Objective To observe the changes of immune status in recipient after implanting with xenogeneic acellular bone matrix (ACBM). Methods Twenty rabbits were randomly divided into 4 groups. Autograft，ACMB and bone soaked in alcohol were implant into the 3 experimental groups separately, and No-treatment was done as control group. The CD4+,CD8+,CD25+T lymphocytes in blood were detected by flow cytometer at 1, 2, 4 and 6 weeks after operation. After 2 and 6 weeks of implantation, the changes of bone and tissue were observed by histology. Results After 2-6 weeks, CD4+ and CD8+ T cells were significantly higher in the implantedgroup of bone soaked in alcohol than that in the other 3 groups(P lt;0.05) and there wasno statistically significant difference in the other 3 groups(P gt;0.05). After 2 weeks, CD25+ T cells were significantly higher in the implanted group of bone soakedin alcohol than that in the other groups. In the 2nd week, there were inflammatory infiltration with a predominance of granulocytes. In the 6th week, there were many fibroblasts instead of granulocytes with a few lymphocytes and cartilage island formed in the implanted groups of autograft and ACMB. Conclusion ACBM implanting has low influence on cellular immunity in recipient.

Citation： SUN Xinjun,WANG Zhengguo,ZHU Peifang,et al.. CHANGES OF SUBTYPE T LYMPHOCYTES IN BLOOD AFTER IMPLANTING WITH XENOGENEIC ACELLAR BONE MATRIX. Chinese Journal of Reparative and Reconstructive Surgery, 2005, 19(4): 322-323. doi: Copy