• Department of Orthopaedic Surgery, Nanfang Hospital, the First Military Medical University, Guangzhou Guangdong, 510515, P. R. China;
Nacre/Polylactic acid artificial bone; Osteoblasts; Ectopic osteogenesis; Scaffold material
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Objective To study the mechanism of ectopic osteogenesis of nacre/Polylactic acid (N/P) artificial bone combined with allogenic osteoblasts, and to explore the possibility as a scaffold material of bone tissue engineering. Methods The allogenic- osteoblasts seeded onto N/P artificial bone were co-cultured in vivo 1 week.The N/P artificial bone with allogenic osteoblasts were implanted subcutaneously into the left back sites of the New Zealand white rabbits in the experimental group and the simple N/P artificial bone into the right ones in the control group. The complexes were harvested and examined by gross observation, histologic analysis and immunohistochemical investigation 2, 4 and 8 weeks after implantation respectively.Results In experimental group, the osteoid formed after 4 weeks, and the mature bone tissue withbone medullary cavities formed after 8 weeks; but in control group there was nonew bone formation instead of abundant fibrous tissue after 4 weeks, and more fibrous tissue after 8 weeks.Conclusion N/P artificial bone can be used as an optical scaffold material of bone tissue engineering.

Citation： TANG Huanzhang,CHEN Jianting,JINDadi,et al.. IN VIVO ECTOPIC OSTEOGENESIS OF NACRE/POLYLACTIC ACID ARTIFICIAL BONECOMBINED WITH ALLOGENIC OSTEOBLASTS. Chinese Journal of Reparative and Reconstructive Surgery, 2005, 19(2): 114-117. doi: Copy