To explore the effect of hydroxybutyl chitosan on the prevention of postoperative peritoneal adhesion in rats. Methods Ninety SD rats (half males and half females) weighing 250-280 g underwent laparotomy with subsequent cecal wall abrasion and peritoneal adhesion. Rats were randomized into 3 groups (n=30 per group): group A, injection of 2 mL hydroxybutyl chitosan solution (2%); group B, injection of 2 mL sodium hyaluronate solution(2%); group C, the abdomen of rat was exposed for 30 seconds and served as control group. The general condition of the rats was observed after operation. The rats were killed 2 and 4 weeks after operation, 15 rats per group at a time, to undergo gross and histology
observation. The degree of adhesion was evaluated by double-bl ind method. The microstructure of injured electroscope cecal wall in groups A and C was observed with transmission electroscope 4 weeks after operation. Results All rats survived till the end of experiment. At 2 weeks after operation, the adhesion and the hyperplasia of fibrous connective tissue and collagen in groups A and B were sl ight while the adhesion in group C was serious with severe hyperplasia of fibrous connective tissue. According to the measurement classification by Nair histological grading, the difference between groups A and B and group C was significant (P lt; 0.05), while no significant difference was evident between group A and group B (P gt; 0.05). At 4 weeks after operation, the adhesion in group A was mild, and the hyperplasia of fibrous connective tissue and collagen were sl ight; the adhesion and the hyperplasia of fibrous connective tissue and collagen in group C were serious. The levels of group B were between group A and group C. The differences among three groups were significant (P lt; 0.05). Transmission electroscope showed inactive fibroblasts and loose thin collagen fibers in group A, and active fibroblasts and closely collagen fibers arranged in a disorderly manner in group C. Conclusion Hydroxybutyl chitosan can decrease the hyperplasia of fibrous connective tissue and inhibit the activity of fibroblasts significantly, and has a long-term role of preventing peritoneal adhesion.
Citation： LIU Shuangli,HOU Chunlin,WEI Changzheng,YIN Guodong,GUO Xingfeng,YIN Gang.. PREVENTION EFFECT OF HYDROXYBUTYL CHITOSAN ON PERITONEAL ADHESION IN RATS. Chinese Journal of Reparative and Reconstructive Surgery, 2009, 23(6): 718-722. doi: Copy