【Abstract】 Objective To improve the method of obtaining purified and viable human hair foll icle stem cells (HFSCs)
from bulge cells (BCs). Methods Firstly, the BCs were isolated from human hair foll icles by microdissection. Secondly, the CD200+ HFSCs were selected from BCs using magnetic cell sorting method. The viabil ity of these purified HFSCs was detected under l ight microscope. The purification rate was analyzed by flow cytometry. The pre- and post-purification cells were compared by immunofluorescence staining. Results The adherent BCs displayed a typical cobblestone morphology on day 6. The BCs expressed K19 bly. The viabil ity rate of pre-purification cells was 95.0% ± 0.6% while that of post- purification cells was 94.2% ± 1.0%. There was no significant difference (P lt; 0.05). By flow cytometry and immunofluorescence staining examination, the CD200+ cell rate was 8.31% before cell sorting purification while that was 82.31% after cell sorting purification. Con clu sion Highly purified and viable HFSCs could be obtained by micromanipulation and magnetic cell sorting assay.
Citation： TAN Ting,HU Zhiqi,ZHOU Hongjun.. SEPARATION AND PURIFICATION OF HUMAN HAIR FOLLICLE STEM CELLS BY MICROMANIPULATION ANDMAGNETIC CELL SORTING. Chinese Journal of Reparative and Reconstructive Surgery, 2008, 22(2): 202-205. doi: Copy