Objective To observe the different effect such as high concentration of glucose and high concentration of insulin on GLUT1 of Rabbit Retinal M uuml;ller Cell in vitro. Methods Rabbit retinal M uuml;ller cells were cultured in vitro with suspended constitution，which were divided as the following groups: common control group，high glucose group，insulin group，high glucose combined insulin group. Laser confocal microscope combined with immunocytochemical and fluorescence staining method to quantitatively analyze the expression condition of GLUT1. Results The expression of GLUT1 has been enhanced obviously by high glucose and high insulin，which locates mainly in the cytoplasm that near to the nucleus. Conclusion Rabbit retinal M uuml;ller cells can express GLUT1，and the expression of GLUT1 can be reinforced by high glucose and high insulin. （Chin J Ocul Fundus Dis，2008，24：265-267）
Citation： E Song Xiaokang Guan Qiuli Wang Yakun Wang Sheng Ju Mingchao Bi. Study of expression and effect factors on glucose transporter-1 of rabbit retinal Müller cells cultured in vitro. Chinese Journal of Ocular Fundus Diseases, 2008, 24(4): 265-267. doi: Copy