Objective To observe the inhibitory effect of recombined adenovirus of p21 (rAd-p21) on the proliferation of Rhesus retinal vascular endothelial cell line (RF/6A). Methods RF/6A cells were cultured in vitro which divided into phosphate buffered solution (PBS) group, rAd-p21 transfection group and negative control (NC) group. Plasmid vectors were transfected into RF/6A cells. The expression of p21 mRNA and protein in RF/6A cells were measured by RT-PCR and Western blot respectively. The cell cycle distribution was analyzed by flow cytometry. Endothelial-cell tube formation assay was performed in Matrigel.Results The expression of p21 mRNA and protein in rAd-p21 transfection group were higher than that in PBS and negative control group. The cell cycle distribution showed that the proportion of G0/G1 cells in rAd-p21 transfection group ［(67.45 plusmn;11.61) %］ was apparently higher than that in the negative control group ［(41.55 plusmn;8.99)%］ and PBS group ［(40.76 plusmn;6.66) %］ (F=21.284, P=0.000). The number of endothelialcell tubes in the rAd-p21 transfection group (3.86 plusmn;1.21) was apparently less than that in the negative control group (7.62 plusmn;2.69) and PBS group (8.25 plusmn;3.19) (F=7.138，P=0.004). Conclusions The p21 mRNA and protein can stably express in RF/6A cells after rAd-p21 transfection. RAd-p21 can inhibit the proliferation of RF/6A cells.
Citation： 韩金栋,袁志刚,郑华宾,颜华. Recombined adenovirus of p21 inhibit the proliferation of Rhesus retinal vascular endothelial cells in vitro. Chinese Journal of Ocular Fundus Diseases, 2011, 27(5): 462-465. doi: Copy