• Department of Plastic Surgery;;
  • the Second Hospital of Harbin Medical University. Harbin Heinongjiang;;
  • P. R. China 150086;;
Tissue engineering; Vascular graft; Extracellular matrix; Vascular endothelial cell; Seeding
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Objective To develop a new method for a tissue engineered vascular graft by combining endothelial cells and an acelluarized allogenic matrix. Methods Acellularized matrix tubes were obtained by a 0.1% trypsin and 0 02% EDTA solution for 24 hours and 1% Triton X 100 for 176 hours, respectively. Endothelial cells were isolated from alloaorta and expanded in vitro. Finally, the inner surface of acellularized matrix was reseeded with endothelial cells. Acellularity and reseeding were analysed by light microscopy and scanning electron microscopy. Results The acellularization procedure resulted in an almost complete removal of the original cells and the loose three-dimensional (3D) matrix. The acellular matrix could be reseeded with expanded endothelial cells in vitro, and endothelial cells had the potential of spread and proliferation. Conclusion Acellular matrix produces by Tritoon X-100 and trypsin possesses satisfactory biocompatibility for allogenic endothelial cell. Vascular grafts can be generated in vitro by a combination of endothelial cells and allogenic acelluarized matrix.

Citation： GUO Tie fang,YANG Da ping,HAN Xue feng,et al.. EXPERIMENTAL STUDY OF COMPATIBILITY BETWEEN ACELLUARIZED ALLOGENIC MATRIX AND ENDOTHELIAL CELL IN VITRO. Chinese Journal of Reparative and Reconstructive Surgery, 2003, 17(2): 165-168. doi: Copy