• Department of Oral Histology &;
  • Pathology;;
  • Qin Du Stomatological College;;
  • the Fourth Military Medical University. Xi an Shaanxi;;
  • P.R.China 710032;;
Tissue engineering skin; Melanocytes; Cell culture
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OBJECTIVE: To construct a tissue engineering skin containing melanocytes by employing tissue engineering method. METHODS: The keratinocytes, dermal fibroblasts and melanocytes were isolated and purified. Then the cells were used to construct a tissue engineering skin containing melanocytes. The location of melanocytes in the tissue engineering skin were detected by Dopa staining, transmission electron microscope (TEM) and S-100 immunohistochemical staining. RESULTS: Melanocytes can be detected in the basal layer of the constructed tissue engineering skin. The results of TEM showed that the melanocytes were in good conditions. CONCLUSION: The artificial skin containing melanocytes was successfully constructed in vitro and can be used to repair the full-thickness skin defects.

Citation： LIU Yuan,JIN Yan,WANG Xinwen,et al.. CONSTRUCTION OF TISSUE ENGINEERING SKIN CONTAINING MELANOCYTES. Chinese Journal of Reparative and Reconstructive Surgery, 2003, 17(6): 501-503. doi: Copy