• Institute of Hematology;;
  • Union Hospital;;
  • Tongji Medical College;;
  • Huazhong University of Science &;
  • Technology. Wuhan Hubei;;
  • P.R.China 430022;;
Tissue Engineering; Chondrocytes; Apoptosis; caspase 3
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OBJECTIVE: To investigate apoptosis of chondrocytes cultured in vitro and related expression of caspase-3. METHODS: Apoptosis of chondrocytes were detected by flow cytometry analysis and TUNEL staining. The expression of caspase-3 was determined by RT-PCR and Western blot, and caspase-3 protein activity was determined by ELISA. RESULTS: Apoptosis was observed in chondrocytes cultured in vitro from passage 1 to passage 4 at various degrees. The percentage of apoptosis of chondrocytes on day 7 was much higher than that on day 3 (15.7% +/- 0.3% vs 8.9% +/- 0.6%, P  lt; 0.01). caspase-3 mRNA and protein expressed in chondrocytes during whole culture process. Along with the culture time extension in vitro, caspase-3 expression and protein activity up-regulated, coincident with apoptosis of chondrocyte. caspase-3 was activated and a fragment of 20 kDa was detected after 7 days of culture. CONCLUSION: caspase-3 is involved in apoptosis of chondrocytes cultured in vitro.

Citation： XIAO Juan,CAO Yi lin,FAN Hua hua,et al.. APOPTOSIS OF FETUS CHONDROCYTES CULTURED IN VITRO AND EXPRESSION OF CASPASE-3. Chinese Journal of Reparative and Reconstructive Surgery, 2003, 17(5): 418-421. doi: Copy