• YANG Xue yi;;
  • CAO Bin yun;;
  • FENG Xiu liang;;
  • et al.;
Key Laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology of Agricultural Ministry of China; Northwest Science and Technology University of Agriculture and Forestry. Yangling Shaanxi; P. R. China 712100
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Objective To establish a good method and culture system to isolate skin stem cell and expand it in vitro so as to lay a foundation for exploring the proliferation and differentiation mechanism of skin stem cell. Methods Skin stem cells were obtained by explant culture and identified by using alkaline phosphatase(ALP) staining and differentiating experiment in vitro. Stem cell was induced by the cocktail of conditional medium with cell growth factor (insulin like growth factor and epidermal growth factor). Results Skin stem cell colonies were derived from ear skin tissues of adult dairy goats. The colonies had some characteristics of embryonic stem cells, such as the ability to be continously passaged (Passage 5) and the morphology nest-like. They continued to be ALP positive and had the capacity of forming embryoid bodies. These cells were pluripotent and stem-like cells. In vitro these stem cell can be induced to be Follicle-like structure, Astrocyte-like cells, osteoblast-like cell. Conclusion Explant culture is a good method to isolate skin stem cell, which can be induced to be committed differentiation and trans-differentiation.

Citation： CLONING AND INDUCED DIFFERENTIATION OF ADULT GOAT SKIN STEM CELLS IN VITRO. 成年山羊皮肤干细胞体外克隆与诱导分化. Chinese Journal of Reparative and Reconstructive Surgery, 2003, 17(2): 93-96. doi: Copy