.;
Vascular endothelial growth factor; Gene clone; Gene expression
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Objective To study clone human vascular endothelial growth factor gene165(hVEGF165) to construct the recombined plasmid pcDNA 3.1/hVEGF165 and observe its expression in COS-7. Methods hVEGF gene was amplified by reverse transcription polymerase chain reaction(RT-PCR) method from fetal human myocardium tissue, was then loned into T-vector; identified by polymerase chain reaction(PCR); and was inserted into the expression plasmid pcDNA3.1 to construct the recombined plasmids that encoding VEGF165 comlementary DNA(cDNA). COS-7 cells were transfected mediated by liposome, then expressed protein was detected by Western blotting. Results Exact gene order of hVEGF165 was obtained from the fetal human myocardium tissue by RT-PCR; pcDNA3.1/VEGF165 was constructed, and transient expression was going after transfecting COS-7 cell. Conclusion The recombined plasmids we constructed could successfully express the hVEGF protein after eukaryotic cells COS-7 were transfected.

Citation： GE Jianjun,ZHOU Zhengchun,CHEN Bing,et al .. hVEGF165 Gene Clone and Its Expression in COS-7 Cells. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2005, 12(3): 185-188. doi: Copy