陈瑛 1 , 马进 2 , 朱铁培 2
  • 1. 2. e Center of Affiliated Second Hospital School of Medicine Zhejiang University;
Diabetic retinopathy/Phisiopathology; Gene expression/genetics; Diabetes mellitus,experimental
Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables References Cited by

Objective　To observe the opticin expression in the eyes of nonobese diabetes (NOD) mice and nondiabetic NOD mice.Methods　Twenty NOD mice were divided into diabetic group (experimental group) and nondiabetic group (control group). All the mice were killed by cervical dislocation method.The eyes were harvested, and the vitreous, retina and sclera were separately collected. Western blot and realtime reverse transcriptionpolymerase chain reaction（RT-PCR）were respectively used to determine opticin protein and OPTC mRNA levels.Results　The opticin protein level in the vitreous and retina was lower in the experimental group（t=4.42,4.58;P=0.002,0.002）,but is same in thesclera between the 2 groups（t=0.27,P=0.794）.OPTCmRNA level was vitreous gt;retina gt;sclera. OPTCmRNA levels of vitreous and retina in diabetic group were significantly lower（t=3.30,2.48；P=0.01,0.04）; there was no statistical significant on OPTC mRNA of sclera between two groups（t=0.27,P=0.80）.Conclusion　Expression of opticin was suppressed in retina and vitreous of diabetic mice.

Citation： 陈瑛,马进,朱铁培. Expression of opticin in the eyes of diabetic mice. Chinese Journal of Ocular Fundus Diseases, 2010, 26(2): 143-146. doi: Copy