• State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, P.R.China. Corresponding author: CAI Haibo, E-mail: caihaibo@ecust.edu. cn;
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ObjectiveTo investigate the relationship between the expressions of B-cell-specific monoclonal leukemia virus insert site 1 (Bmi1) and human telomerase reverse transcriptase (hTERT) genes and the proliferative capacity of stem cells. MethodsCord blood CD34+ cells were cultured in IMDM medium containing fetal bovine serum, stem cell growth factor, thrombopoietin, and Fms-like tyrosine kinase 3 ligand during a 28-day ex vivo expansion process, while the expansion fold, specific growth rate, and the colony-forming efficiency were monitored. Then the Bmi1 and hTERT mRNA expressions in CD34+ cells were detected by fluorescence quantitative PCR, and the relations between the expressions and the cell proliferative capacity were analyzed. ResultsCD34+ cells expanded (20.1 ± 3.5) folds during the 28-day culture, while the proportion declined from 95.5% ± 2.6% before culture to 2.1% ± 0.4%. Both the specific growth rate and colony-forming efficiency of CD34+ cells declined significantly after 7 days, while the expression levels of Bmi1 and hTERT mRNA in CD34+ cells reached top at 7 days and declined gradually thereafter. ConclusionThe expressions of Bmi1 and hTERT genes may have a close relation to the proliferative capacity of CD34+ cells.

Citation: GE Jianyun,CAI Haibo,DU Zheng,TAN Wensong.. EXPRESSIONS OF B-CELL-SPECIFIC MONOCLONAL LEUKEMIA VIRUS INSERT SITE 1 AND HUMAN TELOMERASE REVERSE TRANSCRIPTASE GENES IN CD34+ CELLS DURING EX VIVO EXPANSION PROCESS. Chinese Journal of Reparative and Reconstructive Surgery, 2012, 26(9): 1112-1116. doi: Copy