Export PDF Favorites Scan Get Citation

Objective To observe the toxic effects of intravitreal injection of etoposide on retinal ganglion cells (RGC) in rabbit eyes. Methods Twenty-four healthy rabbits were divided into 8 groups randomly, including one normal group without any treatment, and 7 experimental groups with intravitreal injection of different dosages of etoposide (0.0, 1.5, 15.0, 150.0, 375.0, 750.0, 1500.0  mu;g).Twelve days after the injection, the animals were killed by air embolism, and the eyeballs were prepared for routine pathological examination with hematoxylineosin staining and transmission electron microscopy. Results Four experimental groups (0.0, 1.5, 15.0, 150.0  mu;g etoposide) had normal retinal structure and normal shape of ganglion cells while other 3 experimental groups with higher dosage of etoposide (375.0, 750.0 and 1500.0  mu;g) showed thinner and disorganized retina. The number of RGC of the normal group and 0.0, 1.5, 15.0, 150.0, 375.0, 750.0, 1500.0  mu;ggroups were 61.66 plusmn;3.72, 61.83 plusmn;4.59, 60.00 plusmn;7.07, 62.00 plusmn;4.43, 62.67 plusmn;3.98, 7.33 plusmn;1.37, 0.00 and 0.00 per 400 times;visual field, with statistical difference among groups (F=145.04,P=0.00). There was no statistical difference between the normal group and 0.0, 1.5, 15.0, 150.0  mu;g groups (F=0.244,P>0.05). The number of RGC of 375.0  mu;g group was decreased obviously compared with normal group and 0.0, 1.5, 15.0, 150.0  mu;g groups with statistical difference(F=145.04,P<0.05). Conclusion Etoposide has a dosage-dependent toxicity on rabbit RGCs.

Citation: 郑嵩山,卜战云,王应飞,吴中耀. Toxic effects of intravitreal injection of etoposide on retinal ganglion cells in rabbit eyes. Chinese Journal of Ocular Fundus Diseases, 2011, 27(4): 366-369. doi: Copy