DIFFERENTIATION POTENTIAL AND APPLICATION OF STEM CELLS FROM ADIPOSE TISSUE_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

SHI Linli ¹ , YANG Xiangqun ²

1No.2 Clinical Company, Graduate Management Team, 2Department of Anatomy, Institute of Biomedical Engineering, Second Military Medical University, Shanghai, 200433, P.R.China. Corresponding author: YANG Xiangqun, E-mail: yangxq@smmu.edu.cn;

Corresponding author：

Keywords：

Adipose-derived stem cells; Dedifferentiated fat cells; Stromal vascular fraction; Regenerative medicine

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Objective To introduce types and differentiation potentials of stem cells from adipose tissue, and its applications on regenerative medicine and advantages. Methods The literature of original experimental study and clinical research about bone marrow mesenchymal stem cells (BMSCs), adipose-derived stem cells (ADSCs), and dedifferentiated fat (DFAT) cells was extensively reviewed and analyzed. Results ADSCs can be isolated from stromal vascular fraction. As ADSCs have multi-lineage potentials, such as adipogenesis, osteogenesis, chondrogenesis, angiogenesis, myogenesis, and neurogenesis, they have already been successfully used in regenerative medicine areas. Dramatically, mature fat cells can be dedifferentiated and changed into fibroblast-like cells, named DFAT cells, via ceiling culture method. DFAT cells also had the same multi-lineage potentials as ADSCs, differentiating into adipocytes, osteocytes, chondrocytes, endothelial cells, muscle cells, and nerve cells. Compared with BMSCs which are commonly used as adult stem cells, ADSCs and DFAT cells have extensive sources and can be easily acquired. While compared with ADSCs, DFAT cells have good homogeneity and b proliferation capacity. Conclusion As a potential source of stem cells, adipose tissue will provide a new promising for regenerative medicine.

Citation： SHI Linli,YANG Xiangqun. DIFFERENTIATION POTENTIAL AND APPLICATION OF STEM CELLS FROM ADIPOSE TISSUE. Chinese Journal of Reparative and Reconstructive Surgery, 2012, 26(8): 1007-1011. doi: Copy

1.	Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell based therapied. Tissue Eng, 2001, 7(2): 211-228．.
2.	Schächinger V, Erbs S, Elsässer A, et al. Improved clinical outcome after intracoronary administration of bone marrow-derived progenitor cells in acute myocardial infarction: final 1-year results of the REPAIR-AMI trial. Eur Heart J, 2006, 27(23): 2775-2783．.
3.	Chen J, Larsson L, Haugen E, et al. Effects of autoantibodies removed by immunoadsorption from patients with dilated cardiomyopathy on neonatal rat cardiomyocytes. Eur J Heart Fail, 2006, 8(5): 460-467.
4.	Suga H, Matsumoto D, Inoue K, et al. Numerical measurement of viable and nonviable adipocytes and other cellular components in aspirated fat tissue. Plast Reconstr Surg, 2008, 122(1): 103-114.
5.	Garcia-Olmo D, Herreros D, Pascual M, et al. Treatment of enterocutaneous fistula in Crohn’s Disease with adipose-derived stem cells: a comparison of protocols with and without cell expansion. Int J Colorectal Dis, 2009, 24(1): 27-30.
6.	Riordan NH, Ichim TE, Min WP, et al. Non-expanded adipose stromal vascular fraction cell therapy for multiple sclerosis. J Transl Med, 2009, 7: 29.
7.	梁星, 朱辉. 脂肪来源干细胞的应用研究进展. 中国美容医学, 2010, 19(11): 1734-1737.
8.	Wickham MQ, Erickson GR, Gimble JM, et al. Multipotent stromal cells derived from the infrapatellar fat pad of the knee. Clin Orthop Relat Res, 2003, (412): 196-212.
9.	Al-Salleeh F, Beatty MW, Reinhardt RA, et al. Human osteogenic protein-1 induces osteogenic differentiation of adipose-derived stem cells harvested from mice. Arch Oral Biol, 2008, 53(10): 928-936.
10.	Lendeckel S, Jödicke A, Christophis P, et al. Autologous steam cells (adipose) and fibrin glue used to treat wide spread traumatic calvarial defect: case report. J Craniomaxillofac Surg, 2004, 32(6): 370-373.
11.	Jin X, Sun Y, Zhang K, et al. Ectopic neocartilage formation from predifferentiated human adipose derived stem cells induced by adenoviral-mediated transfer of hTGF beta2. Biomaterials, 2007, 28(19): 2994-3003.
12.	Afizah H, Yang Z, Hui JH, et al. A comparison between the chendrngenic potential of human bone marrow stem cells(BMSCs)and adipose-derived stem cells(ADSCs) taken from same donor. Tissue Eng, 2007, 13(4): 659-666.
13.	Cao Y, Sun Z, Liao L, et al. Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo. Biochem Biophys Res Commun, 2005, 332(2): 370-379.
14.	Planat-Bénard V, Silvestre JS, Cousin B, et al. Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives. Circulation, 2004, 109(5): 656-663.
15.	Schenke-Layland K, Strem BM, Jordan MC, et al. Adipose tissue-derived cells improve cardiac function following myocardial infarction. J Surg Res, 2009, 153(2): 217-223.
16.	Planat-Bénard V, Menard C, André M, et al. Spontaneous cardiomyocyte differentiation from adipose tissue stroma cells. Circ Res, 2004, 94(2): 223-229.
17.	Miyahara Y, Nagaya N, Kataoka M, et al. Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction. Nat Med, 2006, 12(4): 459-465.
18.	Vieira NM, Brandalise V, Zucconi E, et al. Human multipotent adipose-derived stem cells restore dystrophin expression of Duchenne skeletal-muscle cells in vivo. Biol Cell, 2008, 100(4): 231-241.
19.	王刚, 曹卫刚. 具有多向分化潜能的脂肪干细胞研究的新进展. 组织工程与重建外科杂志, 2009, 5(3): 173-176.
20.	Sugihara H, Yonemitsu N, Miyabara S, et al. Primary cultures of unilocular fat cells: characteristics of growth in vitro and changes in differentiation properties. Differentiation, 1986, 31(1): 42-49.
21.	Matsumoto T, Kano K, Kondo D, et al. Mature adipocyte- derived dedifferentiated fat cells exhibit multilineage potential. J Cell Physiol, 2008, 215(1): 210-222.
22.	Shen J, Sugawara A, Yamashita J, et al. Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues. Int J Oral Sci, 2011, 3(3): 117-124.
23.	Yagi K, Kondo D, Okazaki Y, et al. A novel preadipocyte cell line established from mouse adult mature adipocytes. Biochem Biophys Res Commun, 2004, 321(4): 967-974.
24.	Nobusue H, Endo T, Kano K, et al. Establishment of a preadipocyte cell line derived from mature adipocytes of GFP transgenic mice and formation of adipose tissue. Cell Tissue Res, 2008, 332(3): 435-446.
25.	Jumabay M, Zhang R, Yao Y, et al. Spontaneously beating cardiomyocytes derived from white mature adipocytes. Cardiovas Res, 2010, 85(1): 17-27.
26.	Obinata D, Matsumoto T, Ikado Y, et al. Transplantation of mature adipocyte-derived dedifferentiated fat (DFAT) cells improves urethral sphincter contractility in a rat model. Int J Urol, 2011, 18(12): 827-834.
27.	Jumabay M, Matsumoto T, Yokoyama S, et al. Dedifferentiated fat cells convert to cardiomyocyte phenotype and repair infarcted cardiac tissue in rats. J Mol Cell Cardiol, 2009, 47(5): 565-575.
28.	Ohta Y, Takenaga M, Tokura Y, et al. Mature adipocyte-derived cells, dedifferentiated fat cells (DFAT), promoted functional recovery from spinal cord injury-induced motor dysfunction in rats. Cell Transplant, 2008, 17(8): 877-886.
29.	李春明, 刘毅. 体外的培养的人脂肪间充质干细胞生物学特性的研究. 中国美容医学, 2007, 16(2): 159-162.
30.	朱希山, 施薇, 台卫平, 等. 脂肪与骨髓来源间充质干细胞生物学特性的比较. 中国组织工程研究与临床康复, 2011, 15(32): 5937-5940.
31.	周进, 田国萍, 王静娥, 等. 脂肪基质干细胞和骨髓基质干细胞的体外分化能力. 中国组织工程研究与临床康复, 2011, 15(32): 5932-5935.
32.	Al-Nbaheen M, Vishnubalaji R, Ali D, et al. Human stromal(mesenchymal) stem cells from bone marrow, adipose tissue and skin exhibit differences in molecular phenotype and differentiation potential. Stem Cell Rev, 2012. [Epub ahead of print].
33.	Lee JM, Jung J, Lee HJ, et al. Comparison of immunomodulatory effects of placenta stem cells with bone marrow and adipose mesenchymal stem cells. Int Immunopharmacol, 2012, 13(2): 219-224.
34.	朱希山, 台卫平, 施薇, 等. 骨髓和脂肪来源间充质干细胞的免疫调节作用. 中国组织工程研究与临床康复, 2011, 15(36): 6683-6686.
35.	Zhu Y, Liu T, Song K, et al. Adipose-derived stem cell: a better stem cell than BMSC. Cell Biochem Funct, 2008, 26(6): 664-675.
36.	Gao Q, Zhao L, Song Z, et al. Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs. Mol Biol Rep, 2012, 39(5): 5791-5804.

1. Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell based therapied. Tissue Eng, 2001, 7(2): 211-228．.
2. Schächinger V, Erbs S, Elsässer A, et al. Improved clinical outcome after intracoronary administration of bone marrow-derived progenitor cells in acute myocardial infarction: final 1-year results of the REPAIR-AMI trial. Eur Heart J, 2006, 27(23): 2775-2783．.
3. Chen J, Larsson L, Haugen E, et al. Effects of autoantibodies removed by immunoadsorption from patients with dilated cardiomyopathy on neonatal rat cardiomyocytes. Eur J Heart Fail, 2006, 8(5): 460-467.
4. Suga H, Matsumoto D, Inoue K, et al. Numerical measurement of viable and nonviable adipocytes and other cellular components in aspirated fat tissue. Plast Reconstr Surg, 2008, 122(1): 103-114.
5. Garcia-Olmo D, Herreros D, Pascual M, et al. Treatment of enterocutaneous fistula in Crohn’s Disease with adipose-derived stem cells: a comparison of protocols with and without cell expansion. Int J Colorectal Dis, 2009, 24(1): 27-30.
6. Riordan NH, Ichim TE, Min WP, et al. Non-expanded adipose stromal vascular fraction cell therapy for multiple sclerosis. J Transl Med, 2009, 7: 29.
7. 梁星, 朱辉. 脂肪来源干细胞的应用研究进展. 中国美容医学, 2010, 19(11): 1734-1737.
8. Wickham MQ, Erickson GR, Gimble JM, et al. Multipotent stromal cells derived from the infrapatellar fat pad of the knee. Clin Orthop Relat Res, 2003, (412): 196-212.
9. Al-Salleeh F, Beatty MW, Reinhardt RA, et al. Human osteogenic protein-1 induces osteogenic differentiation of adipose-derived stem cells harvested from mice. Arch Oral Biol, 2008, 53(10): 928-936.
10. Lendeckel S, Jödicke A, Christophis P, et al. Autologous steam cells (adipose) and fibrin glue used to treat wide spread traumatic calvarial defect: case report. J Craniomaxillofac Surg, 2004, 32(6): 370-373.
11. Jin X, Sun Y, Zhang K, et al. Ectopic neocartilage formation from predifferentiated human adipose derived stem cells induced by adenoviral-mediated transfer of hTGF beta2. Biomaterials, 2007, 28(19): 2994-3003.
12. Afizah H, Yang Z, Hui JH, et al. A comparison between the chendrngenic potential of human bone marrow stem cells(BMSCs)and adipose-derived stem cells(ADSCs) taken from same donor. Tissue Eng, 2007, 13(4): 659-666.
13. Cao Y, Sun Z, Liao L, et al. Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo. Biochem Biophys Res Commun, 2005, 332(2): 370-379.
14. Planat-Bénard V, Silvestre JS, Cousin B, et al. Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives. Circulation, 2004, 109(5): 656-663.
15. Schenke-Layland K, Strem BM, Jordan MC, et al. Adipose tissue-derived cells improve cardiac function following myocardial infarction. J Surg Res, 2009, 153(2): 217-223.
16. Planat-Bénard V, Menard C, André M, et al. Spontaneous cardiomyocyte differentiation from adipose tissue stroma cells. Circ Res, 2004, 94(2): 223-229.
17. Miyahara Y, Nagaya N, Kataoka M, et al. Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction. Nat Med, 2006, 12(4): 459-465.
18. Vieira NM, Brandalise V, Zucconi E, et al. Human multipotent adipose-derived stem cells restore dystrophin expression of Duchenne skeletal-muscle cells in vivo. Biol Cell, 2008, 100(4): 231-241.
19. 王刚, 曹卫刚. 具有多向分化潜能的脂肪干细胞研究的新进展. 组织工程与重建外科杂志, 2009, 5(3): 173-176.
20. Sugihara H, Yonemitsu N, Miyabara S, et al. Primary cultures of unilocular fat cells: characteristics of growth in vitro and changes in differentiation properties. Differentiation, 1986, 31(1): 42-49.
21. Matsumoto T, Kano K, Kondo D, et al. Mature adipocyte- derived dedifferentiated fat cells exhibit multilineage potential. J Cell Physiol, 2008, 215(1): 210-222.
22. Shen J, Sugawara A, Yamashita J, et al. Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues. Int J Oral Sci, 2011, 3(3): 117-124.
23. Yagi K, Kondo D, Okazaki Y, et al. A novel preadipocyte cell line established from mouse adult mature adipocytes. Biochem Biophys Res Commun, 2004, 321(4): 967-974.
24. Nobusue H, Endo T, Kano K, et al. Establishment of a preadipocyte cell line derived from mature adipocytes of GFP transgenic mice and formation of adipose tissue. Cell Tissue Res, 2008, 332(3): 435-446.
25. Jumabay M, Zhang R, Yao Y, et al. Spontaneously beating cardiomyocytes derived from white mature adipocytes. Cardiovas Res, 2010, 85(1): 17-27.
26. Obinata D, Matsumoto T, Ikado Y, et al. Transplantation of mature adipocyte-derived dedifferentiated fat (DFAT) cells improves urethral sphincter contractility in a rat model. Int J Urol, 2011, 18(12): 827-834.
27. Jumabay M, Matsumoto T, Yokoyama S, et al. Dedifferentiated fat cells convert to cardiomyocyte phenotype and repair infarcted cardiac tissue in rats. J Mol Cell Cardiol, 2009, 47(5): 565-575.
28. Ohta Y, Takenaga M, Tokura Y, et al. Mature adipocyte-derived cells, dedifferentiated fat cells (DFAT), promoted functional recovery from spinal cord injury-induced motor dysfunction in rats. Cell Transplant, 2008, 17(8): 877-886.
29. 李春明, 刘毅. 体外的培养的人脂肪间充质干细胞生物学特性的研究. 中国美容医学, 2007, 16(2): 159-162.
30. 朱希山, 施薇, 台卫平, 等. 脂肪与骨髓来源间充质干细胞生物学特性的比较. 中国组织工程研究与临床康复, 2011, 15(32): 5937-5940.
31. 周进, 田国萍, 王静娥, 等. 脂肪基质干细胞和骨髓基质干细胞的体外分化能力. 中国组织工程研究与临床康复, 2011, 15(32): 5932-5935.
32. Al-Nbaheen M, Vishnubalaji R, Ali D, et al. Human stromal(mesenchymal) stem cells from bone marrow, adipose tissue and skin exhibit differences in molecular phenotype and differentiation potential. Stem Cell Rev, 2012. [Epub ahead of print].
33. Lee JM, Jung J, Lee HJ, et al. Comparison of immunomodulatory effects of placenta stem cells with bone marrow and adipose mesenchymal stem cells. Int Immunopharmacol, 2012, 13(2): 219-224.
34. 朱希山, 台卫平, 施薇, 等. 骨髓和脂肪来源间充质干细胞的免疫调节作用. 中国组织工程研究与临床康复, 2011, 15(36): 6683-6686.
35. Zhu Y, Liu T, Song K, et al. Adipose-derived stem cell: a better stem cell than BMSC. Cell Biochem Funct, 2008, 26(6): 664-675.
36. Gao Q, Zhao L, Song Z, et al. Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs. Mol Biol Rep, 2012, 39(5): 5791-5804.

Chinese Journal of Reparative and Reconstructive Surgery

DIFFERENTIATION POTENTIAL AND APPLICATION OF STEM CELLS FROM ADIPOSE TISSUE

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