• Institute of Cancer;;
  • West China Hospital;;
  • Sichuan University. Chengdu Sichuan;;
  • P. R. China 610041;
Human fibroblasts; Transforming growth factor-β1; Autocrine
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OBJECTIVE: To explore the autocrine regulation for the transforming growth factor-beta 1(TGF-β1) by the fibroblasts in vitro. METHODS: Fibroblasts were cultured in vitro which isolated from the normal prepuce by circumcision. TGF-β1 concentration were determined with ELISA for the different passages and the different time at the same passage. RESULTS: The TGF-β1 concentration was different for the distinct passage of fibroblasts and achieved the peak (450 ng/L) at the sixth passage. Among the same fibroblasts, the TGF-β1 concentration was different in the various days and passage of the summit (680 ng/L) on the fifth day, which was 2.5 times as high as that of the first day. CONCLUSION: The fibroblasts possess the ability of autocrine of the TGF-β1 and have some regularity. It will provide the theory basis for the research about TGF-β1 regulation mechanism and the medical application about salvia miltiorrhiza.

Citation： ZHANG Jie,LIN Ping,HUANG Lu gang,et al.. AUTOCRINE REGULATION FOR TRANSFORMING GROWTH FACTOR-β_1 BY FIBROBLAST IN VITRO. Chinese Journal of Reparative and Reconstructive Surgery, 2002, 16(5): 319-321. doi: Copy