YU Deshui 1 , LV Gang 1 , WANG Yanfeng 1 , LIU Libo. 2
  • 1Department of Orthopaedics, the First Affiliated Hospital, Liaoning Medical College, Jinzhou Liaoning, 121001, P.R.China;;
  • 2Department of Neurobiology, College of Basic Medical Sciences, China Medical University.;
Spinal cord injury; BMSCs transplantation; Fetal l iver kinase 1; VEGF receptor
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Objective To observe the effect of BMSCs transplantation on gene and protein expression of VEGF receptor fetal l iver kinase 1 (Flk-1) after spinal cord injury (SCI), and to investigate the mechanism of repairing the SCI by BMSCs transplantation. Methods BMSCs were isolated and cultured from five 4-week-old male Wistar rats weighing
100-120 g. The SCI model was made by using the modified Allen’s impactor device. Eighty-one adult female Wistar rats weighing 220-250 g were randomly divided into 3 groups: sham-operated group (group A, n=21), in which spinous process and vertebral plate of thorax 8-10 spinal cord segment were removed; DMEM group (group B, n=30), in which rats received four injections of DMEM in the peri-lesion area; and BMSCs group (group C, n=30), in which rats received four injections of BMSCs in the peri-lesion area. The changes of Flk-1 mRNA expression in rats’ spinal cord tissues were detected with RT-PCR method 1, 3 and 5 days after transplantation. The expression of Flk-1 protein was observed by using immunohistochemical technology in spinal cord 3, 7 and 14 days after transplantation. Results Morphology of the primary cultured BMSCs was various. Cell morphology tended to be uniform with the accumulation of passages, which appeared flat and spindle-shaped. RT-PCR results showed that there was no significant differences (P  gt; 0.05) in Flk-1 mRNA expression between group C and group B at different time points after transplantation. But Flk-1 mRNA levels of group B and group C significantly increased and peaked 1 day after transplantation (P  lt; 0.01), and then decreased 3 days after transplantation (P  lt; 0.01) compared with that of group A, and were still higher than that of group A 5 days after transplantation (P  lt; 0.05). Immunohistochemical staining results revealed that the expression of Flk-1 in group B was enhanced 3 and 7 days after transplantation compared with group A, which was significantly different (P  lt; 0.01). There was no significant difference in the expression of Flk-1 between group B and groupp A 14 days after transplantation (P  gt; 0.05). There was no significant difference in Flk-1 protein expression between group C and group B 3 days after transplantation (P  gt; 0.05). The expression of Flk-1 protein in group C was significantly higher than that in group B 7 and
14 days after transplantation (P  lt; 0.01). Conclusion BMSCs transplantation after SCI does not have regulatary effect on
the expression of Flk-1 mRNA, but it does upregulate the Flk-1 protein expression, which may be one of the mechanisms of repairing SCI.

Citation： YU Deshui,LV Gang,WANG Yanfeng,LIU Libo.. EFFECT OF BMSCs TRANSPLANTATION ON VEGF RECEPTOR FETAL LIVER KINASE 1 AFTER SPINAL CORD INJURY IN RATS. Chinese Journal of Reparative and Reconstructive Surgery, 2008, 22(8): 974-978. doi: Copy