• 1Department of Orthopaedics, 3Department of Operating Room, XinqiaoHospital, the Third Military Medical University, Chongqing, 400037, P.R.China;;
  • 2 Health Department of 93197 Unit of Chinese PLA;;
  • 4Department of Orthopaedics, the 187th Hospital of Chinese PLA.;
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【Abstract】 Objective To detect the expression of Bcl-2/adenovirus E1B 19-kDa-interacting protein 3 (BNIP3)
in cell death induced by nutrition deprivation in nucleus pulposus cells so as to further understand the mechanism of death
in nucleus pulposus cells. Methods Two adult Sprague Dawley rats, male or female, weighing 150-200 g, were involved
in this experiment. The cells isolated from rat caudal disc were cultured under the condition of L-DMEM culture media,
10%FBS, and 21%O2 (control group) and under the condition of DMEM-free glucose culture media, no serum, and 1% O2
(experimental group). The expressions of BNIP3 gene and protein were detected by real-time fluorescent quantitative PCR,
immunofluorescence staining, and Western blot. The cell apoptosis rate and mitochondrial membrane potential were measured
by flow cytometry at 24, 48, and 72 hours after culture. Results The expression of BNIP3 decreased in the control group;
the expressions of BNIP3 showed an increasing tendency with time in the experimental group, and BNIP3 combined with
mitochondria. Significant differences were observed in the expressions of BNIP3 gene and protein between 2 groups at the other
time (P  lt; 0.05) except that no significant difference was observed in the expression of BNIP3 gene at 24 hours (P  gt; 0.05). The
cell apoptosis rate and mitochondrial membrane potential were significantly lower in the experimental group than those in the
control group (P  lt; 0.05). Conclusion Upregulation of BNIP3 and translocation to mitochondria may be involved in nucleus
pulposus cell death in nutrition deprivation.

Citation: LIU Jie,PENG Na,ZENG Shunfu,LU Yan,WANG Jian,ZHOU Yue.. UPREGULATION OF Bcl-2/ADENOVIRUS E1B 19-kDa-INTERACTING PROTEIN 3 AND TRANSLOCATION TOMITOCHONDRIA IN NUCLEUS PULPOSUS CELLS INDUCED BY NUTRITION DEPRIVATION. Chinese Journal of Reparative and Reconstructive Surgery, 2012, 26(2): 166-171. doi: Copy