WANG Weijun 1 , LI Sisheng 1 , NIU Dongsheng 2
  • 1Department of Orthopaedics, No.7 People’s Hospital of Zibo, Zibo Shandong, 255040, P.R.China;;
  • 2Department of Orthopaedics, the People’s Hospital of Ningxia Hui Autonomous Region.;
Nontraumatic avascular necrosis of the femoral head; Osteoporosis; Bone mineral density; Vascular endothel ial growth factor; Bone morphogenetic protein 2
Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables References Cited by

Objective To explore the significance and the relationshi p between osteoporosis and the mRNA expressions of vascular endothel ial growth factor (VEGF) and bone morphogenetic protein 2 (BMP-2) in nontraumatic avascular necrosis of the femoral head (NONFH), so as to provide a theoretical basis for the pathogenesis and the cl inical treatment of NONFH. Methods Sixty-nine specimens of femoral head were collected from voluntary donators undergoing total hi p arthroplasty, including 37 cases of NONFH (NONFH group) and 32 cases of fresh femoral neck fracture (control group). In NONFH group, there were 26 males and 11 females with an average age of 57.3 years (range, 43-75 years), including 19 cases of steroid-induced avascular necrosis of the femoral head (ANFH), 16 cases of alcohol ic ANFH, and 2 cases of idiopathic
ANFH; according to Ficat staging system, there were 23 cases at stage III and 14 cases at stage IV. In control group, there were 23 males and 9 females with an average age of 58.6 years (range, 46-79 years). The NO level of serum, the Q value of femur, and the bone mineral density (BMD) of weight-bearing area were measured firstly. The bone tissues were harvested from weightbearing necrosis area and healthy area. The pathological change was observed by HE staining, the percentage of empty bone lacuna and the percentage of trabecular bone area were calculated. The mRNA expressions of VEGF and BMP-2 in femoral head were detected through in situ hybridization technique. Results There were significant differences (P  lt; 0.05) in the NO level of serum, the Q value of femur, and the BMD between NONFH group and control group. In NONFH group, the femoral head showed irregular shape, the articular cartilage exfol iated and collapsed. In weight-bearing necrosis area, the bone trabeculae were sparse and non-intact with a great number of empty lacuna; necrotic bone trabeculae were decomposed and absorbed; no obvious bone regeneration and repair were observed. In weight-bearing healthy area, the fat cells in bone marrow showed prol iferation and hypertrophy. In control group, the femoral head had normal appearance, intact articular cartilage, and intact bone trabeculae with a regular arrange, and osteocytes were clearly seen. There were significant differences in the percentage of empty bone lacuna and the percentage of trabecular bone area between NONFH group and control group (P  lt; 0.05). The mRNA expressions of VEGF and BMP-2 were positive in 2 groups. The positive area ratio, the absorbance value, and integral absorbance
value of VEGF mRNA and BMP-2 mRNA in NONFH group were significantly lower than those in control group (P  lt; 0.05);
the grey scales of VEGF mRNA and BMP-2 mRNA in NONFH group were significantly higher than that in control group (P  lt;
0.05). Conclusion The pathological stage of osteoporosis may play an important role in the mechanism of the NONFH. The decrease of mRNA expressions of VEGF and BMP-2 in femoral head of NONFH is important reason that affect its bone mass, osteoporosis, rehabil itation, and reconstruction. It may be benefit to the reparative process of the necrosis femoral head to increase the mRNA expressions of VEGF and BMP-2 in the femoral head.

Citation： WANG Weijun,LI Sisheng,NIU Dongsheng. STUDY ON RELATIONSHIP BETWEEN OSTEOPOROSIS AND mRNA EXPRESSIONS OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND BONE MORPHOGENETIC PROTEIN 2 IN NONTRAUMATIC AVASCULAR NECROSIS OF FEMORAL HEAD. Chinese Journal of Reparative and Reconstructive Surgery, 2010, 24(9): 1072-1077. doi: Copy