Changzheng CHEN Meng LI Yiqiao XING Wei Hou Jing CHEN Ying LI
  • Department of Ophthalmology, Renming Hospital of Wuhan Uni bersity, Wuhan 430060, China Department of Ophthalmology, Renming Hospital of Wuhan Uni bersity, Wuhan 430060, China Department of Ophthalmology, Renmin hospital of Wuhan University Department of Ophthalmology, Renmin hospital of Wuhan University;
Glycoproteins/ physiology; Tissue distribution; Vitreous body/ physiology
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Objective To prepare a new glycoproteinopticin specific antibody and to explore the distribution of opticin in human eye. Methods Firstly, take the opticin specific antibody to compound a synthetic peptide chain（CLPRLPIGRFT）, and then get the opticin antibody. To verify the availability of antibody through the western blot for human vitreous extract, to test the distribution of opticin in human eye by immunohistochemistry. Results Through the western blot for human vitreous extract, we can see a band with wild range at molecular weight 45 times;103~50  times;103. We find that opticin exact in retina, vitreous and non-pigmented epithelium of ciliary body which distributes along the collagen fibrils in vitreous. Conclusion The availability of the antibody was confirmed by western blot. Opticin are mainly in retina, vitreous and nonpigmented epithelium of ciliary body. Opticin distributes along the collagen fibrils which may be related to the stability of vitreous. （Chin J Ocul Fundus Dis，2008，24：286-288）

Citation： Changzheng CHEN Meng LI Yiqiao XING Wei Hou Jing CHEN Ying LI. Distribution of new glycoprotein-opticin in human eye. Chinese Journal of Ocular Fundus Diseases, 2008, 24(4): 286-288. doi: Copy