Objective 　To observe the effect of CD44 antibody on the hyaluronic acid (HA) degradation mediated by retinal M uuml;ller cell. Methods 　Pig retinal M uuml;ller cells from the posterior pole (2nd generation) were cultured in three different medium：without HA (group 1)，0.01 mg/ml HA (group 2)，10  mu;g/ml HA and CD44 antibody （group 3）. The cells in the group 2 and 3 were precultured with HA and CD44 antibody，and the supernatant was collected. HA-substrate gel electrophoresis was performed for HA degradation，while ELISAlike method was performed for HA-binding protein. Results 　HA-substrate gel electrophoresis showed white light double-band on blue background in groups 1 and 3，thicker double-band or bright de-colored blocks in group 2. ELISA-like method showed that the absorbance (A) value of groups 1, 2 and 3 were 0.310 plusmn;0.025， 0.093 plusmn;0.051，0.025 plusmn;0.069 respectively. The A value of group 2 was obviously lower than that of group 1 （t=28.1,P＜0.01）；the A value of group 3 was significantly higher than that of group 2 （t=26.9,P＜0.01），but was the same as group 1 （t=4.92,P＞0.05）. Conclusion 　CD44 antibody can inhibit the interaction between M uuml;ller cells and HA, and thus reduce the HA degradation.

Citation： 陆融,乔伟振. Effect of CD44 antibody on hyaluronic acid degradation caused by retinal Müller cel. Chinese Journal of Ocular Fundus Diseases, 2011, 27(2): 160-162. doi: Copy