LIU Xiaofeng 1,2# , ZHANG Zhengyao 1,2 , DENG Xuming 1 , GUO Yanchuan 3 , ZHOU Qiuli 2 , CHEN Lijuan 3 , ZHAO Wenjie 4 , SONG Yu 1
  • 1College of Animal Science and Veterinary Medicine, 2School of Pharmaceutical Sciences, Jilin University, Changchun Jilin, 130062, P.R.China;;
  • 3Technical Institute of Physics and Chemistry of Chinese Academy of Sciences;;
  • 4Jilin Natural Pharmatech Co.Ltd.;
Nano TCP/ gelatin / velvet antler polypeptide; Biocompatibility; Bone defect; Repair
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Objective To evaluate the biocompatibil ity of a new nano TCP/ gelatin / velvet antler polypeptide material. Methods The nano TCP/ gelatin / velvet antler polypeptide material was prepared, and the morphous was observed by scanning electron microscope. L929 and NIH/3T3 cell l ines were cultured conventionally. Acute toxicity test, hemolysis test, cell prol iferation and cytotoxicity test were used to evaluate the biocompatibil ity of the material. Results The composite
microsphere material was about 10 μm in diamerter and had good spherical geometry, high monodispersity with nanometer size holes on the surface. Toxic symptoms such as hyperspasmia, palsy and death did not appear during the observing stage in acute toxicity test. Maximum hemolysis rate of the material was less than 5% which met the requirement of hemolysis test standard as a medical material. Different concentrations of the materials leaching l iquor could enhance the prol iferation of NIH/3T3 cells, which showed the good biologic activity. Toxicity grade was 0, and the material was no cytotoxic. Conclusion Nano TCP/ gelatin / velvet antler polypeptide material has good biocompatibil ity.

Citation： LIU Xiaofeng,ZHANG Zhengyao,DENG Xuming,GUO Yanchuan,ZHOU Qiuli,CHEN Lijuan,ZHAO Wenjie,SONG Yu. BIOCOMPATIBILITY EVALUATION OF NANO TCP/ GELATIN/ VELVET ANTLER POLYPEPTIDE MATERIAL. Chinese Journal of Reparative and Reconstructive Surgery, 2009, 23(5): 598-601. doi: Copy