EFFECT OF EARLY DERMATOPLASTIC REPAIR ON TESTICULAR Survivin PROTEIN EXPRESSION IN JUVENILE PIGS WITH ENTIRE THIRD DEGREE BURN WOUND OF SCROTUM_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

TANG Xiujun , WEI Zairong , SUN Guangfeng , DENG Chengliang , WANG Dali.

Department of Burns and Plastic Surgery, the Affiliated Hospital of Zunyi Medical College, Zunyi Guizhou, 563000, P.R.China.;

Corresponding author：

Keywords：

Scrotum burn; Dermatoplasty; Spermatogenic function; Pig

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

【Abstract】 Objective To explore the effect of early scrotal dermatoplasty on spermatogenic functional rehabilitation of testis in juvenile pigs with third degree burn wound of the scrotum. Methods Thirty healthy male Guizhou miniature pigs (weighing 10-15 kg, 2-month-old) were divided into 3 groups: control group (group A, n=10), natural healing group (group B, n=10), and dermatoplasty group (group C, n=10). In group A, the pig was not given any treatment; after third degree burn model of the scrotum was prepared, wounds were not treated in group B and the burn skin was excised and whole hypogastric pachydermia was used for dermatoplasty in group C. At 3 months and 1 year after model preparation, bilateral testis were collected from 5 pigs, respectively. HE staining was performed to observe the effects of different repair method on the morphology of spermatogenic cells and immunohistochemical staining was used to detect Survivin protein expression. Results All pigs survived to the end of the experiment and the wound healed successfully. Histological observation showed that spermatogenic cells had normal shape at all stages and mature sperms were seen in lumens in group A; the thickness of seminiferous epithelium was thinner, having one layer or two layers of spermatogenic cells in group B; the spermatogenic cells in group C were slightly more than that in group B with some spermatids; and in groups B and C, the spermatogenic cells at 1 year were more than that at 3 months. Immunohistochemistry staining showed that the Survivin protein expression in groups B and C was less than in group A, and group B was less than group C, showing significant differences at 3 months and 1 year (P lt; 0.05), but no significant difference between 3 months and 1 year in the same group (P gt; 0.05). Conclusion Dermatoplasty has inhibitory effect on spermatogenic functional rehabilitation of testis. Dermatoplasty can decrease spermatogenic cells and reduce Survivin protein expression, but some spermatids still survive in seminiferous tubule.

Citation： TANG Xiujun,WEI Zairong,SUN Guangfeng,DENG Chengliang,WANG Dali.. EFFECT OF EARLY DERMATOPLASTIC REPAIR ON TESTICULAR Survivin PROTEIN EXPRESSION IN JUVENILE PIGS WITH ENTIRE THIRD DEGREE BURN WOUND OF SCROTUM. Chinese Journal of Reparative and Reconstructive Surgery, 2012, 26(3): 326-329. doi: Copy

1.	蒋波, 覃宝笋, 徐贻良, 等. 阴囊皮肤化学性烧伤4例报告. 临床泌尿外科杂志, 2007, 22(1): 70-71.
2.	康群军, 余群洪, 胡葵葵, 等. 下腹部岛状皮瓣在阴囊毁损性电烧伤修复中的应用. 组织工程与重建外科杂志, 2010, 6(1): 28-30.
3.	Wang DL, Zheng H, Deng F. Spermatogenesis after scrotal reconstruction. Br J Plast Surg, 2003, 56(5): 484-488.
4.	孙广峰, 王达利, 李丽, 等. 皮瓣重建兔阴囊诱导精原细胞凋亡与热休克蛋白70表达. 中华男科学杂志, 2010, 16(7): 606-610.
5.	王达利, 魏在荣, 祁建平, 等. 阴囊再造术后皮瓣修薄对睾丸生精功能的影响一例. 中华外科杂志, 2007, 45(23): 1655-1656．.
6.	Wang DL, Luo ZJ, Sun GF, et al. Long-term prognosis of free skin-grafted peno-scrotal avulsion injuries in two patients. J Plast Reconstr Aesthet Surg, 2009, 62(3): 385-387.
7.	黎鳌. 实验烧伤外科学. 重庆: 重庆大学出版社, 1997: 33-41.
8.	李和平, 贺晓营. 中国小型猪的开发利用. 郑州牧专学报, 1996, 16(2): 34-35.
9.	Horta R, Cerqueira M, Marques M, et al. Fournier’s gangrene: from urological emergency to plastic surgery. Actas Urol Esp, 2009, 33(8): 925-929.
10.	Hsu H, Lin CM, Sun TB, et al. Unilateral gracilis myofasciocutaneous Advancement flap for single stage reconstruction of scrotal and perineal defects. J Hast Reconstr Aesther Surg, 2007, 60(9): 1055-1059．.
11.	程君涛, 李小毅. 阴股沟双皮瓣阴囊再造术治愈阴囊高压电击伤一例. 中华烧伤杂志, 2006, 22(6): 476.
12.	苗平, 周明武, 葛华平. 股上部直接动脉皮瓣修复阴茎及阴囊皮肤缺损. 中国修复重建外科杂志, 2009, 23(10): 1278-1280.
13.	Karaçal N, Livaoglu M, Kutlu N, et al. Scrotum reconstruction with neuron- vascular pedicled pudendal thigh flaps. Urology, 2007, 70(1): 170-172.
14.	Ferreira PC, Reis JC, Amarante JM, et al. Fournier’s gangrene: a review of 43 reconstructive cases. Plast Reconstr Surg, 2007, 119(1): 175-184.
15.	Selvaggi G, Hoebeke P, Ceulemans P, et al. Scrotal reconstruction in female-to-male transsexuals: a novel scrotoplasty. Plast Reconstr Surg, 2009, 123(6): 1710-1718.
16.	Zhao J, Tenev T, Martins LM, et al. The ubiquitin-proteasome pathway reguIates surviving degradation in a cell cycle dependent manner. J Cell Sci, 2000, 113 Pt 23: 4363-4371.
17.	王达利, 孙广峰, 魏在荣, 等. 腹股沟区皮下埋藏睾丸对兔精子发生的影响. 中国修复重建外科杂志, 2008, 22(7): 842-845.
18.	杨艳清, 江昌艳, 秦巍, 等. 阴茎阴囊皮肤撕脱伤的治疗. 中国修复重建外科杂志, 2003, 17(4): 311.

1. 蒋波, 覃宝笋, 徐贻良, 等. 阴囊皮肤化学性烧伤4例报告. 临床泌尿外科杂志, 2007, 22(1): 70-71.
2. 康群军, 余群洪, 胡葵葵, 等. 下腹部岛状皮瓣在阴囊毁损性电烧伤修复中的应用. 组织工程与重建外科杂志, 2010, 6(1): 28-30.
3. Wang DL, Zheng H, Deng F. Spermatogenesis after scrotal reconstruction. Br J Plast Surg, 2003, 56(5): 484-488.
4. 孙广峰, 王达利, 李丽, 等. 皮瓣重建兔阴囊诱导精原细胞凋亡与热休克蛋白70表达. 中华男科学杂志, 2010, 16(7): 606-610.
5. 王达利, 魏在荣, 祁建平, 等. 阴囊再造术后皮瓣修薄对睾丸生精功能的影响一例. 中华外科杂志, 2007, 45(23): 1655-1656．.
6. Wang DL, Luo ZJ, Sun GF, et al. Long-term prognosis of free skin-grafted peno-scrotal avulsion injuries in two patients. J Plast Reconstr Aesthet Surg, 2009, 62(3): 385-387.
7. 黎鳌. 实验烧伤外科学. 重庆: 重庆大学出版社, 1997: 33-41.
8. 李和平, 贺晓营. 中国小型猪的开发利用. 郑州牧专学报, 1996, 16(2): 34-35.
9. Horta R, Cerqueira M, Marques M, et al. Fournier’s gangrene: from urological emergency to plastic surgery. Actas Urol Esp, 2009, 33(8): 925-929.
10. Hsu H, Lin CM, Sun TB, et al. Unilateral gracilis myofasciocutaneous Advancement flap for single stage reconstruction of scrotal and perineal defects. J Hast Reconstr Aesther Surg, 2007, 60(9): 1055-1059．.
11. 程君涛, 李小毅. 阴股沟双皮瓣阴囊再造术治愈阴囊高压电击伤一例. 中华烧伤杂志, 2006, 22(6): 476.
12. 苗平, 周明武, 葛华平. 股上部直接动脉皮瓣修复阴茎及阴囊皮肤缺损. 中国修复重建外科杂志, 2009, 23(10): 1278-1280.
13. Karaçal N, Livaoglu M, Kutlu N, et al. Scrotum reconstruction with neuron- vascular pedicled pudendal thigh flaps. Urology, 2007, 70(1): 170-172.
14. Ferreira PC, Reis JC, Amarante JM, et al. Fournier’s gangrene: a review of 43 reconstructive cases. Plast Reconstr Surg, 2007, 119(1): 175-184.
15. Selvaggi G, Hoebeke P, Ceulemans P, et al. Scrotal reconstruction in female-to-male transsexuals: a novel scrotoplasty. Plast Reconstr Surg, 2009, 123(6): 1710-1718.
16. Zhao J, Tenev T, Martins LM, et al. The ubiquitin-proteasome pathway reguIates surviving degradation in a cell cycle dependent manner. J Cell Sci, 2000, 113 Pt 23: 4363-4371.
17. 王达利, 孙广峰, 魏在荣, 等. 腹股沟区皮下埋藏睾丸对兔精子发生的影响. 中国修复重建外科杂志, 2008, 22(7): 842-845.
18. 杨艳清, 江昌艳, 秦巍, 等. 阴茎阴囊皮肤撕脱伤的治疗. 中国修复重建外科杂志, 2003, 17(4): 311.

Chinese Journal of Reparative and Reconstructive Surgery

EFFECT OF EARLY DERMATOPLASTIC REPAIR ON TESTICULAR Survivin PROTEIN EXPRESSION IN JUVENILE PIGS WITH ENTIRE THIRD DEGREE BURN WOUND OF SCROTUM

Abstract Full text Figures/Tables Video References Cited by

Format

Content