TENSILE MECHANICAL CHARACTERISTICS OF DECALCIFIED CORTICAL BONE MATRIX_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

LIU Guoming ¹ , ZHANG Yi ^2,3 , JIANG Yanlin ³ , HUANG Fuguo ¹ , QIN Tingwu ^2,3

1Department of Orthopedics, 2Institute of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, 3Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P.R.China.;

Corresponding author：

Keywords：

Decalcified cortical bone matrix; Mechanical characteristics; Tissue engineered scaffold

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To evaluate the tensile mechanical characteristics of decalcified cortical bone matrix with different thicknesses so as to provide an experimental basis for the scaffold of tissue engineering. Methods Decalcified cortical bone matrix was prepared from fresh bovine tibia with rapid decalcification techniques. Its physical characteristics including colour, texture, and so on, were observed. Then the decalcified rate was calculated. Decalcified cortical bone matrices were radially cut into sl ices with different thicknesses along longitudinal axis and divided into 4 groups: group A (100- 300 μm), group B (300-500 μm), group C (500-700 μm), and group D (700-1 000 μm). Then the sl ice specimens of each group were characterized with tensile test and histological examination. Results General observation showed that decalcified cortical bone matrix with hydrogen peroxide treatment was ivory white with good elasticity and flexibil ity. The decalcified rate was 97.6%. The tensile strength and elastic modulus of groups B, C, and D were significantly higher than those of roup A (P lt; 0.05); there was no significant difference among groups B, C, and D (P gt; 0.05). The stiffness in 4 groups increased gradually with the increasing thickness, it was significantly lower in group A than those in groups B, C, and D (P lt; 0.05), and in groups B and C than that in group D (P lt; 0.05). While there was no significant difference in ultimate strain within 4 groups (P gt; 0.05). Histologically, intact osteon was observed in every group, with an average maximum diameter of 182 μm (range, 102- 325 μm). Conclusion The mechanical properties of decalcified cortical bone matrix might depend on the integrity of the osteons. Sl ices with thickness of 300 μm or more could maintain similar mechanical properties when decalcified cortical bone matrix is used as a scaffold for tissue engineering.

Citation： LIU Guoming,ZHANG Yi,JIANG Yanlin,HUANG Fuguo,QIN Tingwu,.. TENSILE MECHANICAL CHARACTERISTICS OF DECALCIFIED CORTICAL BONE MATRIX. Chinese Journal of Reparative and Reconstructive Surgery, 2012, 26(4): 501-505. doi: Copy

1.	Rho JY, Kuhn-Spearing L, Zioupos P. Mechanical properties and the hierarchical structure of bone. Med Eng Phys, 1998, 20(2): 92-102.
2.	Swadener JG, Rho JY, Pharr GM. Effects of anisotropy on elastic moduli measured by nanoindentation in human tibial cortical bone. J Biomed Mater Res, 2001, 57(1): 108-112.
3.	Novitskaya E, Chen PY, Lee S, et al. Anisotropy in the compressive mechanical properties of bovine cortical bone and the mineral and protein constituents. Acta Biomater, 2011, 7(8): 3170-3177.
4.	Martin RB, Ishida J. The relative effects of collagen fiber orientation, porosity, density, and mineralization on bone strength. J Biomech, 1989, 22(5): 419-426.
5.	Urist MR, Dawson E. Intertransverse process fusion with the aid of chemosterilized autolyzed antigen-extracted allogeneic (AAA) bone. Clin Orthop Relat Res, 1981, (154): 97-113.
6.	Urist MR, Silverman BF, Büring K, et al. The bone induction principle. Clin Orthop Relat Res, 1967, (53): 243-283.
7.	Bigham AS, Dehghani SN, Shafiei Z, et al. Xenogenic demineralized bone matrix and fresh autogenous cortical bone effects on experimental bone healing: radiological, histopathological and biomechanical evaluation. J Orthop Traumatol, 2008, 9(2): 73-80.
8.	Mistry AS, Mikos AG. Tissue engineering strategies for bone regeneration. Adv Biochem Eng Biotechnol, 2005, 94: 1-22.
9.	Sasso RC, LeHuec JC, Shaffrey C, et al. Iliac crest bone graft donor site pain after anterior lumbar interbody fusion: a prospective patient satisfaction outcome assessment. J Spinal Disord Tech, 2005, 18(Suppl): S77-81.
10.	Silber JS, Anderson DG, Daffner SD, et al. Donor site morbidity after anterior iliac crest bone harvest for single-level anterior cervical discectomy and fusion. Spine (Phila Pa 1976), 2003, 28(2): 134-139.
11.	Buck BE, Resnick L, Shah SM, et al. Human immunodeficiency virus cultured from bone. Implications for transplantation. Clin Orthop Relat Res, 1990, (251): 249-253.
12.	Honsawek S, Bumrungpanichthaworn P, Thanakit V. Osteoinductive potential of small intestinal submucosa/demineralized bone matrix as composite scaffolds for bone tissue engineering. Asian Biomed, 2010, 4 (6): 913-922.
13.	Guizzardi S, Di Silvestre M, Scandroglio R, et al. Implants of heterologous demineralized bone matrix for induction of posterior spinal fusion in rats. Spine (Phlia Pa 1976), 1992, 17(6): 701-707.
14.	Pietrzak WS, Perns SV, Keyes J, et al. Demineralized bone matrix graft: a scientific and clinical case study assessment. J Foot Ankle Surg, 2005, 44(5): 345-353.
15.	Honsawek S, Dhitiseith D, Phupong V. Gene expression characteristics of osteoblast differentiation in human umbilical cord mesenchymal stem cells induced by demineralized bone matrix. Asian Biomed, 2007, 1(4): 383-391.
16.	Kasten P, Luginbühl R, van Griensven M, et al. Comparison of human bone marrow stromal cells seeded on calcium-deficient hydroxyapatite, beta-tricalcium phosphate and demineralized bone matrix. Biomaterials, 2003, 24(15): 2593-603.
17.	Xie H, Yang F, Deng L, et al. The performance of a bone-derived scaffold material in the repair of critical bone defects in a rhesus monkey model. Biomaterials, 2007, 28(22): 3314-3324.
18.	Honsawek S, Bumrungpanichthaworn P, Thitiset T, et al. Gene expression analysis of demineralized bone matrix-induced osteogenesis in human periosteal cells using cDNA array technology. Genet Mol Res, 2011, 10(3): 2093-2103.
19.	Jackson DW, Simon TM, Lowery W, et al. Biologic remodeling after anterior cruciate ligament reconstruction using a collagen matrix derived from demineralized bone. An experimental study in the goat model. Am J Sports Med, 1996, 24(4): 405-414.
20.	Yamada T. Bone-demineralized bone-bone graft for ligament reconstruction in rats. J Med Dent Sci, 2004, 51(1): 45-52.
21.	Burstein AH, Zika JM, Heiple KG, et al. Contribution of collagen and mineral to the elastic-plastic properties of bone. J Bone Joint Surg (Am), 1975, 57(7): 956-961.
22.	Paterson CR. Collagen chemistry and the brittle bone diseases. Endeavour, 1988, 12(2): 56-59.
23.	Wang X, Bank RA, Tekoppele JM, et al. The role of collagen in determining bone mechanical properties. J Orthop Res, 2001, 19(6): 1021-1026.
24.	侯振德, 高瑞亭, 周欣竹. 干牛骨拉伸弹性模量沿径向的分布. 中国生物医学工程学报, 1995, 14(2): 149-154.
25.	Bowman SM, Zeind J, Gibson LJ, et al. The tensile behavior of demineralized bovine cortical bone. J Biomech, 1996, 29(11): 1497-501.
26.	Catanese J 3rd, Iverson EP, Ng RK, et al. Heterogeneity of the mechanical properties of demineralized bone. J Biomech, 1999, 32(12): 1365-1369.
27.	Omae H, Zhao C, Sun YL, et al. Multilayer tendon slices seeded with bone marrow stromal cells: a novel composite for tendon engineering. J Orthop Res, 2009, 27(7): 937-942.
28.	Mauney JR, Sjostorm S, Blumberg J, et al. Mechanical stimulation promotes osteogenic differentiation of human bone marrow stromal cells on 3-D partially demineralized bone scaffolds in vitro. Calcif Tissue Int, 2004, 74(5): 458-468.
29.	向强, 邓聪颖, 张远, 等. 成骨细胞特异性钙黏蛋白涂布脱钙骨基质材料对BMSCs 黏附及成骨分化能力的影响. 中国修复重建外科杂志, 2009, 23(5): 602-606.
30.	Hou T, Li Q, Luo F, et al. Controlled dynamization to enhance reconstruction capacity of tissue-engineered bone in healing critically sized bone defects: an in vivo study in goats. Tissue Eng Part A, 2010, 16(1): 201-212.
31.	侯天勇, 罗飞, 刘杰, 等. 冻干组织工程骨与组织工程骨成骨活性比较研究. 中国修复重建外科杂志, 2010, 24(7): 779-784.
32.	杨军, 周振东, 李建军, 等. 动态压力促进骨基质支架中微血管形成的实验研究. 中华骨科杂志, 2011, 31(4): 372-378.
33.	Pan Y, Dong SW, Hao Y, et al. Demineralized bone matrix gelatin as scaffold for tissue engineering. Afr J Microbiol Res, 2010, 4(9): 865-870.
34.	Summitt MC, Reisinger KD. Characterization of the mechanical properties of demineralized bone. J Biomed Mater Res A, 2003, 67(3): 742-750.

1. Rho JY, Kuhn-Spearing L, Zioupos P. Mechanical properties and the hierarchical structure of bone. Med Eng Phys, 1998, 20(2): 92-102.
2. Swadener JG, Rho JY, Pharr GM. Effects of anisotropy on elastic moduli measured by nanoindentation in human tibial cortical bone. J Biomed Mater Res, 2001, 57(1): 108-112.
3. Novitskaya E, Chen PY, Lee S, et al. Anisotropy in the compressive mechanical properties of bovine cortical bone and the mineral and protein constituents. Acta Biomater, 2011, 7(8): 3170-3177.
4. Martin RB, Ishida J. The relative effects of collagen fiber orientation, porosity, density, and mineralization on bone strength. J Biomech, 1989, 22(5): 419-426.
5. Urist MR, Dawson E. Intertransverse process fusion with the aid of chemosterilized autolyzed antigen-extracted allogeneic (AAA) bone. Clin Orthop Relat Res, 1981, (154): 97-113.
6. Urist MR, Silverman BF, Büring K, et al. The bone induction principle. Clin Orthop Relat Res, 1967, (53): 243-283.
7. Bigham AS, Dehghani SN, Shafiei Z, et al. Xenogenic demineralized bone matrix and fresh autogenous cortical bone effects on experimental bone healing: radiological, histopathological and biomechanical evaluation. J Orthop Traumatol, 2008, 9(2): 73-80.
8. Mistry AS, Mikos AG. Tissue engineering strategies for bone regeneration. Adv Biochem Eng Biotechnol, 2005, 94: 1-22.
9. Sasso RC, LeHuec JC, Shaffrey C, et al. Iliac crest bone graft donor site pain after anterior lumbar interbody fusion: a prospective patient satisfaction outcome assessment. J Spinal Disord Tech, 2005, 18(Suppl): S77-81.
10. Silber JS, Anderson DG, Daffner SD, et al. Donor site morbidity after anterior iliac crest bone harvest for single-level anterior cervical discectomy and fusion. Spine (Phila Pa 1976), 2003, 28(2): 134-139.
11. Buck BE, Resnick L, Shah SM, et al. Human immunodeficiency virus cultured from bone. Implications for transplantation. Clin Orthop Relat Res, 1990, (251): 249-253.
12. Honsawek S, Bumrungpanichthaworn P, Thanakit V. Osteoinductive potential of small intestinal submucosa/demineralized bone matrix as composite scaffolds for bone tissue engineering. Asian Biomed, 2010, 4 (6): 913-922.
13. Guizzardi S, Di Silvestre M, Scandroglio R, et al. Implants of heterologous demineralized bone matrix for induction of posterior spinal fusion in rats. Spine (Phlia Pa 1976), 1992, 17(6): 701-707.
14. Pietrzak WS, Perns SV, Keyes J, et al. Demineralized bone matrix graft: a scientific and clinical case study assessment. J Foot Ankle Surg, 2005, 44(5): 345-353.
15. Honsawek S, Dhitiseith D, Phupong V. Gene expression characteristics of osteoblast differentiation in human umbilical cord mesenchymal stem cells induced by demineralized bone matrix. Asian Biomed, 2007, 1(4): 383-391.
16. Kasten P, Luginbühl R, van Griensven M, et al. Comparison of human bone marrow stromal cells seeded on calcium-deficient hydroxyapatite, beta-tricalcium phosphate and demineralized bone matrix. Biomaterials, 2003, 24(15): 2593-603.
17. Xie H, Yang F, Deng L, et al. The performance of a bone-derived scaffold material in the repair of critical bone defects in a rhesus monkey model. Biomaterials, 2007, 28(22): 3314-3324.
18. Honsawek S, Bumrungpanichthaworn P, Thitiset T, et al. Gene expression analysis of demineralized bone matrix-induced osteogenesis in human periosteal cells using cDNA array technology. Genet Mol Res, 2011, 10(3): 2093-2103.
19. Jackson DW, Simon TM, Lowery W, et al. Biologic remodeling after anterior cruciate ligament reconstruction using a collagen matrix derived from demineralized bone. An experimental study in the goat model. Am J Sports Med, 1996, 24(4): 405-414.
20. Yamada T. Bone-demineralized bone-bone graft for ligament reconstruction in rats. J Med Dent Sci, 2004, 51(1): 45-52.
21. Burstein AH, Zika JM, Heiple KG, et al. Contribution of collagen and mineral to the elastic-plastic properties of bone. J Bone Joint Surg (Am), 1975, 57(7): 956-961.
22. Paterson CR. Collagen chemistry and the brittle bone diseases. Endeavour, 1988, 12(2): 56-59.
23. Wang X, Bank RA, Tekoppele JM, et al. The role of collagen in determining bone mechanical properties. J Orthop Res, 2001, 19(6): 1021-1026.
24. 侯振德, 高瑞亭, 周欣竹. 干牛骨拉伸弹性模量沿径向的分布. 中国生物医学工程学报, 1995, 14(2): 149-154.
25. Bowman SM, Zeind J, Gibson LJ, et al. The tensile behavior of demineralized bovine cortical bone. J Biomech, 1996, 29(11): 1497-501.
26. Catanese J 3rd, Iverson EP, Ng RK, et al. Heterogeneity of the mechanical properties of demineralized bone. J Biomech, 1999, 32(12): 1365-1369.
27. Omae H, Zhao C, Sun YL, et al. Multilayer tendon slices seeded with bone marrow stromal cells: a novel composite for tendon engineering. J Orthop Res, 2009, 27(7): 937-942.
28. Mauney JR, Sjostorm S, Blumberg J, et al. Mechanical stimulation promotes osteogenic differentiation of human bone marrow stromal cells on 3-D partially demineralized bone scaffolds in vitro. Calcif Tissue Int, 2004, 74(5): 458-468.
29. 向强, 邓聪颖, 张远, 等. 成骨细胞特异性钙黏蛋白涂布脱钙骨基质材料对BMSCs 黏附及成骨分化能力的影响. 中国修复重建外科杂志, 2009, 23(5): 602-606.
30. Hou T, Li Q, Luo F, et al. Controlled dynamization to enhance reconstruction capacity of tissue-engineered bone in healing critically sized bone defects: an in vivo study in goats. Tissue Eng Part A, 2010, 16(1): 201-212.
31. 侯天勇, 罗飞, 刘杰, 等. 冻干组织工程骨与组织工程骨成骨活性比较研究. 中国修复重建外科杂志, 2010, 24(7): 779-784.
32. 杨军, 周振东, 李建军, 等. 动态压力促进骨基质支架中微血管形成的实验研究. 中华骨科杂志, 2011, 31(4): 372-378.
33. Pan Y, Dong SW, Hao Y, et al. Demineralized bone matrix gelatin as scaffold for tissue engineering. Afr J Microbiol Res, 2010, 4(9): 865-870.
34. Summitt MC, Reisinger KD. Characterization of the mechanical properties of demineralized bone. J Biomed Mater Res A, 2003, 67(3): 742-750.

Chinese Journal of Reparative and Reconstructive Surgery

TENSILE MECHANICAL CHARACTERISTICS OF DECALCIFIED CORTICAL BONE MATRIX

Abstract Full text Figures/Tables Video References Cited by

Format

Content