LIU Bingli 1,2 , LI Zirong 3 , SUN Wei 3 , WANG Bailiang 3 , SHI Shaohui 3 , MIN Hongwei 3
  • 1Graduate College of Peking Union Medical College, 100730, P.R.China;;
  • 2Department of Joint,Tangshan No.2 Hospital;;
  • 3Department of Orthopedics, China-Japan Friendship Hospital.;
Osteonecrosis of the femoral head; Alcohol; Methylene tetrahydrofolate reductase; Single nucleotide polymorphism
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Objective To explore the relationship between alcohol induced osteonecrosis of the femoral head (ONFH) and the single nucleotide polymorphisms (SNP) of methylene tetrahydrofolate reductase (MTHFR) 677 C/T. Methods From July 2005 to May 2008, eighty-nine male patients with alcohol induced ONFH were selected as the patient group, aged from 24 to
58 years old (mean 44.3 years old). The time of drinking was about 17 years, 375 mL/day. The imaging evidence showed ONFH with no other history associated to ONFH. Seventy-seven male healthy adults were selected as the control group, aged from 23 to 52 years old (mean 42.7 years old). The time of drinking was about 14 years, 335 mL/day. The imaging evidence showed no ONFH. The 2 mL blood sample was acquired from every subject. DNA was purified from leucocyte at first, then was ampl icated by PCR, the product of PCR was sequenced at last. The SNP of MTHFR 677 C/T was analyzed with SPSS 12.0 software package. Results The TT genotype and T allele frequencies of MTHFR 677 C/T were 27.2% and 52.0% (P  gt; 0.05) in the control group, and the distribution of genotype was consistent with Hardy-Weinberg equil ibrium. The genotype frequencies of CC, CT and TT were 23.4% (18 cases), 49.4% (38 cases) and 27.2% (21 cases) in the control group, were 14.6% (13 cases), 36.0% (32 cases) and 49.4% (44 cases) in the patient group; showing statistically significant differences (P  lt; 0.05). The allele frequencies of C and T were 48.0% (74) and 52.0% (80) in the control group, and were 32.6% (58) and 67.4% (120) in the patient group; showing statistically significant differences (P  lt; 0.05). The frequencies of C, T alleles and TT genotype were higher in the patient group than in the control group, showing statistically significant differences (P  lt; 0.05). The odds ratios were 0.523, 1.914 and 2.607, respectively; the 95% confidence interval were 0.335-0.816, 1.226-2.987, 1.359-5.001, respectively. Conclusion The relationship may exist between the SNP of MTHFR 677 C/T and alcohol induced ONFH.

Citation： LIU Bingli,LI Zirong,SUN Wei,WANG Bailiang,SHI Shaohui,MIN Hongwei. RELATIONSHIP BETWEEN ALCOHOL INDUCED OSTEONECROSIS OF FEMORAL HEAD AND SINGLENUCLEOTIDE POLYMORPHISMS OF METHYLENE TETRAHYDROFOLATE REDUCTASE 677 C/T. Chinese Journal of Reparative and Reconstructive Surgery, 2009, 23(9): 1079-1082. doi: Copy