EXPERIMENTAL STUDY ON PROTECTIVE EFFECT OF EARLY MOTION ON ARTICULAR CARTILAGE AFTER JOINT ALLOGRAFT_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

LI Xiang , ZHANG Youle , DENG Jiuzheng

Department of Hand Surgery, Beijing Jishuitan Hospital, the Fourth Clinical School Medicine of Peking University, Beijing, 100035, P.R.China.;

Corresponding author：

Keywords：

Joint allograft ，Articular cartilage ，Early motion ，Rabbit

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【Abstract】 Objective To investigate the protective effect of early motion on articular cartilage after joint allograft by performing a controlled trial between different post-operation strategies after joint allograft in an animal model. Methods Twenty hemi-knee joints were harvested from 10 6-month-old New Zealand white rabbits (male or female, weighing 2.5-3.0 kg); 10 hemi-knee joints by deep frozen treatment (donors) were transplanted to unilateral knee joints (recipients) of 10 6-month-old Chinchilla rabbits (male or female, weighing 2.5-3.0 kg), which were divided into early motion group (n=5) and sustained fixation group (n=5); and 10 hemi-knee joints were used as blank control (n=5) and frozen control (n=5). The articular cartilage of allogenic joints was detected by X-ray film, gross, and histology at 6 weeks after operation. Results Gross observation: no obvious limitation of joint movements was observed in early motion group, but obvious limitation in sustained fixation group. X-ray films: the bone ends between donor and recipient healed well with good paraposition and alignment on the operation day and 2 weeks after operation; at 6 weeks, angulation deformity was observed in early motion group of 3 rabbits, and paraposition and alignment were satisfactory in sustained fixation group. Histological observation: HE staining showed that the chondrocytes had normal quantity and morphology with few nuclear fragmentation and karyolysis in early motion group, but the quantity of chondrocytes sharply decreased with dissolved nuclei and numerous fibrous tissues in the cartilage matrix in sustained fixation group. The cell survival rate of the early motion group (49.66% ± 2.15%) was significantly higher than that of the sustained fixation group (20.68% ± 1.24%) (P lt; 0.05). Scanning electron microscopy observation: nuclear membrane was intact with chromatin condensation and edema of mitochondria and rough surfaced endoplasmic reticulum in early motion group, and that the membrane of chondrocyte vanished with blurring border between chondrocyte and matrix, rupture of nuclear membrane and the disappearance of chromatin and organelles could be found in sustained fixation group. Conclusion Early motion has protective effect on articular cartilage after joint allograft, but cannot completely prevent degeneration of the allogenic articular cartilage.

Citation： LI Xiang,ZHANG Youle,DENG Jiuzheng. EXPERIMENTAL STUDY ON PROTECTIVE EFFECT OF EARLY MOTION ON ARTICULAR CARTILAGE AFTER JOINT ALLOGRAFT. Chinese Journal of Reparative and Reconstructive Surgery, 2012, 26(1): 26-30. doi: Copy

1.	allografts: long-term survival, but frequent complications. Clin Orthop Relat Res, 2010, 468(11): 2914-2923.
2.	in porcine articular chondrocytes. Cryobiology, 2010, 61(3): 297-302.
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4.	transplantation of human femoral iaphysis and total knee joints—first clinical experiences. Transplant Proc, 1998, 30(6): 2754-2761.
5.	joints. The first 18 years of basic research and its clinical application.
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7.	Orthop Clin North Am, 1987, 18(2): 275-289.
8.	lesions of the talar shoulder treated with fresh osteochondral allograft transplantation. J Bone Joint Surg (Am), 2011, 93(7): 648-654.
9.	defect of the lateral femoral condyle. Knee Surg Sports Traumatol.
10.	Arthrosc, 2011, 19(4): 556-558.
11.	of the acetabulum with osteochondral allograft transplantation. Orthopedics, 2011, 34(7): e307-311.
12.	Transplant Proc, 2011, 43(2): 652-654.
13.	文雪, 张朝跃. 同种异体半关节移植治疗下肢关节端骨肿瘤. 现代生物医学进展, 2010, 10(13): 2476-2478.
14.	刘跃辉, 屠重棋, 张开伟, 等. 同种异体骨关节移植并钢板置入内固定治疗桡骨远端骨巨细胞瘤9例. 中国组织工程研究与临床康复, 2009, 13(43): 8573-8577.
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16.	Ogilvie CM, Crawford EA, Hosalkar HS, et al. Long-term results for limb salvage with osteoarticular allograft reconstruction. Clin Orthop Relat Res, 2009, 467(10): 2685-2690.
17.	Song YC, An YH, Kang QK, et al. Vitreous preservation of articular cartilage grafts. J Invest Surg, 2004, 17(2): 65-70.
18.	Jomha NM, Weiss AD, Fraser Forbes J, et al. Cryoprotectant agent toxicity.
19.	Ohlendorf C, Tomford WW, Mankin HJ. Chondrocyte survival in cryopreserved osteochondral articular cartilage. J Orthop Res, 1996, 14(3): 413-416.
20.	Linn MS, Chase DC, Healey RM, et al. Etanercept enhances preservation.
21.	Hofmann GO, Kirschner MH, Wagner FD, et al. Allogeneic vascularized.
22.	Diefenbeck M, Wagner F, Kirschner MH, et al. Outcome of allogeneic vascularized knee transplants. Transpl Int, 2007, 20(5): 410-418.
23.	Diefenbeck M, Nerlich A, Schneeberger S, et al. Allograft vasculopathy after allogeneic vascularized knee transplantation. Transpl Int, 2011, 24(1): e1-5.
24.	李也白, 叶忠, 历智, 等. 采用大块同种异体骨和复合手术治疗骨缺损(附46例异体骨和半关节移植). 中华骨科杂志, 1995, 15(1): 3-9.
25.	Salter RB. The biologic concept of continuous passive motion of synovial.
26.	杨贵勇, 卢世璧. 关节软骨损伤修复基础研究进展. 中国矫形外科杂志, 1996, 3(1): 54-55.
27.	Mankin HJ, Gebhardt MC, Tomford WW. The use of frozen cadaveric allografts in the management of patients with bone tumors of the extremities.
28.	Adams SB Jr, Viens NA, Easley ME, et al. Midterm results of osteochondral.
29.	Lee JH, Wang SI, Choi HR, et al. Unicondylar osteoarticular allograft reconstruction of the distal femur in a patient with a traumatic osteoaticular.
30.	郑小飞, 黄华扬, 丁焕文, 等. 大块同种异体骨移植重建骨肿瘤巨大骨缺损78例. 中国组织工程研究与临床康复, 2009, 13(15): 2978-2983.
31.	Krych AJ, Lorich DG, Kelly BT. Treatment of focal osteochondral defects.
32.	Hossein EM, Ashraf H, Peivandi L. Total allograft transplantation of the elbow joint after wide resection of synovial cell sarcoma: a case series.

1. allografts: long-term survival, but frequent complications. Clin Orthop Relat Res, 2010, 468(11): 2914-2923.
2. in porcine articular chondrocytes. Cryobiology, 2010, 61(3): 297-302.
3. of osteochondral allograft viability. Am J Sports Med, 2011, 39(7): 1494-1499.
4. transplantation of human femoral iaphysis and total knee joints—first clinical experiences. Transplant Proc, 1998, 30(6): 2754-2761.
5. joints. The first 18 years of basic research and its clinical application.
6. Clin Orthop Relat Res, 1989, (242): 12-25.
7. Orthop Clin North Am, 1987, 18(2): 275-289.
8. lesions of the talar shoulder treated with fresh osteochondral allograft transplantation. J Bone Joint Surg (Am), 2011, 93(7): 648-654.
9. defect of the lateral femoral condyle. Knee Surg Sports Traumatol.
10. Arthrosc, 2011, 19(4): 556-558.
11. of the acetabulum with osteochondral allograft transplantation. Orthopedics, 2011, 34(7): e307-311.
12. Transplant Proc, 2011, 43(2): 652-654.
13. 文雪, 张朝跃. 同种异体半关节移植治疗下肢关节端骨肿瘤. 现代生物医学进展, 2010, 10(13): 2476-2478.
14. 刘跃辉, 屠重棋, 张开伟, 等. 同种异体骨关节移植并钢板置入内固定治疗桡骨远端骨巨细胞瘤9例. 中国组织工程研究与临床康复, 2009, 13(43): 8573-8577.
15. Toy PC, White JR, Scarborough MT, et al. Distal femoral osteoarticular.
16. Ogilvie CM, Crawford EA, Hosalkar HS, et al. Long-term results for limb salvage with osteoarticular allograft reconstruction. Clin Orthop Relat Res, 2009, 467(10): 2685-2690.
17. Song YC, An YH, Kang QK, et al. Vitreous preservation of articular cartilage grafts. J Invest Surg, 2004, 17(2): 65-70.
18. Jomha NM, Weiss AD, Fraser Forbes J, et al. Cryoprotectant agent toxicity.
19. Ohlendorf C, Tomford WW, Mankin HJ. Chondrocyte survival in cryopreserved osteochondral articular cartilage. J Orthop Res, 1996, 14(3): 413-416.
20. Linn MS, Chase DC, Healey RM, et al. Etanercept enhances preservation.
21. Hofmann GO, Kirschner MH, Wagner FD, et al. Allogeneic vascularized.
22. Diefenbeck M, Wagner F, Kirschner MH, et al. Outcome of allogeneic vascularized knee transplants. Transpl Int, 2007, 20(5): 410-418.
23. Diefenbeck M, Nerlich A, Schneeberger S, et al. Allograft vasculopathy after allogeneic vascularized knee transplantation. Transpl Int, 2011, 24(1): e1-5.
24. 李也白, 叶忠, 历智, 等. 采用大块同种异体骨和复合手术治疗骨缺损(附46例异体骨和半关节移植). 中华骨科杂志, 1995, 15(1): 3-9.
25. Salter RB. The biologic concept of continuous passive motion of synovial.
26. 杨贵勇, 卢世璧. 关节软骨损伤修复基础研究进展. 中国矫形外科杂志, 1996, 3(1): 54-55.
27. Mankin HJ, Gebhardt MC, Tomford WW. The use of frozen cadaveric allografts in the management of patients with bone tumors of the extremities.
28. Adams SB Jr, Viens NA, Easley ME, et al. Midterm results of osteochondral.
29. Lee JH, Wang SI, Choi HR, et al. Unicondylar osteoarticular allograft reconstruction of the distal femur in a patient with a traumatic osteoaticular.
30. 郑小飞, 黄华扬, 丁焕文, 等. 大块同种异体骨移植重建骨肿瘤巨大骨缺损78例. 中国组织工程研究与临床康复, 2009, 13(15): 2978-2983.
31. Krych AJ, Lorich DG, Kelly BT. Treatment of focal osteochondral defects.
32. Hossein EM, Ashraf H, Peivandi L. Total allograft transplantation of the elbow joint after wide resection of synovial cell sarcoma: a case series.

Chinese Journal of Reparative and Reconstructive Surgery

EXPERIMENTAL STUDY ON PROTECTIVE EFFECT OF EARLY MOTION ON ARTICULAR CARTILAGE AFTER JOINT ALLOGRAFT

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