RESEARCH PROGRESS OF CELL DEDIFFERENTIATION INDUCED BY reversine_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

HU Xuqi , ZHANG Xiaolei , ZHOU Yifei , YU Yang , XU Huazi.

Department of Orthopaedics, the Second Affiliated Hospital of Wenzhou Medical College, Wenzhou Zhejiang, 325000, P.R.China. Corresponding author: XU Huazi, E-mail: spinexu@163.com;

Corresponding author：

Keywords：

Pluripotent stem cells; Reversine; Dedifferentiation; Reprogram

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ObjectiveTo review the research progress of different ways of stem cells generation and cells dedifferentiation induced by reversine. MethodsThe papers related to reversine inducing cells dedifferentiation and stem cells generation were reviewed. ResultsTo obtain stem cells, there are always some disadvantages via somatic cell nuclear transfer or gene transfection. However reversine, a small molecule, can induce cells dedifferentiation which has unique advantage. But its mechanism is still unclear. ConclusionThe chemical approach for generation of induced pluripotent stem cells by reversine may take the place of other methods.

Citation： HU Xuqi,ZHANG Xiaolei,ZHOU Yifei,YU Yang,XU Huazi.. RESEARCH PROGRESS OF CELL DEDIFFERENTIATION INDUCED BY reversine. Chinese Journal of Reparative and Reconstructive Surgery, 2012, 26(9): 1126-1129. doi: Copy

1.	Morrison SJ, Kimble J. Asymmetric and symmetric stem-cell divisions in development and cancer. Nature, 2006, 441(7097): 1068-1074.
2.	Anastasia L, Piccoli M, Garatti A, et al. Cell reprogramming: a new chemical approach to stem cell biology and tissue regeneration. Curr Pharm Biotechnol, 2011, 12(2): 146-150.
3.	Anastasia L, Pelissero G, Venerando B, et al. Cell reprogramming: expectations and challenges for chemistry in stem cell biology and regenerative medicine. Cell Death Differ, 2010, 17(8): 1230-1237.
4.	Campbell KH, McWhir J, Ritchie WA, et al. Sheep cloned by nuclear transfer from a cultured cell line. Nature, 1996, 380(6569): 64-66.
5.	Cowan CA, Atienza J, Melton DA, et al. Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells. Science, 2005, 309(5739): 1369-1373.
6.	Tada M, Takahama Y, Abe K, et al. Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells. Curr Biol, 2001, 11(19): 1553-1558.
7.	Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 2006, 126(4): 663-676.
8.	Lowry WE, Richter L, Yachechko R, et al. Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc Natl Acad Sci U S A, 2008, 105(8): 2883-2888.
9.	Okita K, Ichisaka T, Yamanaka S. Generation of germline-competent induced pluripotent stem cells. Nature, 2007, 448(7151): 313-317.
10.	Mochiduki Y, Okita K. Methods for iPS cell generation for basic research and clinical applications. Biotechnology J, 2012, 7(6): 789-797.
11.	Bain G, Kitchens D, Yao M, et al. Embryonic stem cells express neuronal properties in vitro. Dev Biol, 1995, 168(2): 342-357.
12.	Christman JK. 5-Azacytidine and 5-aza-2’-deoxycytidine as inhibitors of DNA methylation: mechanistic studies and their implications for cancer therapy. Oncogene, 2002, 21(35): 5483-5495.
13.	Esteban MA, Wang T, Qin BM, et al. Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. Cell Stem Cell, 2010, 6(1): 71-79.
14.	Chen S, Zhang Q, Wu X, et al. Dedifferentiation of lineage-committed cells by a small molecule. J Am Chem Soc, 2004, 126(2): 410-411.
15.	Chen S, Takanashi S, Zhang Q, et al. Reversine increases the plasticity of lineage-committed mammalian cells. Proc Natl Acade Sci U S A, 2007, 104(25): 10482-10487.
16.	Anastasia L, Sampaolesi M, Papini N, et al. Reversine-treated fibroblasts acquire myogenic competence in vitro and in regenerating skeletal muscle. Cell Death Differ, 2006, 13(12): 2042-2051.
17.	Saraiya M, Nasser R, Zeng Y, et al. Reversine enhances generation of progenitor-like cells by dedifferentiation of annulus fibrosus cells. Tissue Eng Part A, 2010, 16(4): 1443-1455.
18.	Lee EK, Bae GU, You JS, et al. Reversine increases the plasticity of lineage-committed cells toward neuroectodermal lineage. J Biol Chem, 2009, 284(5): 2891-2901.
19.	Bellefroid EJ, Kobbe A, Gruss P, et al. Xiro3 encodes a Xenopus homolog of the Drosophila Iroquois genes and functions in neural specification. EMBO J, 1998, 17(1): 191-203.
20.	Basch ML, Bronner-Fraser M, García-Castro MI. Specification of the neural crest occurs during gastrulation and requires Pax7. Nature, 2006, 441(7090): 218-222.
21.	Kageyama R, Ohtsuka T, Kobayashi T. The Hes gene family: repressors and oscillators that orchestrate embryogenesis. Development, 2007, 134(7): 1243-1251.
22.	Kim YK, Choi HY, Kim NH, et al. Reversine stimulates adipocyte differentiation and downregulates Akt and p70 (s6k) signaling pathways in 3T3-L1 cells. Biochem Biophys Res Commun, 2007, 358(2): 553-558.
23.	D’Alise AM, Amabile G, Iovino M, et al. Reversine, a novel Aurora kinases inhibitor, inhibits colony formation of human acute myeloid leukemia cells. Mol Cancer Ther, 2008, 7(5): 1140-1149.
24.	Vader G, Cruijsen CW, van Harn T, et al. The chromosomal passenger complex controls spindle checkpoint function independent from its role in correcting microtubule-kinetochore interactions. Mol Biol Cell, 2007, 18(11): 4553-4564.
25.	Musacchio A, Salmon ED. The spindle-assembly checkpoint in space and time. Nat Rev Mol Cell Biol, 2007, 8(5): 379-393.
26.	Amabile G, D’Alise AM, Iovino M, et al. The Aurora B kinase activity is required for the maintenance of the differentiated state of murine myoblasts. Cell Death Differ, 2009, 16(2): 321-330.
27.	Santaguida S, Tighe A, D’Alise AM, et al. Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine. J Cell Biol, 2010, 190(1): 73-87.
28.	Jung DW, Williams DR. Novel chemically defined approach to produce multipotent cells from terminally differentiated tissue syncytia. ACS Chem Biol, 2011, 6(6): 553-562.

1. Morrison SJ, Kimble J. Asymmetric and symmetric stem-cell divisions in development and cancer. Nature, 2006, 441(7097): 1068-1074.
2. Anastasia L, Piccoli M, Garatti A, et al. Cell reprogramming: a new chemical approach to stem cell biology and tissue regeneration. Curr Pharm Biotechnol, 2011, 12(2): 146-150.
3. Anastasia L, Pelissero G, Venerando B, et al. Cell reprogramming: expectations and challenges for chemistry in stem cell biology and regenerative medicine. Cell Death Differ, 2010, 17(8): 1230-1237.
4. Campbell KH, McWhir J, Ritchie WA, et al. Sheep cloned by nuclear transfer from a cultured cell line. Nature, 1996, 380(6569): 64-66.
5. Cowan CA, Atienza J, Melton DA, et al. Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells. Science, 2005, 309(5739): 1369-1373.
6. Tada M, Takahama Y, Abe K, et al. Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells. Curr Biol, 2001, 11(19): 1553-1558.
7. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 2006, 126(4): 663-676.
8. Lowry WE, Richter L, Yachechko R, et al. Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc Natl Acad Sci U S A, 2008, 105(8): 2883-2888.
9. Okita K, Ichisaka T, Yamanaka S. Generation of germline-competent induced pluripotent stem cells. Nature, 2007, 448(7151): 313-317.
10. Mochiduki Y, Okita K. Methods for iPS cell generation for basic research and clinical applications. Biotechnology J, 2012, 7(6): 789-797.
11. Bain G, Kitchens D, Yao M, et al. Embryonic stem cells express neuronal properties in vitro. Dev Biol, 1995, 168(2): 342-357.
12. Christman JK. 5-Azacytidine and 5-aza-2’-deoxycytidine as inhibitors of DNA methylation: mechanistic studies and their implications for cancer therapy. Oncogene, 2002, 21(35): 5483-5495.
13. Esteban MA, Wang T, Qin BM, et al. Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. Cell Stem Cell, 2010, 6(1): 71-79.
14. Chen S, Zhang Q, Wu X, et al. Dedifferentiation of lineage-committed cells by a small molecule. J Am Chem Soc, 2004, 126(2): 410-411.
15. Chen S, Takanashi S, Zhang Q, et al. Reversine increases the plasticity of lineage-committed mammalian cells. Proc Natl Acade Sci U S A, 2007, 104(25): 10482-10487.
16. Anastasia L, Sampaolesi M, Papini N, et al. Reversine-treated fibroblasts acquire myogenic competence in vitro and in regenerating skeletal muscle. Cell Death Differ, 2006, 13(12): 2042-2051.
17. Saraiya M, Nasser R, Zeng Y, et al. Reversine enhances generation of progenitor-like cells by dedifferentiation of annulus fibrosus cells. Tissue Eng Part A, 2010, 16(4): 1443-1455.
18. Lee EK, Bae GU, You JS, et al. Reversine increases the plasticity of lineage-committed cells toward neuroectodermal lineage. J Biol Chem, 2009, 284(5): 2891-2901.
19. Bellefroid EJ, Kobbe A, Gruss P, et al. Xiro3 encodes a Xenopus homolog of the Drosophila Iroquois genes and functions in neural specification. EMBO J, 1998, 17(1): 191-203.
20. Basch ML, Bronner-Fraser M, García-Castro MI. Specification of the neural crest occurs during gastrulation and requires Pax7. Nature, 2006, 441(7090): 218-222.
21. Kageyama R, Ohtsuka T, Kobayashi T. The Hes gene family: repressors and oscillators that orchestrate embryogenesis. Development, 2007, 134(7): 1243-1251.
22. Kim YK, Choi HY, Kim NH, et al. Reversine stimulates adipocyte differentiation and downregulates Akt and p70 (s6k) signaling pathways in 3T3-L1 cells. Biochem Biophys Res Commun, 2007, 358(2): 553-558.
23. D’Alise AM, Amabile G, Iovino M, et al. Reversine, a novel Aurora kinases inhibitor, inhibits colony formation of human acute myeloid leukemia cells. Mol Cancer Ther, 2008, 7(5): 1140-1149.
24. Vader G, Cruijsen CW, van Harn T, et al. The chromosomal passenger complex controls spindle checkpoint function independent from its role in correcting microtubule-kinetochore interactions. Mol Biol Cell, 2007, 18(11): 4553-4564.
25. Musacchio A, Salmon ED. The spindle-assembly checkpoint in space and time. Nat Rev Mol Cell Biol, 2007, 8(5): 379-393.
26. Amabile G, D’Alise AM, Iovino M, et al. The Aurora B kinase activity is required for the maintenance of the differentiated state of murine myoblasts. Cell Death Differ, 2009, 16(2): 321-330.
27. Santaguida S, Tighe A, D’Alise AM, et al. Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine. J Cell Biol, 2010, 190(1): 73-87.
28. Jung DW, Williams DR. Novel chemically defined approach to produce multipotent cells from terminally differentiated tissue syncytia. ACS Chem Biol, 2011, 6(6): 553-562.

Chinese Journal of Reparative and Reconstructive Surgery

RESEARCH PROGRESS OF CELL DEDIFFERENTIATION INDUCED BY reversine

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