梁小玲 1 , 杨诚 2 , 周焕娇 3 , 魏丽清 3 , 林佩蓉 2 , 杨璐 2
梁小玲, Email: liangxlsums@yahoo.com.cn
Endothelial cells; Cells, cultured/drug effects; Tetramethylpyrazine/therapeutic use; Vascular endothelial growth factors
Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables References Cited by

Objective  To observe the effect of tetramethypyrazine (TMP) on the expression of hypoxia-related factors in human umbilical vein endothelial cells (HUVECs). Methods  The second to fifth passage cultured HUVECs were divided into five groups: control group, CoCl2induced hypoxic group and 50, 100, 200  mu;mol/L TMP treatment groups. HUVECs in control group were not treated. HUVECs inCoCl2induced hypoxic group were treated with 150  mu;mol/LCoCl2for four hours. HUVECs in 50, 100, 200  mu;mol/L TMP treated groups were pretreated with 150  mu;mol/LCoCl2 for four hours, followed by treatment with 50, 100, 200  mu;mol/L TMP for eight hours. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA levels of prolyl hydroxylase 2 (PHD2), hypoxia-induced factor-1 alpha;(HIF-1 alpha;) and vascular endothelial growth factor (VEGF). Protein levels of PHD2, HIF-1 alpha;, and VEGF were detected using Western blot. Results  Compared with the control group, theCoCl2 induced hypoxic group showed decreased mRNA and protein levels of PHD2 (t=3.734, 3.122;P＜0.05), while those of HIF-1 alpha; and VEGF increased (HIF-1 alpha; mRNA:t=4.589,P＜0.05; HIF-1 alpha; protein:t=3.778,P＜0.05. VEGF mRNA:t=3.926,P＜0.05; VEGF protein:t=3.257,P＜0.05). Compared with theCoCl2 induced hypoxic group, 50, 100, 200  mu;mol/L TMP treated groups showed increased mRNA and protein levels of PHD2 (PHD2 mRNA: t=3.286, 3.617, 3.886;P＜0.05. PHD2 protein: t=2.813, 3.026, 3.078; P＜0.05); while those of VEGF decreased (VEGF mRNA: 50  mu;mol/L TMP: t=1.696,P＞0.05; 100  mu;mol/L TMP:t=2.974,P＜0.05; 200  mu;mol/L TMP: t=3.492,P＜0.05; VEGF protein: 50  mu;mol/L TMP: t=1.986,P＞0.05; 100  mu;mol/L TMP: t=2.976,P＜0.05; 200  mu;mol/L TMP:t=3.136,P＜0.05); although changes in HIF-1 alpha;mRNA levels were not statistically significant (t=1.025, 0.726, -1.386;P＞0.05), showed a decrease in HIF-1 alpha;protein levels (50  mu;mol/L TMP: t=2.056,P＞0.05; 100  mu;mol/L TMP:t=3.058,P＜0.05; 200  mu;mol/L TMP:t=3.828,P＜0.05). ConclusionIn  HUVECs, TMP can upregulate the mRNA and protein expression of PHD2, while down regulating HIF-1 alpha; protein expression and VEGF mRNA and protein expression under acute hypoxic conditions.

Citation： 梁小玲,杨诚,周焕娇,魏丽清,林佩蓉,杨璐. Effect of tetramethypyrazine on hypoxia-related factors expression in human umbilical vein endothelial cells. Chinese Journal of Ocular Fundus Diseases, 2013, 29(3): 284-289. doi: Copy