OBJECTIVE The expression and distribution of human blood type A, B, O antigens in Chinese Neijiang porcine were investigated. METHODS The anti-human blood type A, B, H monoclonal antibodies were used as the primary antibody, and the immunohistochemistry EnVision technique was used to detect the expression of A, B, H antigens in tissues from 10 pigs. RESULTS The B and H antigens were not found in all 10 pigs’ tissues investigated. While the A antigen was found in all above tissues except vascular endothelial cells. CONCLUSION The A antigen did not exist on vascular endothelial cells, so it did not play a role as a hyperactive antigens in pig to human xenotransplantation. However, in other tissues it might play roles in acute cellular rejection or chronic rejection. The future study on its role in pig to human xenotransplantation was needed. The expression of human blood type antigens in Neijiang pigs was different from other pig strains reported, it was suggested that it was possible to find a pig strain that contained less xenoantigen.

Citation： BU Hong,CHEN Yun ying,LI You ping,et al.. EXPRESSION AND DISTRIBUTION OF HUMAN BLOOD TYPE ANTIGENS IN CHINESE NEIJIANG PIG TISSUES. Chinese Journal of Reparative and Reconstructive Surgery, 1999, 13(2): 109-113. doi: Copy