YE Lianhua 1 , HUANG Yunchao 1 , XU Geng 1 , YANG Dakuan 2 , LIU Xin 3 , ZHOU Youquan 4 , GUO Fengli 4
  • 1Department of Thoracic Surgery, Lung Cancer Research Centre of the Cancer Institute of Yunnan Province, 3Laboratory of Molecular Biology, Cancer Institute of Yunnan Province, 4Bacteria Room, Department of Clinical Laboratory, the Third Affiliated Hospital, Kunming Medical College, Tumor Hospital of unnan Province, Kunming Yunnan, 650118, P.R.China;;
  • 2Department of Thoracic Surgery, the Second Affiliated Hospital, Kunming Medical College.;
Iatrogenic; Staphylococcus epidermidis; Intercellar adhesion operon; Bacterial biofilm; Polyvinyl chloride
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Objective The intercellular adhesion (ica) gene of Staphylococcus epidermidis (SE) is a key factor to bacterial aggregation, to analysis the genotype of iatrogenic SE and to explore the effect of iatrogenic SE ica operon on the
formation of bacterial biofilm on the surface of polyvinyl chloride (PVC). Methods Fifty-six cl inical isolates of iatrogenic SE
were selected, and PCR and gene sequencing were used to detect the genes related with bacterial biofilm formation. The genes contained 16S rRNA, autolysin (atlE), fibrinogen binding protein (fbe), and icaADB. The bacteria suspension of 1 × 105 cfu/mL iatrogenic SE was prepared; according to the test results of target genes, the PVC material and the genotype of icaADB+, atlE+, fbe+ strains were co-cultivated as the ica positive group; the PVC material and the genotype of icaADB-, atlE+, fbe+ strains were co-cultivated as the ica negative group. The thickness of biofilm and bacterial community quantity unit area on PVC materials were measured by confocal laser scanning microscope, and the surface structure of biofilm formation was observed by scanning electron microscope (SEM) at 6, 12, 18, 24, and 30 hours. Results The positive rate of 16S rRNA of iatrogenic SE strains was 100% (56/56). The genotype of icaADB+, atlE+, and fbe+ strains accounted for 57.1% (32/56). The genotype of icaADB-, atlE+, and fbe+ strains accounted for 37.5% (21/56). The sequencing results showed that the product sequences of 16S rRNA, atlE, fbe, and icaADB were consistent with those in GenBank. With time, no significant bacterial biofilm formed on the surface of PVC in ica operon negative group. But in ica operon positive group, the number of bacterial community was gradually increased, and the volume of bacterial biofilms was gradually increased on the surface of PVC. At 24 hours, mature bacterial biofilm structure formed, and at 30 hours, the volume of bacterial biofilms was tending towards stabil ity. The thickness of biofilm (F=6 714.395, P=0.000) and the bacterial community quantity unit area on PVC materials (F=435.985, P=0.000) in ica operon positive group
were significantly higher than those in ica operon negative group. Conclusion Iatrogenic SE can be divided into 2 types of
ica operon negative and ica operon positive bacteria. The iatrogenic SE ica operon can strengthen bacterium biofilm formation capabil ity on PVC materials, bacterium community quantity, and thickness of biofilm, it plays an important role in bacterium biofilm formation on PVC materials.

Citation： YE Lianhua,HUANG Yunchao,XU Geng,YANG Dakuan,LIU Xin,ZHOU Youquan,GUO Fengli. EFFECT OF IATROGENIC STAPHYLOCOCCUS EPIDERMIDIS INTERCELLAR ADHESION OPERON ON FORMATION OF BACTERIAL BIOFILM ON SURFACE OF POLYVINYL CHLORIDE. Chinese Journal of Reparative and Reconstructive Surgery, 2011, 25(4): 466-471. doi: Copy