• Department of Plastic and Reconstructive Surgery;;
  • Shanghai 9th People s Hospital;;
  • Shanghai Second Medical University. Shanghai;;
  • P. R. China 200011.;;
Transforming growth factor β1; Autoproduction; Signal transduction; Gene therapy
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OBJECTIVE: To study the effect of overexpression of truncated type II TGF-beta receptor on transforming growth factor-beta 1(TGF-beta 1) autoproduction in normal dermal fibroblasts. METHODS: In vitro cultured dermal fibroblasts were treated with recombinant human TGF-beta 1(rhTGF-beta 1) (5 ng/ml) or recombinant adenovirus containing truncated type II TGF-beta receptor gene (50 pfu/cell). Their effects on regulating gene expression of TGF-beta 1 were observed with Northern blotting. RESULTS: rhTGF-beta 1 up-regulated the gene expression of TGF-beta 1 and type I procollagen. Overexpression of truncated receptor II down-regulated the gene expression of TGF-beta 1. CONCLUSION: Overexpression of the truncated TGF-beta receptor II decreases TGF-beta 1 autoproduction via blocking TGF-beta receptor signal. The results may provided a new strategy for scar gene therapy.

Citation： LIU Wei,CAI Ze hao,SHANG Qing xin,et al.. OVEREXPRESSION OF TRUNCATED TYPE Ⅱ TRANSFORMING GROWTH FACTOR-β RECEPTOR IN DERMAL FIBROBLASTS. Chinese Journal of Reparative and Reconstructive Surgery, 2002, 16(6): 404-407. doi: Copy