• Department of Orthopedic Surgery, the Third Hospital of Peking University. Beijing, P. R. China 100083.;
Tissue engineering bone; Marrow mesenchymal stem cells; Osteogenic induction; Osteogenic differentiation
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Objective To study the method of inducing human marrow mesenchymal stem cells (MSCs) into osteoblasts directionally and to identify osteogenesis characteristics. Methods MSCs were isolated from adult marrow using density gradient separation method and were cultured in conditioned medium containing Dex 10 -8 mol/L，β-GP 10 mmol/L，and AA 50 μg/ml. The MSCs attachment formed soon and passage 3 cells were chosen to check osteogenesis characteristics, including alkaline phosphatase assay with modified calcium-cobalt staining method, type Ⅰ collagen assay with immunohistochemistry, osteopontin and osteonectin assay with in situ hybridization and calcium nodes assay with Von Kossa staining. Results Passage 3 MSCs had typical appearance of osteoblasts and could be passaged continuously till passage 10. The rate of ALP expression was 85%. The expressions of collagen type Ⅰ, osteopontin and osteonectin were positive and calcium nodeswere seen by Von Kossa staining. Conclusion We have successfully induced human MSCs into osteoblasts; the induced cells have typical osteogenesis characteristics.

Citation： YIN Xiaoxue,CHEN Zhongqiang,GUO Zhaoqing,et al.. INDUCING HUMAN MARROW MESENCHYMAL STEM CELLS INTO OSTEOBLASTS DIRECTIONALLY AND IDENTIFICATION OF THEIR OSTEOGENESIS CHARACTERISTICS. Chinese Journal of Reparative and Reconstructive Surgery, 2004, 18(2): 88-91. doi: Copy