Objective 　To observe the effect of cyanin on the expression of L-glutamate/L-aspartate transporter (GLAST) in high glucose cultured retina M uuml;ller cells. Methods 　The retinal tissue of Sprague-Dawley (SD) rats was collected at postnatal 10 day, and M uuml;ller cells were isolated and cultured according to literature. The M uuml;ller cells (2nd 4th generations) were treated with five different medium as normal group (group A), high glucose control group (group B), high glucose＋30  mu;mol/L cyanin group (group C), high glucose＋60  mu;mol/L cyanin group (group D) and high glucose＋100  mu;mol/L cyanin group (group E). Cell relative survival rates (A value) were measured by MTT assay at 570 nm.The GLAST protein expression in M uuml;ller cells was observed by Western blot. Results 　MTT assay showed that the A value of the five group were 0.450 8 plusmn;0.020 4, 0.270 1 plusmn;0.031 4, 0.332 0 plusmn;0.023 2, 0.428 3 plusmn;0.017 2, 0.361 9 plusmn;0.027 0,the cell relative survival rate were 100.0%, 59.9%, 73.6%, 95%, 80.3% respectively. The A value of group C，D，E were significantly higher than that of group B (F=32.25,P＜0.05)，the A value of group D were significantly higher than that of group C and E (F=21.07,P＜0.05).Western blot showed that the GLAST protein expression of group B was lower than that of group A (t=5.25,P＜0.05)；there was no obvious changes of GLAST protein expression in group A，C，D and E (F=2.979, P＞0.05). Conclusion 　Cyanin can rescue high glucose induced GLAST reduction.

Citation： 钱志刚,柯敏. Effect of cyanin on the expression of L-glutamate and L-aspartate transporter in high glucose cultured retina Müller cells. Chinese Journal of Ocular Fundus Diseases, 2011, 27(2): 170-173. doi: Copy