Objective Construction of viable tissue engineered bone is one of the most important research fields in the cl inical appl ication of bone tissue engineering, to investigate the function of nerve factors in bone tissue engineering by cell
detection in vitro and construction of neurotization tissue engineered bone in vivo. Methods Fifty-four healthy New Zealand
white rabbits, male or female, weighing 2-3 kg, were involved in this study. Bone marrow mesenchymal stem cells (BMSCs) from the bone marrow of white rabbits were cultured. The second passage of BMSCs were treated with sensory nerve or motor nerve homogenates, using the LG-DMEM complete medium as control. The prol iferation and osteogenic differentiation of the cells were observed and tested by the MTT assay, alkal ine phosphatase (ALP) stain, and collagen type I immunocytochemistry identification. The osteogenic induced BMSCs were inoculated in β tricalcium phosphate (β-TCP) biomaterial scaffold and cultured for 72 hours, then the β-TCP loaded with seed cells was implanted in the rabbit femur with 15 mm bone and periosteum defects. Fifty-four New Zealand white rabbits were randomly divided into three groups (n=18): sensory nerve bundle (group A) or motor nerve bundle (group B) were transplanted into the side groove of β-TCP scaffold, group C was used as a control without nerve bundle transplantation. X-ray detection was performed at the 4th, 8th, and 12th weeks after operation.
Citation： JIANG Shan,LIU Yong,WANG Qiushi,ZHAO Peiran,MU Tianwang,WANG Le,QIN Junjun,CHEN Siyuan,PEI Guoxian. EXPERIMENTAL STUDY ON CONSTRUCTION OF NEUROTIZATION TISSUE ENGINEERED BONE FOR REPAIRING LARGE BONE DEFECTS IN RABBIT. Chinese Journal of Reparative and Reconstructive Surgery, 2010, 24(5): 599-605. doi: Copy