Objective To assess the effect of basic fibroblast growth factor (bFGF) and 5-fluorouracil (5-FU) appl ied topically on the tendon adhesion and the heal ing process after the flexor tendon repair in Leghorn chickens. Methods Ninety male Leghorn chickens (weighing 3.0-3.5 kg) were randomly divided into 3 groups, with 30 chickens in each group. The flexor
digitorum profundus tendons of the third right toes were transected and sutured directly. The repair site in group A was given 0.6 μL fibrin sealant (FS). In group B, the repair site was given 0.6 μL FS containing 500 ng bFGF. In group C, before the tendons were transected, they had been soaked in 5-FU solution, and then the same treatment as group B was given. Six specimens of the third toe were harvested to perform the macroscopical and histological examinations at 1, 2, 4, and 8 weeks, respectively, and to perform the biomechanical test at 8 weeks. Results All animals survived until the experiment was completed. All incisions healed smoothly. No rupture occurred in the reparied tendon. At 8 weeks, the adhesion degree was l ighter in group C than in group B (P  lt; 0.05), but there was no significant difference in the adhesion degree between group A and groups B, C (P  gt; 0.05). At 1, 2, and 4 weeks after operation, the number of fibroblast cells of group A was significantly less than that of group B (P  lt; 0.05), and the number of fibroblast cells of group C was significantly less than that of group A and group B in the tendon sheath and epitenon (P  lt; 0.05); however, it was significantly more than that of group A in the tendon parenchyma (P  lt; 0.05), and no significant difference was observed when compared with that of group B (P  gt; 0.05). At 8 weeks, no difference was found among 3 groups (P  gt; 0.05). The collagen fiber content of group A was significantly less than that of group B at 4 and 8 weeks (P  lt; 0.05). In the sheath and epitenon, the collagen fiber content of group A was significantly more than that of group C at 4 weeks (P  lt; 0.05); however, no significant difference was found between 2 groups at 8 weeks (P  gt; 0.05). The collagen fiber content of group A was
significantly less than that of group C in the parenchyma at 4 and 8 weeks (P  lt; 0.05). At all time points, the collagen fiber content of group B was significantly more than that of group C in the sheath and epitenon (P  lt; 0.05), but no significant difference in the parenchyma was observed between 2 groups (P  gt; 0.05). The biomechanical tests showed that the gl iding excursion of the tendon in groups A, B, and C was (3.51 ± 0.56), (2.84 ± 0.42), and (4.56 ± 0.59) mm, respectively; the work of flexion was (14.08 ± 1.85), (20.62 ± 3.52), and (10.91 ± 1.53) N.mm, respectively; and the ultimate tensile strength of the tendon was (11.26 ± 1.83), (15.02 ± 2.20), and (14.40 ± 1.57) N, respectively. There were significant differences in the gl iding excursion of the tendon and the work of flexion among 3 groups (P  lt; 0.05) and in the ultimate tensile strength of the tendon between group A and groups B, C (P  lt; 0.05), but there was no significant difference in the ultimate tensile strength of the tendon between group B and group C (P  gt; 0.05). Conclusion Local single-use bFGF and 5-FU can not only effectively promote the heal ing of flexor tendon, but also significantly reduce tendon adhesion.

Citation： SHENG Jiagen,ZENG Bingfang,JIANG Peizhu,FAN Cunyi.. EFFECT OF LOCAL BASIC FIBROBLAST GROWTH FACTOR AND 5-FLUOROURACIL ON ACCELERATING HEALING AND PREVENTING TENDON ADHESION AFTER FLEXOR TENDON REPAIR. Chinese Journal of Reparative and Reconstructive Surgery, 2011, 25(6): 711-717. doi: Copy